Myoglobin rat elisa kit

Serum urea nitrogen (BUN), creatinine (Cr) and creatine kinase (CK) were analyzed by Hitachi 7170 automated biochemistry analyzer (Tokyo, Japan). Serum myoglobin was evaluated by ELISA using a Myoglobin Rat ELISA Kit (Abcam, Cambridge, USA) as follows: samples were diluted 1:3. Then, 100 μl of standards or samples was pipetted into the wells of a microtiter plate. The plate was incubated at room temperature for 60 min. After washing the plate, 100 μl of enzyme-antibody conjugate was pipetted into each well. The plate was then incubated in the dark at room temperature for 30 min. After washing the plate, 100 μl of TMB substrate solution was pipetted into each well. The plate was subsequently incubated in the dark at room temperature for 10 min. After that, 100 μl of stop solution was immediately added to each well. The absorbance (450 nm) of the contents of each well was measured by a microplate reader (Infinite F50, TECAN, Salzburg, Austria). A standard curve was drawn, and a four parameter algorithm equation was established by Magellan software (TECAN). The myoglobin concentration in each sample was then calculated by the software.

Myo content was measured using the Myoglobin Rat ELISA kit (157739, Abcam, US) according to the manufacturer's instructions. Finally, the quantity of Myo in the serum was interpolated from the standard curve plotted from the standards and corrected for sample dilution.