Vision tests were performed using a virtual optomotor system (Cerebral Mechanics)68 (link). Mice were placed on an elevated platform in the center of an area surrounded by four monitors. A cylinder comprised with a sine wave grating was drawn in virtual three-dimensional space and rotated around the animal. During testing, the behavior of the animal was tracked by a camera mounted above. Lack of tracking the sine wave grating with reflexive head and neck movements was indicative for an inability to resolve the visual pattern. A randomized simple staircase test was used to quantify vision thresholds. Rotation speed and contrast were set to 12.0 d/s and 100%, respectively.
Virtual optomotor system
Manufactured by Cerebral Mechanics
Sourced in Canada
The Virtual Optomotor System is a laboratory equipment designed to assess the visual function of small animals, such as rodents. It creates a virtual environment where the animal's head-tracking responses to moving visual stimuli can be recorded and analyzed. The system allows for the evaluation of various visual parameters, including contrast sensitivity, spatial frequency, and motion perception, without the need for physical movement or restraint of the animal.
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Lab products found in correlation
3 protocols using virtual optomotor system
1
Quantitative Virtual Optomotor Assay
2
Optomotor Vision Test Protocol for Mice
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Vision tests were performed between 9 am and 4 pm using a virtual optomotor system (Cerebral Mechanics, Lethbridge, Canada) as described previously.25 (link) Briefly, a rotating cylinder covered with a vertical sine wave grating was calculated and drawn in virtual three-dimensional space on four computer monitors arranged in a square. Visually unimpaired mice tracked the grating with reflexive head and neck movements (head-tracking). Vision threshold of the tested mice was quantified by a simple staircase test. Rotation speed and contrast were set to 12.0 d/s and 100%, respectively. Since no significant threshold differences were observed between males and females (P > 0.05; t-test), data of both sexes were combined. Thresholds of wild-type, heterozygous, and homozygous ALI030 mice (C57BL/6J background, age: 10 weeks) were compared using a linear mixed-effect model for grouped data (R package).
3
Optomotor Reflex Assessment in Mice
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The optokinetic reflex was determined using a virtual optomotor system (Cerebral Mechanics, Lethbridge, Canada) (28) . The mice were acclimated to room conditions for 30 min prior to examination in the drum to reduce murine agitation. A threshold for visual acuity was determined manually expressed as spatial frequency (measure/ combine mode) to obtain a comprehensive, whole visual system performance quantity (29, 30) . For this purpose, measurements started with a base value of just below 0.2 cycles/degree. The threshold was increased or decreased in steps of decreasing value depending on murine movement to approximate the final spatial frequency.
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