Creatinine cr assay kit sarcosine oxidase

Antibodies RIP1, RIP3, KIM-1, Nox2, Nox4, TNF-α, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Rabbit anti-P-MLKL and Anti-cleaved-caspase-3, cleaved-caspase-12 were obtained from Cell Signaling Technology (CST, Danvers, MA, USA). Lipofectamine 3000 was purchased from Science Biotechnology (Invitrogen, Beijing, China). Protein Assay Kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Cell Malondialdehyde (MDA) assay kit (Colorimetric method), reduced glutathione (GSH) assay kit, creatinine (Cr) Assay kit (sarcosine oxidase) and urea (BUN) assay kit were obtained from Nanjing Jiancheng Bioengineering Institute(Nanjing, China). Reactive Oxygen Species Assay (DCF Assay) Kit and Dihydroethidium (DHE) were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). ITC AnnexinV/propidiuiodide was purchased from Bestbio (Shanghai, China). Mouse NGAL ELISA kit was purchased from CUSABIO (Wuhan, China).

Axitinib was synthesized by the Pharmaceutical Design and Synthesis Laboratory of Tianjin University of Science and Technology. Losartan was purchased from Anaiji Chemical Co., Ltd. (Shanghai, China). Trypsin 0.25% solution (Hyclone), RPMI (Roswell Park Memorial Institute) medium 1640 basic (Gibco) and fetal bovine serum were obtained from Solarbio Co., Ltd. (Beijing, China). PBS (Phosphate Buffer Saline, 10×) solution was prepared by NaCl (80.00 g), Na₂HPO₄•12H₂O (29 g), KCl (2 g), KH₂PO₄ (2 g), which was dissolved in 1 L of sterile double distilled water. The formalin solution was made up of 100 mL of 40% formaldehyde solution and 900 mL of 1 × PBS solution. The aspartate aminotransferase assay kit, alanine aminotransferase assay kit, creatinine (Cr) assay kit (sarcosine oxidase), urine protein test kit, endothelin-1 assay kit, and endothelial nitric oxide synthase assay kit were purchased from Nanjing Jiancheng Bioengineering Institute. The 786-O (Human renal clear cell adenocarcinoma) was furnished from Peking Union Medical College. The Wistar rats, C57BL/6N mice, and BALB/c nude mice were purchased from Charles River Laboratories, Beijing, all animal experiments carried out in accordance with the National Institutes of Health guide for the care and use of Laboratory animals and approved by the Academic Committee of Tianjin University of Science and Technology.

Sources of antibodies and reagents are as follows: fetal bovine serum (FBS) (Invitrogen), DMEM/F12 (Invitrogen), anti-cleaved caspase 3 (Cat#: 9661, Cell Signaling Technology), anti-Bcl-2 (Cat#: 12789-1-AP, Proteintech), anti-Bcl-2–associated X (BAX) (Cat#: 50599-2, Proteintech), p-AKT (ser473) (Cat#: 4060P, Cell Signaling Technology), AKT (Cat#: 9272, Cell Signaling Technology), p-ERK1/2 (Cat#: 4370, Cell Signaling Technology), ERK1/2 (Cat#: 4695, Cell Signaling Technology), TRPC6 (Cat#: ACC-017, Alomone), polycystin 2 (Cat#: 19126-1-AP, Proteintech), AURKA (Cat#: 66757-1-lg, Proteintech), HIF-1α (Cat#: 79233, Cell Signaling Technology), p62 (Cat#: 88588, Cell Signaling Technology), β-actin (Cat#: TA-09, ZhongShan Biotechnology), HRP-conjugated anti-rabbit IgG (Cat#: 110777, KeRui Biotechnology), HRP-conjugated anti-mouse IgG (Cat#: 117228, KeRui Biotechnology), U0126 (Cat#: HY-12-31, MedChemExpress), MK2206 (Cat#: HY-10358, MedChemExpress), thapsigargin (Tg) (Cat#: T7459, Invitrogen), JC-1 dye (Bio-Swamp), Creatinine (Cr) Assay Kit (sarcosine oxidase) (Cat#: C011-2, Nanjing Jiancheng), Urea Assay Kit (urease) (Cat#: C013-2, Nanjing Jiancheng), and OCT compound (4583, Sakura). DMEM/F12 and FBS were purchased from Invitrogen (Chicago, California, United States). The whole sagittal section of the kidney was scanned by Biossci Biotechnology Company (Wuhan, Hubei, China).