Formvar coated 200 mesh copper grid

Manufactured by Ted Pella

Sourced in United States

The Formvar-coated 200-mesh copper grid is a specimen support for transmission electron microscopy (TEM) applications. It consists of a copper mesh grid coated with a thin layer of Formvar, a plastic material that provides a stable and continuous support film for the sample.

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Lab products found in correlation

Uv 2401 spectrometer, by Shimadzu (3 mentions) Jem 2100 plus, by JEOL (2 mentions) Tecnai g2 spirit twin transmission, by Thermo Fisher Scientific (2 mentions) Magellan 400l xhr sem, by Thermo Fisher Scientific (1 mentions) Tecnai g2 f30 s twin, by Thermo Fisher Scientific (1 mentions) Sem feg gemini 500, by Zeiss (1 mentions) Tecnai g2 spirit twin transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Tecnai t12 tem, by Thermo Fisher Scientific (1 mentions) Gatan ultra scan ccd camera, by Ametek (1 mentions) Jem 1400, by JEOL (1 mentions)

10 protocols using formvar coated 200 mesh copper grid

Nanoparticle Size Characterization by TEM

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The NP suspension was deposited onto a formvar-coated 200-mesh copper grid (Ted Pella, Redding, CA, USA) and allowed to dry before examination. NPs were then visualized with a Jeol JEM 2100Plus (Jeol, Tokyo, Japan) electron microscope equipped with a 9 MP complementary metal oxide superconductor (CMOS) Gatan Rio9 digital camera (Gatan Inc., Pleasanton, CA, USA). The core diameter (n = 100) was calculated by processing the recorded images with Fiji/ImageJ software [24 (link)].

Salvioni L., Testa F., Barbieri L., Giustra M., Bertolini J.A., Tomaino G., Tortora P., Prosperi D, & Colombo M. (2022). Saporin Toxin Delivered by Engineered Colloidal Nanoparticles Is Strongly Effective against Cancer Cells. Pharmaceutics, 14(7), 1517.

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Visualizing Gold Nanoparticle-Antibody Complexes

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Transmission Electron Microscopy. Gold nanoparticles and Gold nanoparticles – antibody complexes were visualized using FEI MAGELLAN 400 L XHR SEM. This microscope allows for images acquisition in the high energy (15–30 kV) SEM and STEM mode. The ultrathin Formvar-coated 200-mesh copper grid (Ted-pella, Inc.) were directly dipped in the sample solution and left to dry in air overnight. TEM images of the prepared colloidal AuNP were used for the size distribution measurements. For each sample, the size of at least 100 particles was measured and the average size and the standard distribution were obtained. TEM and SEM images of the prepared AuNP – antibody complexes were used for the analysis of surface contact between AuNPs in the case of total antibody surface passivation or partial antibody surface passivation. The charging effect observable on the edge of the NPs visualized by SEM is associated to the presence of the organic layer. This effect provides information on the degree of NP surface passivation by the antibody.

Astorga-Gamaza A., Vitali M., Borrajo M.L., Suárez-López R., Jaime C., Bastus N., Serra-Peinado C., Luque-Ballesteros L., Blanch-Lombarte O., Prado J.G., Lorente J., Pumarola F., Pellicer M., Falcó V., Genescà M., Puntes V, & Buzon M.J. (2020). Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells. Nano today, 36, 101056.

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Nanoparticle Characterization by TEM

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NPs were visualized using an FEI 120 KV Tecnai G2 spirit Bio TWIN Instrument. The sample (2 µL) was deposited onto a formvar-coated 200-mesh copper grid (Ted Pella, Redding, CA, USA), and allowed to dry in air before examination. For the mean diameter determination of NPs, images were processed with ImageJ software. The reported value was calculated measuring at least 100 particles.

Salvioni L., Galbiati E., Collico V., Alessio G., Avvakumova S., Corsi F., Tortora P., Prosperi D, & Colombo M. (2017). Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations. International Journal of Nanomedicine, 12, 2517-2530.

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Imaging GNP-6 Nanoparticles by TEM

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GNP-6 was suspended in DMSO. Ten microliter droplets of the sample were drop casted onto a piece of ultrathin Formvar-coated 200-mesh copper grid (Ted-pella, Inc.) and left to dry in air. Transmission electron microscopy (TEM) images were acquired on FEI Tecnai G2 F30 S-TWIN.

Tu X., Tan X., Qi X., Huang A., Ling F, & Wang G. (2020). Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites. Journal of Nanobiotechnology, 18, 32.

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PLGA-PEG-RhB-NPs Structural Analysis

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PLGA-PEG-RhB-NPs were observed by a Zeiss SEM-FEG Gemini 500, operating at 30 kV in scanning transmission electron microscopy (STEM) mode (Zeiss, Germany). The NPs suspension was deposited onto a formvar-coated 200-mesh copper grid (Ted Pella, CA, USA), negatively stained with 1% uranyl acetate and allowed to dry before examination.

Morelli L., Gimondi S., Sevieri M., Salvioni L., Guizzetti M., Colzani B., Palugan L., Foppoli A., Talamini L., Morosi L., Zucchetti M., Violatto M.B., Russo L., Salmona M., Prosperi D., Colombo M, & Bigini P. (2019). Monitoring the Fate of Orally Administered PLGA Nanoformulation for Local Delivery of Therapeutic Drugs. Pharmaceutics, 11(12), 658.

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Visualizing SARS-CoV-2 VLP Morphology

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VLP morphology was analyzed by transmission electron microscopy (TEM). Briefly, 10 μl of VLP preparation was adsorbed onto Formvar-coated 200-mesh copper grids (Ted Pella, Inc.) for 2 min and then negatively stained with 2 % uranyl acetate for 2 min. Samples were observed in a transmission electron microscope (JEM-2100 plus, Jeol).
For gold-immunolabeling, after VLP adsorption, the grids were blocked with PBS-BSA 1% for 30 min and then incubated for 60 min with an anti-SARS-CoV-2 RBD mAb (NN68) diluted 1/20 dilution in PBS-BSA 0.05%. After a washing step, the grid was incubated with a secondary 10-nm gold-conjugated anti-mouse IgG antibody (G7777, Sigma-Aldrich) diluted 1/20 in PBS-BSA 1% for 60 min. A final wash was performed, and the grids were negatively stained with 2% uranyl acetate for 2 min before TEM observation. All incubations were done in a humid chamber. Electron micrographs were analyzed and cVLP size was measured using ImageJ software (Schindelin et al. 2012 (link)).

Garay E., Fontana D., Villarraza J., Fuselli A., Gugliotta A., Antuña S., Tardivo B., Rodríguez M.C., Gastaldi V., Battagliotti J.M., Alvarez D., Castro E., Cassataro J., Ceaglio N, & Prieto C. (2023). Design and characterization of chimeric Rabies-SARS-CoV-2 virus-like particles for vaccine purposes. Applied Microbiology and Biotechnology, 107(11), 3495-3508.

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Extinction Spectra and TEM Imaging

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Extinction spectra for the GNS and SERS tags were recorded on a Shimadzu UV-2401 spectrometer. Transmission electron micrographs (TEM) were acquired using the FEI Tecnai G2 Spirit TWIN transmission electron microscope at an accelerating voltage of 120 kV. The sample was dropped onto ultrathin Formvar-coated 200-mesh copper grids (Ted Pella, Inc.) and left to dry in air.

Li M., Kang J.W., Sukumar S., Dasari R.R, & Barman I. (2015). Multiplexed detection of serological cancer markers with plasmon-enhanced Raman spectro-immunoassay †Electronic supplementary information (ESI) available: Fig. S1–S7 and Table S1. See DOI: 10.1039/c5sc01054c Click here for additional data file.. Chemical Science, 6(7), 3906-3914.

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Extinction Spectra and TEM Imaging

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Characterization of SERS Agents for Cell Imaging

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Extinction spectra for the GNS and SERS tags were recorded on a Shimadzu UV-2401 spectrometer. Transmission electron micrographs were acquired using the FEI Tecnai G2 Spirit TWIN transmission electron microscope (TEM) at an accelerating voltage of 120 kV. The samples for TEM were prepared by deposition of a drop of the suspensions in ethanol onto ultrathin Formvar-coated 200 mesh copper grids (Ted Pella, Inc.) and left to dry in air. Cell samples for TEM imaging were prepared according to the procedure reported in the literature,⁴² after incubation as described above. Briefly, after incubation with the SERS agents, cells were fixed in a 0.1 M sodium cacodylate buffer solution (pH 7.4) containing 3.0% formaldehyde, 1.5% glutaraldehyde, 5.0 mM CaCl₂ and 2.5% sucrose for 1 h, rinsed with 0.1 M sodium cacodylate buffer solution (pH 7.4) of 2.5% sucrose, and then post-fixed for 1 h in Palade's buffered solution of 1% osmium tetroxide. After dehydration with graded series of cold ethanol (70, 90 and 100%), the samples were washed three times with fresh 100% ethanol, and then twice with propylene oxide at room temperature before being transferred to fresh 100% Epon and embedded in fresh 100% Epon under vacuum for 4–6 h. After being kept at 60 °C for 24–48 h, serial sections were cut and mounted onto copper-grids for examination by TEM.

Li M., Banerjee S.R., Zheng C., Pomper M.G, & Barman I. (2016). Ultrahigh affinity Raman probe for targeted live cell imaging of prostate cancer †Electronic supplementary information (ESI) available: Fig. S1–S7. See DOI: 10.1039/c6sc01739h Click here for additional data file.. Chemical Science, 7(11), 6779-6785.

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Transmission Electron Microscopy Imaging

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TEM samples were prepared on Formvar coated 200 mesh copper grids (Ted Pella Inc., Redding, CA). A total of 10 μL of assembly solution was incubated for 2 min on the grid. Solution was wicked away and the grid incubated on a droplet of 2% methylamine tungstate (Nano-W, Nanoprobes Yaphank, NY) for 10 s, prior to liquid removal and rinsing for 5 s on a droplet of 0.02 μm filtered distilled water. Ambient temperature TEM was performed using a Tecnai T12 TEM (FEI Company, Hillsboro, OR) or JEOL JEM1400 (JEOL USA Inc., Pleasanton, CA) operating at 120 keV. Data was recorded with a 4 × 4 Gatan Ultra Scan CCD camera (Gatan, Pleasanton, CA).

Engelberth S.A., Barino M.S., Sandhu S., Li W., Bonde J, & Habelitz S. (2018). Progression of Self-Assembly of Amelogenin Protein Supramolecular Structures in Simulated Enamel Fluid. Biomacromolecules, 19(10), 3917-3924.

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