Five 4T1-Luc xenograft mice expressing luciferase received an intraperitoneal injection of 150 mg/kg d -luciferin in phosphate buffered saline (Gold Biotechnology, St. Louis, Missouri) for BLI. Mice were then imaged at 10 minutes under isoflurane anesthesia with IVIS 50 (PerkinElmer, Waltham, Massachusetts; Living Image 4.3, 1- or 10-second exposures, bin8, field of view 12 cm, f/stop1 and open filter). The total photon flux (photons/second) was measured from software-defined contour regions of interest over the tumors using Living Image 2.6. Bioluminescence from viable tumor cells was used to estimate tumor burden.
Phosphate buffered saline (pbs)
Manufactured by GoldBio
Phosphate buffered saline (PBS) is a widely used buffer solution in biological research. It is a balanced salt solution that maintains a stable pH and osmolarity, making it suitable for preserving the structure and function of cells and tissues. PBS is typically used for various applications, such as cell culture, immunoassays, and sample preparation.
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4 protocols using phosphate buffered saline (pbs)
1
Bioluminescence Imaging of 4T1-Luc Tumors
2
Bioluminescence Imaging of 4T1-Luc Tumors
3
Bioluminescence Imaging of 4T1-Luc Xenograft Mice
4
Bioluminescence Imaging of Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo, BLI was conducted on a cryogenically cooled IVIS Lumina LT Series using Living Imaging (Caliper Lifesciences). Mice have received an intraperitoneal injection of 150 mg/kg of luciferin potassium salt dissolved in 100 μl PBS (Gold Biotechnology, St. Louis, MO) and subsequently anesthetized with 5% isoflurane (Abbott, North Chicago, IL). The animals were then placed into the BLI chamber and the anesthesia was continually provided with 2% isoflurane by the nose cone. Images were acquired 15 min after luciferin administration. An integration time of 1 min with a binning of 100 pixels was used for luminescence image acquisition. Signal intensity was quantified as total photon flux (p/s/cm2/sr) within the region of interest after subtraction of the background luminescence (22 (link)).
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In vivo, BLI was conducted on a cryogenically cooled IVIS Lumina LT Series using Living Imaging (Caliper Lifesciences). Mice have received an intraperitoneal injection of 150 mg/kg of luciferin potassium salt dissolved in 100 μl PBS (Gold Biotechnology, St. Louis, MO) and subsequently anesthetized with 5% isoflurane (Abbott, North Chicago, IL). The animals were then placed into the BLI chamber and the anesthesia was continually provided with 2% isoflurane by the nose cone. Images were acquired 15 min after luciferin administration. An integration time of 1 min with a binning of 100 pixels was used for luminescence image acquisition. Signal intensity was quantified as total photon flux (p/s/cm2/sr) within the region of interest after subtraction of the background luminescence (22 (link)).
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