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Rabbit monoclonal anti tgfβ

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Rabbit monoclonal anti-TGFβ is a primary antibody that specifically binds to the Transforming Growth Factor Beta (TGFβ) protein. It is produced in rabbit and is designed for research applications.

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Lab products found in correlation

Rabbit monoclonal anti gapdh, by Abcam (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Odyssey v3.0 scanner, by LI COR (1 mentions) Mouse monoclonal anti runx2, by Abcam (1 mentions) Rabbit monoclonal anti ppar γ, by Cell Signaling Technology (1 mentions) Rabbit monoclonal anti il 6, by Cell Signaling Technology (1 mentions) Rabbit polyclonal anti mmp 9, by Abcam (1 mentions)

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Rabbit anti- (5 citations)

2 protocols using rabbit monoclonal anti tgfβ

1

Western Blot Analysis of Osteogenic Markers

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The total protein was extracted from the cultured cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) and transferred onto a PVDF membrane (Bio-rad, United States). The membrane was incubated with rabbit monoclonal anti-BMP2 (1:1000; Proteintech, United States), rabbit monoclonal anti-BMP4 (1:1000; Affinity Biosciences, United States), rabbit monoclonal anti-DMP1 (1:1000; Abcam, United Kingdom), rabbit monoclonal anti-ALP (1:1000; Abcam, United Kingdom), mouse monoclonal anti-RUNX2 (1:1000; Abcam, United Kingdom), rabbit monoclonal anti-TGFβ (1:1000; Abcam, United Kingdom), rabbit monoclonal anti-DSPP (1:1000; Affinity Biosciences, United States), rabbit monoclonal anti-MSX2 (1:1000; Bioss, CHN), and rabbit monoclonal anti-GAPDH (1:1000; Abcam, United Kingdom) overnight at 4°C. Proteins were visualized using HRP-conjugated donkey anti-rabbit IgG (1:20000; BBI Life Sciences) and HRP-conjugated donkey anti-mouse IgG (1:20000; BBI Life Sciences) secondary antibody. The membrane was scanned on an Odyssey V3.0 scanner (Li-cor).
Li X., Xu W., Lin X., Wu J, & Wu B. (2022). Effect of LncRNA-MALAT1 on mineralization of dental pulp cells in a high-glucose microenvironment. Frontiers in Cell and Developmental Biology, 10, 921364.
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2

Comprehensive Protein Analysis Protocol

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Protein was extracted by RIPA buffer containing protease and phosphatase inhibitors. 6-10% Bis-Tris protein gels were equally loaded with 30μg protein, electrophoresed at 110 V, and electro-transferred to PVDF membranes. Membranes were blocked in 10% milk/TBST and immunoblotted with the following antibodies: rabbit polyclonal anti-MMP-9 (Abcam, 1:1000), rabbit monoclonal anti-TGF-β (Abcam, 1:1000), rabbit polyclonal anti-ARG1 (Abcam, 1:1000), rabbit monoclonal anti-VEGF-A (Abcam, 1:1000), rabbit monoclonal anti-S100A8 (Abcam, 1:1000), rabbit monoclonal anti-TNF-α (Abcam, 1:1000), rabbit monoclonal anti-IL-6 (Cell Signaling Technology, 1:1000), rabbit polyclonal anti-DNMT1 (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-EGR1(Abcam, 1:1000), rabbit monoclonal anti-EGR2 (Abcam, 1:1000), rabbit monoclonal anti-PPAR-γ (Cell Signaling Technology, 1:1000), mouse monoclonal anti-Maf-b (Santa Cruz Biotechnology, 1:100), rabbit monoclonal anti-p50 (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-p52 (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-Rel-B (Cell Signaling Technology, 1:1000), rabbit monoclonal anti-p65 (Cell Signaling Technology, 1:1000), mouse monoclonal antî-Actin (Sigma Aldrich, 1:10000). The loading control antibodies (ant-β-Actin) in all cases were applied. Information about all the antibodies used is provided in Supplementary Table 3.
Lu Z., Zou J., Li S., Topper M.J., Tao Y., Zhang H., Jiao X., Xie W., Kong X., Vaz M., Li H., Cai Y., Xia L., Huang P., Rodgers K., Lee B., Riemer J.B., Day C.P., Yen R.W., Cui Y., Wang Y., Wang Y., Zhang W., Easwaran H., Hulbert A., Kim K., Juergens R.A., Yang S.C., Battafarano R.J., Bush E.L., Broderick S.R., Cattaneo S.M., Brahmer J.R., Rudin C.M., Wrangle J., Mei Y., Kim Y.J., Zhang B., Wang K.K., Forde P.M., Margolick J.B., Nelkin B.D., Zahnow C.A., Pardoll D.M., Housseau F., Baylin S.B., Shen L, & Brock M.V. (2020). Epigenetic Therapy Inhibits Metastases by Disrupting Premetastatic Niches. Nature, 579(7798), 284-290.
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