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Anti parp1 antibody

Manufactured by Thermo Fisher Scientific
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The Anti PARP1 antibody is a laboratory reagent that binds to and detects the presence of the PARP1 protein. PARP1 is an enzyme involved in various cellular processes, including DNA repair and programmed cell death. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to identify and quantify PARP1 in biological samples.

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Anti γh2ax antibody, by Merck Group (2 mentions)

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PARP1

4 protocols using anti parp1 antibody

1

Immunohistochemical Analysis of PARP1 in Tissues

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Tissues were fixed in 4% paraformaldehyde/PBS, permeabilized and stained with anti PARP1 antibody (Invitrogen, PA5–16452).
Wilson T., Pirovano G., Xiao G., Samuels Z., Roberts S., Viray T., Guru N., Zanzonico P., Gollub M., Pillarsetty N., Reiner T, & Bargonetti J. (2021). PARP Targeted Auger Therapy in p53 Mutant Colon Cancer Xenograft Mouse Models. Molecular pharmaceutics, 18(9), 3418-3428.
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2

Chromatin Immunoprecipitation Assay

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Chromatin immunoprecipitation (ChIP) assay was performed as previously described (22 (link)). The following antibodies were used for the IP reactions: anti-KLF4 antibody (SC-20691, Santa Cruz) and anti-PARP1 antibody (46–0705, Invitrogen). DNA was analyzed by qPCR using the primers at the hTERT promoter, and the CCNA1 promoter was used as a negative control. All primer sequences for PCR are listed in Supplementary Table S3.
Hsieh M.H., Chen Y.T., Chen Y.T., Lee Y.H., Lu J., Chien C.L., Chen H.F., Ho H.N., Yu C.J., Wang Z.Q, & Teng S.C. (2017). PARP1 controls KLF4-mediated telomerase expression in stem cells and cancer cells. Nucleic Acids Research, 45(18), 10492-10503.
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3

Immunofluorescence Quantification of DNA Damage

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Cells were plated on a coverslip slide on the bottom of a 6-well plate and incubated overnight. 123I-MAPi, 127I-PARPi, or vehicle control were added to the media and cells were returned to the incubator overnight. Cells were then fixed in 4% paraformaldehyde/PBS, permeabilized and stained with anti γ-H2AX antibody (Millipore Sigma, 05–636) and anti PARP1 antibody (Invitrogen, PA5–16452). DAPI was used to localize nuclei. Coverslips were mounted on slides for microscopy imaging.
Pirovano G., Jannetti S.A., Carter L.M., Sadique A., Kossatz S., Guru N., De Souza Franca P.D., Maeda M., Zeglis B.M., Lewis J.S., Humm J.L, & Reiner T. (2020). Targeted brain tumor radiotherapy using an Auger emitter. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(12), 2871-2881.
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4

Quantifying DNA Damage Response

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Cells were plated on a coverslip slide on the bottom of a 6-well plate and incubated overnight. 123I-MAPi, 127I-PARPi, or vehicle control were added to the media and cells were returned to the incubator overnight. Cells were then fixed in 4% paraformaldehyde/PBS, permeabilized and stained with anti γ-H2AX antibody (Millipore Sigma, 05–636) and anti PARP1 antibody (Invitrogen, PA5–16452). DAPI was used to localize nuclei. Coverslips were mounted on slides for microscopy imaging. Microscopy was acquired using a confocal microscope at the MSK Molecular Cytology Core Facility.
Wilson T.C., Jannetti S.A., Guru N., Pillarsetty N., Reiner T, & Pirovano G. (2020). Improved radiosynthesis of 123I-MAPi, an Auger theranostic agent. International journal of radiation biology, 99(1), 70-76.
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