Anti rip1 610459

Manufactured by BD

Sourced in United States

Anti-RIP1 (610459) is a laboratory reagent used for scientific research purposes. It is an antibody that specifically targets the Receptor-Interacting Serine/Threonine-Protein Kinase 1 (RIP1) protein. The core function of this product is to enable the detection and analysis of RIP1 in various experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using anti rip1 610459

Apoptosis pathway antibody panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

TNF-α and z-VAD-fmk were purchased from R&D. Cyclohexamide (CHX) from Sigma. Smac mimetic was a gift from S. Wang (University of Michigan, Ann Arbor, Michigan, USA). TRAIL from Novoprotein. FasL from Upstate Biotech. CH11 from MBL Medical and Biol Lab.
All antibodies are at concentration of 1 μg ml⁻¹ and used at 1:1,000 dilution unless otherwise stated. Anti-RARγ (C-15) (sc-550) for human, anti-RARα (C-20) (sc-551), anti-caspase-8 (C-20) (sc-6136), anti-cIAP2 (sc7944) and anti-Fas (C-20) (sc-715) from Santa Cruz; anti-RIP1 (610459) and anti-FADD (610400) from BD Biosciences; anti-RARγ1 (ab5905) for mouse, anti-RIP3 (ab72106), anti-MLKL (ab184718) for human, anti-MLKL (ab172868) for mouse and anti-cIAP1 (ab2399) from Abcam; anti-RIP3 (2283) for mouse from ProSci, anti-TRADD (05-473) from Upstate; anti-TRAF2 (MAB3277) and anti-TNFR1 (AF-425-PB) from R&D; anti-Actin (A3853) (dilution 1:10,000), anti-FLAG (F9291) (dilution 1:5,000) and anti-GFP (G6539) (dilution 1:5 000) from Sigma; anti-V5 (R960-25) (dilution 1:5,000) from Invitrogen; anti-PARP1 (BML-SA250-0050) from Enzo Life Science; anti-GAPDH (NB300-22) (dilution 1:5,000) from Novus Biologicals; anti-cleaved caspase-8 (9496), anti-CYLD (4495), anti-RIP1 (137451) and anti-p-RIP1(65746) from Cell Signaling Technology; anti-DsRed (632392) (dilution 1:5,000) from Clontech.

Xu Q., Jitkaew S., Choksi S., Kadigamuwa C., Qu J., Choe M., Jang J., Liu C, & Liu Z.G. (2017). The cytoplasmic nuclear receptor RARγ controls RIP1 initiated cell death when cIAP activity is inhibited. Nature Communications, 8, 425.

+ Open protocol

+ Expand

TNF-induced cell death pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Z-VAD and Smac mimetic were used as described previously (Wang H. et al., 2014 (link)). Recombinant TNF was purified in our laboratory, AP20187 (HY-13992) was purchased from MCE. ICP6 polyclonal antibodies can be obtained by injecting His-ICP6 (1-177 amino acids) expressed in E. coli into rabbits. The following antibodies were used in this study: anti-Flag M2 (F3165), anti-Myc (SAB4700448) (Sigma); anti-RIP1 (610459) (BD Biosciences); anti-RIP1 (D94C12), anti-phospho-RIP1 (65746S) and anti-hRIP3 (13526S) (Cell Signaling Technology); anti-FKBP (ab108420) (Abcam); and anti-actin (PM053-7) (MBL).

Hu H., Wu G., Shu Z., Yu D., Nan N., Yuan F., Liu X, & Wang H. (2020). ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling. Frontiers in Cell and Developmental Biology, 8, 595253.

+ Open protocol

+ Expand

Western Blotting for Apoptosis Signaling Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting followed by enhanced chemiluminescence detection was performed as previously described.^{24 (link)} In some experiments, cell culture supernatants were also collected for the detection of HMGB1 or GAPDH. The following antibodies were used: Anti-TAK1 (4505), anti-phospho-TAK1 (Thr187; 4536), anti-α tubulin (3873), anti-HMGB1 (3935), anti-PARP (9532), anti-caspase 8 (4790), anti-cleaved caspase 8 (9429), anti-caspase 3 (9662), anti-RIP1 (3493), anti-CYLD (8462), anti-TRADD (3694), anti-cIAP1 (4952), and anti-FLIP (8510) were from Cell Signaling Biotechnology (Beverly, MA, USA); anti-RIP3 (sc-135171), anti-FADD (sc-6036), anti-CYLD (sc-74435), anti-IκBα (sc-847), anti-p65 (sc-372), anti-cIAP2 (sc-7944), and anti- GAPDH antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-RIP1 (610459) was from BD Biosciences; anti-FLIP (XA-1008) was from ProSci (Poway, CA, USA); anti-FADD (AD-I-AAM-212-E) was from Enzo Life Sciences (Farmingdale, NY, USA).

Guo X., Yin H., Chen Y., Li L., Li J, & Liu Q. (2016). TAK1 regulates caspase 8 activation and necroptotic signaling via multiple cell death checkpoints. Cell Death & Disease, 7(9), e2381-.

+ Open protocol

+ Expand

Antibody Characterization for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-mRip3, p-Rip3, Fadd and Mlkl antibodies were described previously^{19 (link)}. Anti-Ppm1b antibody was raised in rabbit using GST-tagged full-length human Ppm1b-L as the antigen. Anti-human Rip3 (ab72106, dilution 1:1,000) and anti-Ppm1a (ab14824, dilution 1:1,000) antibodies were from Abcam. Anti-Flag (F7425, dilution 1:500), Myc (A7470, agarose affinity gel) and HA (A2095, agarose affinity gel) antibodies were obtained from Sigma. Anti-Myc (sc-40, dilution 1:500), HA (sc-805, dilution 1:500) and anti-β-actin (sc-47778, dilution 1:10,000) antibodies were obtained from Santa Cruz Biotechnology. Anti-Rip1 (610459, dilution 1:1,000) antibody was obtained from BD Biosciences. Anti-Gapdh (60004-1-Ig, dilution 1:2,000) and anti-Canx (10427-2-AP, dilution 1:500) antibodies were from Proteintech. Anti-p-p65 (3033s, dilution 1:1,000), p65 (8242s, dilution 1:200), p-p38 (9216S, dilution 1:1,000), p38 (9212s, dilution 1:200) and H3 (9715, 1:5,000) antibodies were obtained from Cell Signaling Technology. Mouse TNFoc was purchased from eBioscience. zVAD was obtained from Calbiochem. Propidium iodide (PI), 4-hydroxytamoxifen (4-OHT) and Protease Inhibitor Mix were from Sigma. λ-phosphatase (λ-Ppase) was from NEB. AP21967 (A/C Heterodimerizer) was obtained from ARIAD Pharmaceuticals.

Chen W., Wu J., Li L., Zhang Z., Ren J., Liang Y., Chen F., Yang C., Zhou Z., Su S.S., Zheng X., Zhang Z., Zhong C.Q., Wan H., Xiao M., Lin X., Feng X.H, & Han J. (2015). Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. Nature cell biology, 17(4), 434-444.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!