Mice were treated with 15mg/kg Cimetidine or vehicle (control) for 10 days intraperitoneally. A 3 mm length of the spinal cord centered on the lesion site was collected for analysis. Total RNA was extracted using RNeasy plus universal kit (Cat. No. 73404) followed by reverse transcription with the RT2 first strand kit (Cat. No. 330401) followed by RT2 profiler cytokine array (PAMM-150Z) according to the manufacturer's instructions (Qiagen). We screened 84 inflammation associated genes, and the data obtained were analyzed with the online Qiagen analysis software - RT2 profiler PCR array data analysis V3.5.
Rt2 profiler cytokine array pamm 150z
Manufactured by Qiagen
The RT2 Profiler Cytokine Array (PAMM-150Z) is a qPCR-based array designed to simultaneously detect the expression of 84 key cytokine genes in a single reaction. The array provides a comprehensive analysis of cytokine gene expression levels in a given sample.
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Lab products found in correlation
2 protocols using rt2 profiler cytokine array pamm 150z
1
Cimetidine Modulates Spinal Cord Inflammation
2
Cytokine Profiling in Spinal Cord Injury
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After harvesting spinal cord tissue of female wild-type and CCL3−/− mice at days 7 and 21 after injury (n = 3 per group and time point), RNA was extracted and quantified as described above. This was followed by reverse transcription using the RT2 first strand kit and RT2 profiler cytokine array (PAMM-150Z) according to the manufacturer’s instructions (Qiagen). Array results were analyzed using the GeneGlobe software tools provided by the manufacturer. Only genes significantly up- or down- regulated at least 2-fold are shown.
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