Recombinant human ATX (also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase-2) was purchased from R&D Systems, Inc., Minneapolis, MN, USA. 1-Oleoyl lysophosphatidic acid sodium salt was from Tocris Bioscience (Bristol, UK). 17-β estradiol (estrogen) was from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). PD98059 [the phosphorylated extracellular signal-regulated kinase (p-ERK) inhibitor] was from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). ATX antibody for western blotting was from Abcam (Cambridge, UK; ab77104) and for immunohistochemistry from Santa Cruz Biotechnology (Texas, USA; sc-374222). Antibodies against LPA receptors (LPA1, LPA2, LPA3) were from Abcam (ab166903, ab38322 and ab219267, respectively). Total/phosphorylated extracellular signal-regulated kinases (t/p-ERK) were from Cell Signaling Technology, Inc. Danvers, MA, USA (4695s and 4370s). Antibodies against GAPDH were from Santa Cruz Biotechnology (sc-51907). Crystal violet staining solution was from Tiangen Biotech Co., Ltd. (Beijing, China). Cell Counting kit (CCK)-8 was from Beyotime Institute of Biotechnology, Haimen, China (C0037). Transwell culture plates were from Corning Incorporated (Corning, NY, USA). Lipofectamine 2000™ transfection reagent was from Invitrogen, Thermo Fisher Scientific, Inc.
1 oleoyl lysophosphatidic acid sodium salt
Manufactured by Bio-Techne
Sourced in United Kingdom
1-Oleoyl lysophosphatidic acid sodium salt is a chemical compound used in research and laboratory settings. It is a lysophospholipid that functions as a signaling molecule. The product is supplied as a lyophilized powder for dissolution in appropriate solvents.
Automatically generated - may contain errors
Lab products found in correlation
Transwell culture plate, by Corning (1 mentions)
Pd98059, by Thermo Fisher Scientific (1 mentions)
Ab166903, by Abcam (1 mentions)
Ab38322, by Abcam (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Sc 51907, by Santa Cruz Biotechnology (1 mentions)
Cell counting kit 8, by Beyotime (1 mentions)
Y 27632, by Bio-Techne (1 mentions)
Accutase, by Biozym (1 mentions)
A83 01, by MedChemExpress (1 mentions)
Y 27632, by MedChemExpress (1 mentions)
2 protocols using 1 oleoyl lysophosphatidic acid sodium salt
1
Lysophosphatidic Acid Signaling Mechanisms
2
Blastoid Induction from Naive hPSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Naive hPSCs cultured on MEFs in PXGL medium were pre-treated with PRC2 inhibitor (1 μM UNC1999) for 4 d before blastoid induction. Blastoids were induced19 as follows. Naive hPSCs cultured in untreated or pre-treated conditions were harvested using accutase (Biozym). The cells were resuspended in PXGL medium supplemented with 10 µM Y-27632 (MedChemExpress), seeded onto gelatin-coated plates and incubated at 37 °C for 70 min to deplete the MEFs. The unattached cells were collected, pelleted through centrifugation and resuspended in N2B27 medium containing 10 µM Y-27632 with or without PRC2 inhibitor (aggregation medium), after which 30,000 cells were seeded onto an array of 200-µm microwells inserted into a well of a 96-well plate. Note that microwell arrays comprising microwells were imprinted into 96-well plates77 (link),78 (link). After 24 h, the aggregation medium was replaced with N2B27 medium supplemented with 1 μM PD0325901, 1 μM A83-01 (MedChemExpress, HY-10432), 500 nM 1-oleoyl lysophosphatidic acid sodium salt (Tocris, 3854), 10 ng ml−1 hLIF and 10 µM Y-27632, with DMSO for control or 1 μM UNC1999 for pre-treated samples. The medium was refreshed every 24 h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!