Vs120 virtual microscope

Manufactured by Olympus

Sourced in Japan

The VS120 is a virtual microscope system designed for digital microscopy. It captures high-resolution images and enables virtual slide scanning. The core function of the VS120 is to digitize and display microscopic samples.

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Lab products found in correlation

Vero e6 cell, by American Type Culture Collection (1 mentions) Olyvia 2, by Olympus (1 mentions) Aec substrate kit, by Abcam (1 mentions) Anti sox9, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

VS120 Virtual Slide Microscope VS120–L100 Virtual Slide Microscope VS120 Virtual Slide Microscope System Virtual Slide Microscope (VS120-S6-W, Virtual Slide microscope VS120-SL VS120-L100-W virtual slide microscope VS120 Virtual Slide Scanner microscope VS120 microscope VS120

5 protocols using vs120 virtual microscope

Histological Analysis of Osteochondral Defect Repair

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The samples obtained from osteochondral knee defects and micromass pellets were fixed for 7 days and 24 h in 10% formalin, respectively. After fixation, the specimens were embedded in paraffin and then paraffin sections were deparaffinized, rehydrated, and washed with PBS. The sections were stained with Masson’s trichrome (MT), hematoxylin–eosin (H&E), Safranin O (SafO), and Alcian blue (AB), as previously described^{59 (link)}. The stained sections were observed using a VS 120 virtual microscope (Olympus, Tokyo, Japan). A quantitative analysis was performed using ImageJ v.1.48 (Aspire Software International, Leesburg, VA, USA). Moreover, the O’Drsicoll histological scoring system was used for the histological assessment^{61 (link)}, and the regenerated cartilage was assessed by three blinded experts.

Choi S.M., Lee K.M., Ryu S.B., Park Y.J., Hwang Y.G., Baek D., Choi Y., Park K.H., Park K.D, & Lee J.W. (2018). Enhanced articular cartilage regeneration with SIRT1-activated MSCs using gelatin-based hydrogel. Cell Death & Disease, 9(9), 866.

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ZIKV-GFP Neutralization Assay

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Vero E6 cells purchased from ATCC were seeded at 3 × 10⁴ cells/well in 96-well plates. ZIKV-GFP (1 × 10⁵ TCID₅₀) was incubated with sera (1:10 dilution) from uninfected or ZIKV-infected WT and LILRB4-KO mice (drawn at 15 dpi) for 1 hour at 37°C, then overlaid on top of the Vero E6 cell monolayers. After incubating for 48 hours at 37°C, GFP-positive cells were detected using an Olympus VS-120 Virtual Microscope.

Lee H.N., Manangeeswaran M., Lewkowicz A.P., Engel K., Chowdhury M., Garige M., Eckhaus M.A., Sourbier C., Ireland D.D, & Verthelyi D. (2022). NK cells require immune checkpoint receptor LILRB4/gp49B to control neurotropic Zika virus infections in mice. JCI Insight, 7(3), e151420.

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Histological Analysis of Bone Regeneration

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Repaired bone tissue sections were fixed for 7 days in 10% formalin at room temperature. After fixation, formalin-fixed specimens were embedded in paraffin. The sections were deparaffinized, rehydrated, and washed with PBS and then used to evaluate bone regeneration in the damaged regions. The prepared bone tissue samples were sliced 4 mm-thick and stained with hematoxylin and eosin to observe bone healing or incubated with human anti-vimentin (Santa Cruz Biotechnology), OPN (Abcam), OCN (Abcam), and CD4 (Abcam) antibodies to confirm whether the regenerated bone tissue had a human origin. The stained tissues were observed using a VS120 virtual microscope (Olympus, Tokyo, Japan), and the images were analyzed using OlyVIA 2.5 (Olympus).

Yoon D.S., Choi Y., Lee K.M., Ko E.A., Kim E.J., Park K.H, & Lee J.W. (2023). Downregulation of the RNA-binding protein PUM2 facilitates MSC-driven bone regeneration and prevents OVX-induced bone loss. Journal of Biomedical Science, 30, 26.

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Dehydration and Plastic Resin Embedding

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The collected vessels were processed by the Cardiovascular Pathology Laboratory (CVP) at Texas A&M University according to standard operating procedures for dehydration and infiltration of plastic resin (methyl methacrylate). Briefly, each vessel was placed in a labeled container and dehydrated by gradually increasing the concentration of the immersion fluid from 70% to 100% ethyl alcohol. The dehydrated tissues were then introduced to the plastic resin (EXAKT Technovit 7200 Embedding Media), and using the EXAKT Technologies, Inc. 520 Light Polymerization Unit (Oklahoma City, OK), the plastic resin hardened under yellow and blue light for 6 and 12 h, respectively. Radiographs were obtained of each plastic block with the X-Tek Hawk Micro-CT unit (Amherst, NH) and used to plan and denote cut planes to obtain desired slides^{22 (link)} (Figure 2). Thin sections were cut from each block using the EXAKT 300 diamond saw; each section was then microground to approximately 11–67 μm in thickness using the EXAKT 400CS micro grinder and then polished. The slides were stained with Hematoxylin and Eosin (H&E) and oil-immersion scanned with a 100× objective on the Olympus VS120 Virtual Microscope (Tokyo, Japan); slides were analyzed using the OlyVIA iPad application.

Jessen S.L., Friedemann M.C., Ginn-Hedman A.M., Graul L.M., Jokerst S., Robinson C.B., Landsman T.L., Clubb FJ J.r, & Maitland D.J. (2019). Microscopic Assessment of Healing and Effectiveness of a Foam-Based Peripheral Occlusion Device. ACS biomaterials science & engineering, 6(5), 2588-2599.

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Histological Assessment of Osteoarthritis in Human Cartilage

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Human cartilage tissue sections were fixed in 10% formalin at room temperature for 7 days. After fixation, the formalin-fixed specimens were embedded in paraffin. The paraffin-embedded sections were deparaffinized, rehydrated, and washed with phosphate-buffered saline, and the tissue sections were evaluated for the disruption of cartilage tissue in the human cartilage. The prepared tissue samples were sliced to a thickness of 4 mm and stained using safranin O solution to observe the progression of OA, or were incubated with anti-SOX9 (Santa Cruz Biotechnology) or anti-CCL4 (Santa Cruz Biotechnology) antibodies to detect SOX9 and CCL4 levels. The signals were detected using the AEC substrate kit (Abcam, Cambridge, UK). The stained tissue sections were observed using a VS120 virtual microscope (Olympus, Tokyo, Japan), and images were analyzed using the OlyVIA 2.5 program (Olympus).

Joung S., Yoon D.S., Cho S., Ko E.A., Lee K.M., Park K.H., Lee J.W, & Kim S.H. (2021). Downregulation of MicroRNA-495 Alleviates IL-1β Responses among Chondrocytes by Preventing SOX9 Reduction. Yonsei Medical Journal, 62(7), 650-659.

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