Tubp gal80ts

Manufactured by Bloomington Drosophila Stock Center

Sourced in United States

The TubP-GAL80ts is a genetic tool used in Drosophila research. It functions as a temperature-sensitive repressor of the GAL4 transcriptional activator, allowing for temporal control of gene expression in Drosophila. The core function of this tool is to provide researchers with the ability to regulate the timing and level of target gene expression in Drosophila.

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Gal80ts (4 citations) TubP-GAL80ts/TM2 (2 citations)

9 protocols using tubp gal80ts

Rearing Drosophila Flies for Genetic Studies

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Flies were reared on cornmeal/molasses/agar medium under standard culture conditions (29°C, 25°C or 18°C depending on the presence of tubP-Gal80^ts, 12 h:12 h light/dark cycle). CO₂ was used as an anesthetic. slgA^NP4104, UAS-mCD8-gfp, UAS-mito-tomato, UAS-RFP, CkIIα^JF01436, CkIIα ^GL0003, slgA^GL01514, TubP-Gal80^ts, TubP-Gal4, OK107-Gal4 and 201y-Gal4 were obtained from the Bloomington Drosophila Stock Center, Bloomington, IN, USA. P{cry-Gal4.E39} and P{pdf-Gal4.P2.4} were a gift of Dr Bassem Hassan (ICM, Paris, France). All fly stocks were isogenized by mating females to Canton-S males for ten generations to exclude effects due to differences in genetic background. The RNAi lines used were predicted to have no off-target effects (Ni et al., 2009 (link)).

Zwarts L., Vulsteke V., Buhl E., Hodge J.J, & Callaerts P. (2017). SlgA, encoded by the homolog of the human schizophrenia-associated gene PRODH, acts in clock neurons to regulate Drosophila aggression. Disease Models & Mechanisms, 10(6), 705-716.

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Drosophila Tau Transgenic Lines

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GMR-GAL4, elav-GAL4, actin-GAL4, tubulin-Gal4, tau^HM05101, tubP-GAL80^ts, UAS-Dcr-2, tau^Df(3R)MR22, Df(3R)BSC499, and UAS-hTau23 (UAS-MAPT.A59A) were obtained from the Bloomington Stock Center. UAS-dTau::GFP and tau^EP3597 were a gift of D. St. Johnston (University of Cambridge, Great Britain). Appl-Gal4 was obtained from Laura Torroja (Universidad Autonoma de Madrid, Spain). tau^GD25023 was received from the Vienna Drosophila RNAi Center (Vienna, Austria). olk¹ mutants are described in (Bettencourt da Cruz et al., 2005 (link)). Flies were raised under standard conditions at 25°C unless specifically described. To create the MAP60 and MAP205-expressing flies, we used LD02709 for MAP60 and LD12965, which were cloned into pUAST. Transgenic flies were generated by P-element transformation using Best Gene Inc. (Chino Hills, CA).

Bolkan B.J, & Kretzschmar D. (2014). Loss of Tau results in defects in photoreceptor development and progressive neuronal degeneration in Drosophila. Developmental neurobiology, 74(12), 1210-1225.

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Drosophila Rearing and Genetic Stocks

Cited 2 times

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Fly stocks were reared on standard cornmeal media (per 1L H₂0: 12g agar, 29g Red Star yeast, 71g cornmeal, 92g molasses, 16mL methyl paraben 10% in EtOH, 10mL propionic acid 50% in H₂0) at 25°C with 60% relative humidity and entrained to a daily 12hr light, 12hr dark schedule. Canton-S flies were from Gero Miesenböck (University of Oxford), w¹¹¹⁸ flies were from David Krantz (UCLA), LexAOP-CD8::GFP; UAS-flp, PBac{y[+mDint2]w[+mc]=brp(FRT.Stop)V5–2A-LexA-VP16} [52 (link)] was from Lawrence Zipursky (UCLA), yw,per⁰¹ [39 (link)] and yw; tim⁰¹ [38 (link)] were supplied by Amita Sehgal (University of Pennsylvania), and UAS-hiw^ΔRING [59 (link)] was from Aaron Diantonio (Washington University in St. Louis). UAS-hiw^RNAi [87 (link)] was ordered from the Vienna Drosophila Resource Center. w⁺, per⁰¹ [39 (link)], w⁺;tim⁰¹ [38 (link)], cyc⁰¹ [37 (link)], Clk^JRK [36 (link)], OR22a-GAL4 [20 (link)], UAS-kir2.1::GFP [44 (link)], tubP-GAL80^TS [46 (link)], UAS-CD8::GFP [88 (link),89 (link)], para^sbl−1 and para^sbl−2 [47 (link)], orco-GAL4 [45 (link)], hiw^ΔN [60 (link)], hiw^ND8 [59 (link)], and UAS-bsk^DN were obtained from the Bloomington Drosophila Stock Center.

Singh P, & Donlea J.M. (2020). Bidirectional regulation of sleep and synapse pruning after neural injury. Current biology : CB, 30(6), 1063-1076.e3.

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Drosophila Neurogenic Amnesiac Mutant Analysis

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All the fly stocks were raised on standard cornmeal food at 25°C and 70% relative humidity on a 12:12 h light: dark cycle. The “Cantonized” w¹¹¹⁸ w(CS10) was used as the wild-type control. The C316-GAL4 (Bloomington stock number: 30830), amn¹ (Bloomington stock number: 5954), amn^28A, amn^C651, amn^X8, and UAS-amn⁺ flies have been described previously [6 (link),7 (link),21 (link),24 (link)]. VT64246-GAL4 and VT30604-GAL4 flies have been described previously [25 (link),30 (link)]. VT6412-GAL4 and VT49246-GAL4 flies were obtained from the Vienna Drosophila Resource Center (VDRC), Vienna Tile. The R16A06-GAL4 (Bloomington stock number: 48709) flies were obtained from Bloomington stock center. The tubP-GAL80^ts (Bloomington stock number: 7019); UAS-Kir2.1, and UAS-mCD8::GFP (Bloomington stock number: 5137); UAS-mCD8::GFP (Bloomington stock number: 5130) flies were gifts from Dr. Ann-Shyn Chiang.

Wu C.L., Fu T.F., Chou Y.Y, & Yeh S.R. (2015). A Single Pair of Neurons Modulates Egg-Laying Decisions in Drosophila. PLoS ONE, 10(3), e0121335.

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Drosophila Neurogenetics Experimental Setup

Cited 1 time

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Flies were maintained at 25 °C and raised on the standard cornmeal food under a 12:12-h light/dark cycle. The driver lines, GMR-Gal4 and GAD-Gal4, have been described previously^{40 (link),41 (link)}. The 202508-Gal4 line was obtained from the Vienna Drosophila Resource Center. UAS-mCD8-GFP, UAS-lacZ, UAS-Denmark, UAS-syt-GFP, tubP-Gal80^ts and UAS-shibire^ts were obtained from the Bloomington Drosophila Stock Center.

Chi H., Sun L., Shiu R.H., Han R., Hsieh C.P., Wei T.M., Lo C.C., Chang H.Y, & Sang T.K. (2020). Cleavage of human tau at Asp421 inhibits hyperphosphorylated tau induced pathology in a Drosophila model. Scientific Reports, 10, 13482.

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Drosophila Genetic Manipulation Protocol

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Ex-lacZ (#44248), nub-GAL4 (#25754), tubP-GAL80^ts (#7017) and scrib² (#41775) were obtained from the Bloomington Drosophila Stock Center (BDSC). Scrib:GFP (CA07683) was obtained from Fly Trap projects (Morin et al., 2001 (link)). αCat:GFP (#115921) was obtained from Kyoto Stock Center. Fly stocks were maintained at 25°C unless otherwise mentioned. To induce MARCM clones, larvae were heat-shocked for 2 h at 37°C 3 days after egg laying, and samples were collected 2 days after heat shock. Larvae and adults for conditional knockdown mediated by nub-GAL4 were maintained at 27°C after egg laying.

Huang Y., Gui J., Myllymäki S.M., Roy K., Tõnissoo T., Mikkola M.L, & Shimmi O. (2022). Scribble and α-Catenin cooperatively regulate epithelial homeostasis and growth. Frontiers in Cell and Developmental Biology, 10, 912001.

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Drosophila Genetic Stocks for Research

Cited 1 time

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Fly stocks were cultured on standard food medium at 25°C. Tet^AXXC, Tet^YRA, and USA-Gs2 fly strains were generated in this study. The following stocks were obtained from Bloomington Drosophila Stock Center (BDSC): nos-Cas9 (#54591), 3xP3-ECFP, alpha-tub-piggyBacK10/TM6C, Sb1 (#32072), UAS-mCD8:GFP, 201Y-GAL4 driver (#64296),²⁸ (link) enhancer trap Tet-T2A-GAL4-pA (#76666),³⁹ (link) multicolor flip-out stock MCFO-2 (#64086),³⁷ (link) Act5C-Cas9 (#54590), Gs2 TKO for Gs2 CRISPR/Cas9 mutagenesis (#76492), Gs2 RNAi (#92838),⁸⁰ (link) Gat RNAi (#29422), mCherry RNAi (#35785), tubP-GAL80^ts (#7017), and UAS-Dcr2 (#24651). Tet and Gat RNAi lines were obtained from Vienna Drosophila Resource Center (VDRC, GD#14330, KK#108964, and KK#107895). y¹ w^67c23; PCaryPattP40 was obtained from Dr. Norbert Perrimon.⁷² (link) Dilp2-GAL4 was originally obtained from Dr. Eric Rulifson (University of California, San Francisco).⁵⁹ (link) Fmr1³ (link) mutant was kindly provided by Dr. Thomas A. Jongens (University of Pennsylvania).²⁵ (link) All the Drosophila stocks in this paper are listed in the key resources table. This study was conducted in the mixed-sex Drosophila population with 50% males and females. The age and developmental stage for each experiment are provided in the method details.

Tran H., Le L., Singh B.N., Kramer J, & Steward R. (2024). Tet controls axon guidance in early brain development through glutamatergic signaling. iScience, 27(5), 109634.

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Genetic Tools for Drosophila Neural Development

Cited 3 times

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Fly strains used in this study include Oregon R, Ase-Gal4 (Zhu et al., 2006 (link)), Ase-Gal80 (Neumüller et al., 2011 (link)), brat^DG19310, brat^k06028 and brat¹¹ (Komori et al., 2014 (link)), erm¹ and erm² (Weng et al., 2010 (link)), erm-flag (Janssens et al., 2014 (link)), erm-lacZ and UAS-aPKC^CAAX (Haenfler et al., 2012 (link)), pnt^Δ88 (Morimoto et al., 1996 (link)), trx^Z11 (Tie et al., 2014 (link)), and Wor-Gal4 (Lee et al., 2006 (link)). The following stocks were obtained from the Bloomington Drosophila Stock Center: Elav-GAL4, Act-FRT-Stop-FRT-GAL4, ash2¹, btd^XA, FRT19A, FRT2A, FRT82B, GMR85C07-GAL4 (Btd-GAL4), hs-flp, P(EP)G4226, pros¹⁷, UAS-pnt_RNAi (TRiP.JF02227), UAS-pnt_RNAi (TRiP.HMS01452), trx^E2, tubP-Gal80, tubP-Gal80^ts, UAS-Dcr-2.D, UAS-mCD8-GFP, and UAS-trr_RNAi (TRiP.JF03242). We obtained the following stocks from the Vienna Drosophila RNAi Center UAS-ash2_RNAi (100718), UAS-dSet1_RNAi (40683), UAS-pnt_RNAi (7171), UAS-rbbp5_RNAi (106139), UAS-trx_RNAi (108122), and UAS-wds_RNAi (105371). UAS-HA-btd, UAS-HA-pntP1, UAS-rbbp5^FL-myc, and UAS-rbbp5^SG-myc were generated in this study by cloning the cDNA cloned into p{UAST}attB vector. The transgenic fly lines were generated via ϕC31 integrase-mediated transgenesis (Bischof and Basler, 2008 (link)). The rbbp5 null allele was generated by imprecisely excising the P(EP)G4226 element.

Komori H., Xiao Q., Janssens D.H., Dou Y, & Lee C.Y. (2014). Trithorax maintains the functional heterogeneity of neural stem cells through the transcription factor Buttonhead. eLife, 3, e03502.

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Diverse Drosophila Transgenic Lines

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The following lines were obtained from the Bloomington Drosophila Stock Center: nSyb-GAL4 (#51635), vGlut-GAL4 (#26160), ChAT-GAL4 (#6793), vGAT-GAL4 (#58409), Trh-GAL4 (#38389), Tdc2-GAL4 (#9313), OK107-GAL4 (#854), Akh-GAL4 (#25683), Mip-GAL4 (#51983), Ilp2-GAL4 (#37516), Eth-GAL4 (#51982), Fmrfa-GAL4 (#56837), Lk-GAL4 (#51993), NPF-GAL4 (#25681), phm-GAL4 (#80577), ple-GAL4 (#8848), SifAR-GAL4 (#76670), Tk-GAL4 (#51973), Eip93F RNAi (#57868), EcR RNAi (#58286), vGAT RNAi (#41958), and tubP-GAL80ts (#7019). E93-RNAi, nSyb-GAL4, mCherry-RNAi lines were outcrossed for five generations to a Berlin background (originally from the Heisenberg lab). Flies were raised on standard cornmeal/molasses food and incubated at 25 °C under a 12 h light/12 h dark cycle.

Yip C., Wyler S.C., Liang K., Yamazaki S., Cobb T., Safdar M., Metai A., Merchant W., Wessells R., Rothenfluh A., Lee S., Elmquist J, & You Y.J. (2024). Neuronal E93 is required for adaptation to adult metabolism and behavior. Molecular Metabolism, 84, 101939.

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