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2 protocols using tg 003

1

Comprehensive Immune Cell Profiling

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Anti-human monoclonal antibodies were used against: CD3 (UCHT1), CD27 (LG.7F9), CD4 (RPA-T4), CD7 (4H9) and panαβTCR (IP26) from eBioscience; CD28 (CD28.2), CD8 (SK1), CD45 (HI30), Vδ2 (B6), CD3 (OKT3), CD45RA (HI100), Annexin-V and 7-AAD from Biolegend; panγδTCR (5A6.E9) from ThermoFisher; Vδ1 (REA173) from Miltenyi Biotech; p-S129-AKT, AKT, p-S9-GSK3β, GSK3β, p-S380-PTEN, PTEN, p-S235/236-S6 and S6 from Cell Signaling; Calnexin and GAPDH from Sicgen; 7-AAD from Invitrogen and Bcl-2 from Dako. Recombinant human sCD27 ligand and recombinant human IL-2 were purchased from Peprotech; CX-4945 (Silmitasertib) from Adooq Bioscience and Biorbyt; TBB from Sigma; TG-003 and Harmine from Focus Biomolecules.
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2

Multicolor Flow Cytometry Immunophenotyping

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Anti-human monoclonal antibodies were used against: CD3 (UCHT1), CD27 (LG.7F9), CD4 (RPA-T4), CD7 (4H9) and panαβTCR (IP26) from eBioscience (San Diego, CA, USA); CD28 (CD28.2), CD8 (SK1), CD45 (HI30), Vδ2 (B6), CD3 (OKT3), CD45RA (HI100), Annexin-V and 7-aminoactinomycin D (7-AAD) from Biolegend (San Diego, CA, USA); panγδTCR (5A6.E9) from ThermoFisher (Rockford, IL, USA); Vδ1 (REA173) from Miltenyi Biotech; p-S129-AKT, AKT, p-S9-GSK3β, GSK3β, p-S380-PTEN, PTEN, p-S235/236-S6 and S6 from Cell Signaling (Danvers, MA, USA); Calnexin and GAPDH from Sicgen (Cantanhede, Portugal); 7-AAD from Invitrogen (Carlsbad, CA, USA) and B-cell lymphoma 2 (Bcl-2) from Dako (Glostrup, Denmark). Recombinant human sCD27 ligand and recombinant human IL-2 were purchased from Peprotech (Rocky Hill, NJ, USA); CX-4945 (Silmitasertib) from Adooq Bioscience (Irvine, CA, USA) and Biorbyt (Cambridge, UK); TBB from Sigma-Aldrich; TG-003 and Harmine from Focus Biomolecules (Plymouth Meeting, PA, USA).
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