The human primary hepatocytes were provided by BioreclamationIVT (F00995-P and M00995-P, Hicksville, New York, USA), and the clinical characteristics of the donors are shown in Supplementary Table 1 . Human primary hepatocytes were thawed in INVITROGROTM CP Medium (S03316, BioreclamationIVT, Hicksville, New York, USA) supplemented with 10% fetal bovine serum (16140071, Gibco, New York, USA) at 37 °C in a 5% CO2 incubator for 24 h and then replaced with INVITROTM HI Medium (Z99009, Hicksville, New York, USA). HL-7702 (JNO-048) was purchased from GuangZhou Jennio Biotech Co.,Ltd. (Guangzhou, Guangdong, China), SW-480 (TCHu 86), HCT-116 (TCHu 99), Bel-7402 (TCHu 10), Hep-G2 (TCHu 72), and HEK-293T (GNHu 18) cells were originally obtained from the Cell Bank of China Science Academy. HL-7702, SW-480, HCT-116 and Bel-7402 cells were maintained in RPMI-1640 (21870076, Gibco, New York, USA), while HEK-293T and Hep-G2 cells were maintained in DMEM (10569010, Gibco, New York, USA) with 10% fetal bovine serum (16140071, Gibco, New York, USA), 100 U/mL penicillin and 100 μg/mL streptomycin (15140122, Gibco, New York, USA) in a humidified atmosphere with 5% CO2. All cell lines were routinely tested to be negative for mycoplasma contamination.
Bel 7402
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The Bel-7402 is a laboratory equipment designed for general laboratory use. It is a precision instrument that can perform a variety of tasks within a controlled environment. The core function of the Bel-7402 is to provide accurate and reliable data for scientific analysis and research.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Thermo Fisher Scientific (34 mentions)
Smmc 7721, by Thermo Fisher Scientific (34 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (30 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (28 mentions)
Hep3b, by Thermo Fisher Scientific (16 mentions)
Hepg2, by American Type Culture Collection (15 mentions)
Rpmi 1640, by Thermo Fisher Scientific (14 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (12 mentions)
Bel 7402, by American Type Culture Collection (11 mentions)
Smmc 7721, by American Type Culture Collection (10 mentions)
Hl 7702, by Thermo Fisher Scientific (8 mentions)
Hep3b, by American Type Culture Collection (8 mentions)
Mhcc97h, by Thermo Fisher Scientific (7 mentions)
Dmem medium, by Thermo Fisher Scientific (7 mentions)
Sk hep1, by Thermo Fisher Scientific (7 mentions)
Streptomycin, by Thermo Fisher Scientific (6 mentions)
Penicillin, by Thermo Fisher Scientific (6 mentions)
Mhcc97l, by Thermo Fisher Scientific (6 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (6 mentions)
Hek293t, by Thermo Fisher Scientific (5 mentions)
67 protocols using bel 7402
1
Isolation and Culture of Human Hepatocytes
2
Demethylation Assay for HCC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HCC cell lines (Bel-7402, HepG2, Huh7, MHCC-97H, MHCC-97L, SMMC-7721, SNU-387, and SNU-449) and immortalized normal liver cell line LO2 were purchased from American Type Culture Collection and Cell Resource Center of Shanghai Institutes. HepG2, Huh7, MHCC-97H, MHCC-97L, and LO2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS (BI). Bel-7402, SMMC-7721, SNU-387, and SNU-449 were cultured in RPMI1640 medium (Gibco) supplemented with 10% FBS (BI). For demethylation treatment, HCC cells were treated with 10μM decitabine (Selleck, S1200) for 3 days and harvested for the following experiments.
3
Cell Line Cultivation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HL-7702, Huh7, BEL-7402, SNU449, and SK-Hep1 cell lines were purchased from the Cell Bank of Type Culture Collection (Chinese Academy of Sciences, Shanghai, China). HL-7702, BEL-7402, Huh7, and SK-Hep1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), and SNU449 cells were cultured in RPIM1640 medium (Gibco). Cells were incubated with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) at 37°C in a humidified atmosphere containing 5% CO2.
4
Cell Culture of Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sensitive cell line Bel7402 and the resistant cell line Bel/5-FU were purchased from Key GENE Biotech, Nanjing, Jiangsu, China. Bel7402 and Bel/5-FU cells were cultured in RPMI-1640 medium (Gibco, CA, USA) supplemented with 10%fetal bovine serum (FBS; Biological Industries, CA, USA), 100 IU/ml penicillin and 100 μg/ml streptomycin (HyClone, MA, USA) in humidified atmosphere with 5%CO2 at 37 °C. 5-FU was added at a concentration of 20,000 ng/mL to the medium of Bel/5-FU cells.
HEK-293T, SMCC-7721, HepG2, Hep3B, and MHCC97H cell lines were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China, and was cultured in DMEM medium (Gibico, CA, USA), supplemented with 10% fetal bovine serum (FBS; Biological Industries, CA, USA), 100 IU/ml penicillin and 100 μg/ml streptomycin (Hyclone, MA, USA) in humidified 5% CO2 at 37 °C.
HEK-293T, SMCC-7721, HepG2, Hep3B, and MHCC97H cell lines were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China, and was cultured in DMEM medium (Gibico, CA, USA), supplemented with 10% fetal bovine serum (FBS; Biological Industries, CA, USA), 100 IU/ml penicillin and 100 μg/ml streptomycin (Hyclone, MA, USA) in humidified 5% CO2 at 37 °C.
5
Culturing Hepatocellular Carcinoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hepatocellular carcinoma cell lines, including Bel-7402 (catalogue number TCHu 10), QGY-7703 (catalogue number TCHu 43), MHCC97L (catalogue number CC0109), MHCC97H (catalogue number SCSP 528) were cultured in RPMI 1640 (Gibco BRL, Rockville, MD) supplemented with 10% fetal bovine serum (Gibco BRL, Rockville, MD), penicillin (100 units/ml) and streptomycin (100 units/ml), and maintained in a 5% CO2-humidified incubator at 37 °C. All cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).
6
Culturing Human Liver Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines SNU423, Hep3B, and Bel-7402 were obtained from China Type Culture Collection (Shanghai, China). SNU423 and Bel-7402 cells were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum and 100 U/mL penicillin/streptomycin at 37°C under 5% CO2. The Hep3B cells were cultured in complete MEM (Gibco) under the same conditions.
7
Hypoxia Induction in Human Liver Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines HepG2, SMMC-7721 and Bel-7402 were purchased from Shanghai Institutes for Biological Sciences (Chinese Academy of Sciences, Shanghai, China). Bel-7402 and SMMC-7721 were normally cultured in RPMI1640 medium (Gibco, Grand Island, NY, USA), all supplement with 10% FBS (Gibco, Grand Island, NY, USA) in a 5% CO2 humidified incubator at 37 degrees. And in order to get hypoxic cells. Cells were exposed to hypoxic conditions (1% O2) in a hypoxia incubator filled with a mixture of 1% O2, 5% CO2and 94% N2.
8
Cell Culture Protocols for Cancer and Normal Liver Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All cell lines (Bel-7402, Huh7, and L02) were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai Institute of Cell Biology). Bel-7402 and L02 cells were cultured in 1640 medium (Gibco, Australia), and Huh7 cells were cultured in DMEM medium (Gibco). All cells were cultured with 10% fetal calf serum (Gibco), and 1% penicillin/streptomycin (Gibco). All cells were maintained in a humidified atmosphere containing 5% CO2 at 37°C.
9
Cultivation of Human Liver Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines (Huh7, Bel-7402, SMMC-7721, HepG2, and SNU449) and the liver cell line LO2 were purchased from ATCC and incubated at a humidified incubator (condition: 37°C, 5% CO2). All the HCC cells were grown in DMEM medium (Gibco), except Bel-7402 and SNU49 cells (RPMI 1640; Gibco), which contained 10% FBS (Gibco) and 1% penicillin/streptomycin (Sigma).
10
Hypoxia-Reoxygenation Injury in Liver Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell line (bel-7402 and Huh7), mouse HCC cell line (Hepa1–6) and mouse normal cell line (AML12) were purchased from Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai Institute of Cell Biology). Huh7 and Hepa1–6 were maintained in Dulbecco’s modied Eagle’s medium (DMEM) supplemented with 10% FBS (Gibco, USA) at 37 °C and 5% CO2, and the bel-7402 cell was cultured in RPMI-1640 medium (Gibco, USA). AML12 cell was maintained in DMEM/Ham’s F-12 Nutrient Mixture supplemented with 10% FBS (Gibco, USA) at 37 °C and 5% CO2. Cell lines were authenticated by short tandem repeats (STR) profiling and confirmed to be mycoplasma negative.
To evaluate the hypoxia-reoxygenation (H/R) injury in vitro, AML12 cells were treated with palmitic acid (PA, Sigma-Aldrich, USA) for 24 h followed by fluxed with 95% N2/5% CO2 without FBS and maintained at 37 °C for 16 h. For reoxygenation, cells were transferred to a 95% air/5% CO2 gas mixture with 10% FBS for 2 h.
To evaluate the hypoxia-reoxygenation (H/R) injury in vitro, AML12 cells were treated with palmitic acid (PA, Sigma-Aldrich, USA) for 24 h followed by fluxed with 95% N2/5% CO2 without FBS and maintained at 37 °C for 16 h. For reoxygenation, cells were transferred to a 95% air/5% CO2 gas mixture with 10% FBS for 2 h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!