Freshly isolated spleen-derived DO11.10 CD4+ T cells from IRF5+/+, IRF5+/−or IRF5−/−mice at 0.2×106 cells/well on 24 well plates were stimulated with 5 μg/ml anti-CD3 (145–2C11, Bio X Cell, West Lebanon, NH) and 1 μg/ml anti-CD28 (PV-1, Bio X Cell) antibody-coated wells for 72 hours. The following were assessed by ELISA: from BD biosciences: anti-IL2, biotin anti-IL2, anti-IFNγ, biotin anti-IFNγ, anti-TGFβ, biotin anti-TGFβ; from ThermoFisher Scientific: anti-IL17, biotin anti-IL17, biotin anti-IL5, anti-IL13, biotin anti-IL13; from BioLegend: anti-IL4, biotin anti-IL4, anti-IL5, anti-IL10, biotin anti-IL10. MTT assay as per manufacture instructions (MilliporeSigma).
Anti cd28 pv 1
Manufactured by BioXCell
Anti-CD28 (PV-1) is a monoclonal antibody that binds to the CD28 receptor on T cells. It is used as a laboratory reagent for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 145 2c11, by BioXCell (1 mentions)
Anti ifn γ, by Thermo Fisher Scientific (1 mentions)
Anti il 10, by BioLegend (1 mentions)
Anti il 2, by BD (1 mentions)
Anti il 5, by BioLegend (1 mentions)
Anti il 17, by BioLegend (1 mentions)
Anti tgfβ, by Thermo Fisher Scientific (1 mentions)
Anti il13, by BioLegend (1 mentions)
Biotin anti il 4, by BioLegend (1 mentions)
Biotinanti il 10, by BioLegend (1 mentions)
Anti il 4, by BioLegend (1 mentions)
Mtt assay, by Merck Group (1 mentions)
Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Anti cd45.2 apc, by BD (1 mentions)
Live dead fixable aqua dead cell stain kit, by BD (1 mentions)
Anti crkl sc 319, by Santa Cruz Biotechnology (1 mentions)
Recombinant mouse icam 1 fc, by R&D Systems (1 mentions)
Anti cd3 2c11, by BioXCell (1 mentions)
Anti cd45, by BD (1 mentions)
Anti cd3 fitc, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cd28 pv 1
1
T Cell Cytokine Profiling in IRF5 Knockout Mice
2
T cell Activation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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anti-CD3 (2C11) and anti-CD28 (PV1) were from BioXCell. Anti-CrkL (SC-319) was from Santa Cruz. anti-CD3-FITC was from E-biosciences. Anti-Crk (#610036), Anti-CD8-FITC, anti-CD3, anti-CD45.1, anti-H-2Kb-Pacific Blue, anti-CD45.2-APC and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit were from BD Bioscience. Secondary antibodies conjugated to appropriate fluorophores and AlexaFluor 488-conjugated phalloidin were obtained from Molecular Probes. Luciferin was purchased from Perkin Elmer and Gold Bio. Recombinant mouse ICAM-1-Fc was from R&D Systems.
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