Approximately 48 h after transfection, cells were harvested and the activity of both firefly and Renilla luciferases were measured using a Dual-Luciferase Reporter Assay System (Promega) on a Modulus microplate multimode reader (Turner Biosystems, CA, USA). The normalized luciferase data (firefly/renilla) were used to perform the average statistics of three replicates.
Prl tk renilla luciferase reporter vector
The PRL-TK Renilla luciferase reporter vector is a plasmid that contains the Renilla luciferase gene under the control of a thymidine kinase (TK) promoter. Renilla luciferase is a bioluminescent reporter enzyme that can be used to measure gene expression or other cellular events in a variety of experimental systems.
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54 protocols using prl tk renilla luciferase reporter vector
HEK-293T Cell Transfection Assay
Approximately 48 h after transfection, cells were harvested and the activity of both firefly and Renilla luciferases were measured using a Dual-Luciferase Reporter Assay System (Promega) on a Modulus microplate multimode reader (Turner Biosystems, CA, USA). The normalized luciferase data (firefly/renilla) were used to perform the average statistics of three replicates.
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