Cd19 isolation kit

Manufactured by Miltenyi Biotec

The CD19+ isolation kit is a laboratory tool for the isolation and enrichment of CD19+ cells from various sample types. It utilizes magnetic bead-based separation technology to selectively capture and isolate the target cell population.

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Lab products found in correlation

Mil 4, by R&D Systems (1 mentions)

Close spelling variants of this product

CD19 Microbeads isolation kit CD19+ B cell isolation kit Mouse CD19+ B cell isolation kit MACS CD19+ B or CD8+ T cell Isolation Kit Isolation kits

2 protocols using cd19 isolation kit

Th2 Differentiation of Naive CD4+ T Cells

Cited 1 time

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Naive sorted ABM TCR-Tg CD4⁺CD25⁻ T cells (Th0) were isolated (CD4⁺CD25⁻ isolation kit, Milteny Biotec, Auburn, CA), and activated for 5 days with 1 μg/ml of plate-bound anti-CD3 and anti-CD28 antibodies (BD Biosciences) in presence of the same number of isolated (CD19⁺ isolation kit, Milteny Biotec) BM12 CD19⁺ B-cells (which are specifically recognized by the ABM TCR-Tg T-cells). Cultures were supplemented with 5 ng/ml mIL-4 (R&D System, Minneapolis, MN) and 1 μg/ml anti-IFN-γ-Ig (BD Bioscience) for Th2 differentiation. T-cells were the harvested and assessed by RT-PCR (40 (link)) for GATA3 expression (Th2 specific marker) to quantify level of differentiation (43 (link)).

Vergani A., Gatti F., Lee K.M., D’Addio F., Tezza S., Chin M., Bassi R., Tian Z., Wu E., Maffi P., Nasr M.B., Kim J.I., Secchi A., Markmann J.F., Rothstein D.M., Turka L.A., Sayegh M.H, & Fiorina P. (2014). TIM4 regulates the anti-islet Th2 alloimmune response. Cell transplantation, 24(8), 1599-1614.

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Cell Culture and Blood Sample Isolation

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The human lymphoma, leukemia and myeloma cell lines used in the study were cultured in RPMI or IMDM media supplemented with 10% fetal bovine serum and 1% Penicillin-Streptomycin (P-S) as described.^{40 (link),41 (link)} A series of ten fresh peripheral blood cells obtained from untreated patients with B-cell lymphoma with peripheral blood dissemination were included in the study. Primary B cells were isolated from human peripheral blood using a CD19⁺ isolation kit (Miltenyi Biotec). To determine cell viability and apoptosis, cells were cultured in IMDM medium with 20% fetal bovine serum and 1% P-S. The research protocol on human subjects was approved by the University of Navarra Institutional Review Board. Informed consent was obtained from patients and healthy donors, in accordance with the Helsinki Declaration.

Celay J., Lozano T., Concepcion A.R., Beltrán E., Rudilla F., García-Barchino M.J., Robles E.F., Rabal O., de Miguel I., Panizo C., Casares N., Oyarzabal J., Prieto J., Medina J.F., Lasarte J.J, & Martínez-Climent J.Á. (2018). Targeting the anion exchanger 2 with specific peptides as a new therapeutic approach in B lymphoid neoplasms. Haematologica, 103(6), 1065-1072.

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