All the chemicals used were analytical-reagent grade, and the water was ultrapure, obtained with a Millipore Integral 3 water purification system. The chemicals included: hexane (Sigma-Aldrich, Germany), methanol (Sigma-Aldrich, Germany), boron fluoride (Sigma-Aldrich, Germany), sodium hydroxide (Sigma-Aldrich, Germany), N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) derivatization reagent (Sigma-Aldrich, Germany), acetonitrile RS (Carlo Erba, France), methanol for LC-MS (Honeywell, Germany), acetonitrile for HPLC (Sigma-Aldrich, Germany), ethanol (Sigma-Aldrich, Germany), sodium acetate (Sigma-Aldrich, Germany), aluminum chloride (Sigma-Aldrich, Germany), rutoside (rutin, Sigma-Aldrich, Germany), tannic acid (Sigma-Aldrich, Germany), hydrochloric acid 0.5 N (Sigma-Aldrich, Germany), Arnow reagent (sodium nitrite, Sigma-Aldrich, Germany), chlorogenic acid (Sigma-Aldrich, Germany), 2,2 diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, Germany), ascorbic acid (vitamin C) (Roth, Germany), 2,2′-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonic acid, diammonium salt (ABTS ammonium salt) (Sigma-Aldrich, Germany), potassium persulfate (Merck, Germany), trichloroacetic acid (Merck, Germany), ferric chloride (Sigma-Aldrich, Germany), phosphate buffer, and potassium hexacyanoferrate (III) solution pH 6.6 (Sigma-Aldrich, Germany).
Acetonitrile for hplc
Manufactured by Merck Group
Sourced in Germany, United States
Acetonitrile is a common solvent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. Acetonitrile is widely used as a mobile phase component in HPLC systems due to its favorable properties, including its ability to effectively separate and elute a variety of analytes.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (4 mentions)
Ethanol, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
L rhamnose, by Merck Group (2 mentions)
L arabinose, by Merck Group (2 mentions)
D glucose, by Merck Group (2 mentions)
Activated charcoal, by Merck Group (2 mentions)
Milli q synthesis a10, by Merck Group (2 mentions)
Methyl alcohol rpe, by Carlo Erba (2 mentions)
Ammonia solution 30 rpe, by Carlo Erba (2 mentions)
Glacial acetic acid rpe, by Carlo Erba (2 mentions)
Boron fluoride, by Merck Group (1 mentions)
Aluminum chloride, by Merck Group (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
Integral 3 water purification system, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
19 protocols using acetonitrile for hplc
1
Analytical Reagent Protocols for Phytochemical Analysis
2
Cryptotanshinone Extraction and Purification
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Cryptotanshinone of 99.26% purity (Selleckchem, Poznań, Poland), ≥99.9% methanol for HPLC (Chempur, Piekary Śląskie, Poland), 96% ethanol (Stanlab Sp. J., Lublin, Poland), ≥99.5% isopropanol (Fluka: BioChemika, Buchs, Switzerland), sodium hydroxide (Stanlab Sp. J.), 2-amino-2-methyl-1,3-propanediol (AMPD, Sigma-Aldrich, Poznań, Poland), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS, Sigma-Aldrich), 2,2′,2″-nitrilotriethanol (TEOA, Sigma-Aldrich), tris(2-propanol)amine (TIPA, Sigma-Aldrich), 96% sulfuric acid (VI) (Stanlab Sp. J., Lublin, Poland), 35–38% hydrochloric acid (HCl, POCH BASIC, Gliwice, Poland), acetonitrile for HPLC (Sigma-Aldrich), formic acid for HPLC (Sigma-Aldrich), and deionized water were used in the experimental procedures.
3
Stability Study of Kuvan® and Diterin®
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Kuvan®, batch number L141191, was purchased in an ordinary manner from a pharmacy with expiry date: 02/2020. The manufacture date was not available; however, the leaflet of Kuvan® indicated a 3-year shelf-life indicating a manufacture date of around February 2017. Diterin® was generously donated by Dipharma SA (Chiasso, Switzerland), batch No. 17107H2, manufacture date: 05/2017, and expiry date: 04/2020. Starting from T = 0, both products were stored at 40 °C and 75% Relative Humidity (RH) in a constant climate chamber model HPP110 (Memmert, Italy), V = 108 L. Samples of both products were -taken at T = 0 and 6 months for the purpose of analysis. Storage at 40 °C and 75% RH for 6 months are the conditions indicated by EMA for accelerated stability studies (Note for Guidance on Stability Testing, CPMP/ICH/2736/99).
Water HPLC Plus, acetonitrile for HPLC (≥99.9%), ammonium formate (reagent grade, 97%), formic acid (reagent grade, ≥95%), and sapropterin, were all purchased from Sigma Aldrich (Milan, Italy). Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O), phosphoric acid 85% (H3PO4), and ascorbic acid were purchased from Titolchimica S.p.A (Pontecchio Polesine, Italy). HPLC-UV analysis was carried out on a Shimadzu LC-10AD instrument equipped with detector, Shimadzu SPD-10A (Shimadzu Italia, Milan, Italy). A clarity software program was used for the chromatogram analysis.
Water HPLC Plus, acetonitrile for HPLC (≥99.9%), ammonium formate (reagent grade, 97%), formic acid (reagent grade, ≥95%), and sapropterin, were all purchased from Sigma Aldrich (Milan, Italy). Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O), phosphoric acid 85% (H3PO4), and ascorbic acid were purchased from Titolchimica S.p.A (Pontecchio Polesine, Italy). HPLC-UV analysis was carried out on a Shimadzu LC-10AD instrument equipped with detector, Shimadzu SPD-10A (Shimadzu Italia, Milan, Italy). A clarity software program was used for the chromatogram analysis.
4
Docetaxel-loaded Biodegradable Nanoparticles
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The materials used in this article are as follows: Docetaxel was purchased from Xieli Pharmaceutical Co, CHINA. Poly (ethylene glycol)methyl ether (mPEG, Mn = 2000), Stannous octoate ((Sn(Oct)2), SIGMA, USA), Acetonitrile for HPLC were purchased from Sigma, USA. Despunlactide (D,L-lactide) was obtained from Daigang Biomateril Co, CHINA. ε-Caprolactone (ε-CL) was purchased from AlfaAesar, USA. anhydrous alcohol (EtOH), sodium phosphate dibasic (NaH2PO4), Tween80 were purchased from Aladdin, CHINA. Sodium dihydrogen phosphate (NaH2PO4.2H2O) was purchased from KeLong Chemical Co, China. dimethylsulfoxide (DMSO) was purchased from Rgent Chemicals, CHINA. acetic acid was purchased from BODI chemical, CHINA.
5
Lipase-catalyzed Stereoselective Reactions
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In this study, we have employed different commercial immobilized lipase. Lipozyme®TL (TLL-IM), Lipozyme®435 (L435), Lipozyme®RM (RML-IM) and LipuraSelect (LS-IM) were kindly donated by Novozymes Spain (Madrid, Spain). Triacetin, (R)- and (S)-methyl mandelate, zinc chloride (ZnCl2), copper chloride (CuCl2), cobalt chloride (CoCl2), sodium chloride (NaCl) and acetonitrile for HPLC (gradient grade, ≥99.9%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical grade.
6
Authentic Ginsenoside Standards Characterization
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Authentic standards of
ginsenosides Rg1, Re, Rb1, Rd, 20(S)-Rg2, 20(R)-Rg2, 20(S)-Rh1, 20(R)-Rh1, Rd, Rg6, F4, Rk3, Rh4,
20(S)-Rg3, 20(R)-Rg3, Rk1, Rg5, CK, 20(S)-Rh2, 20(R)-Rh2, and Rd2 (C–O) and notoginsenosides R1, 20(S)-R2, 20(R)-R2, Fa, Fd(C-Mx1), Fe (C-Mc1), and T5 were purchased from the Sichuan Victory Biological Technology
Co., Ltd. (Sichuan, China). The solvents methanol and acetonitrile
for HPLC were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
The HSGF254 silica gel TLC plate was purchased from Yantai
Jiangyou Silicone Development Co., Ltd. (Shangdong, China). DEAE-cellulose
DE52 was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai,
China). Other analytical-grade reagents were purchased from commercial
sources.
ginsenosides Rg1, Re, Rb1, Rd, 20(S)-Rg2, 20(R)-Rg2, 20(S)-Rh1, 20(R)-Rh1, Rd, Rg6, F4, Rk3, Rh4,
20(S)-Rg3, 20(R)-Rg3, Rk1, Rg5, CK, 20(S)-Rh2, 20(R)-Rh2, and Rd2 (C–O) and notoginsenosides R1, 20(S)-R2, 20(R)-R2, Fa, Fd(C-Mx1), Fe (C-Mc1), and T5 were purchased from the Sichuan Victory Biological Technology
Co., Ltd. (Sichuan, China). The solvents methanol and acetonitrile
for HPLC were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
The HSGF254 silica gel TLC plate was purchased from Yantai
Jiangyou Silicone Development Co., Ltd. (Shangdong, China). DEAE-cellulose
DE52 was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai,
China). Other analytical-grade reagents were purchased from commercial
sources.
7
Magnolol and Chavicol Biosynthesis
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Magnolol, chavicol, and other reagents were procured from Solarbio. Methanol and acetonitrile for HPLC were acquired from Sigma-Aldrich (St. Louis, MO, USA). The host bacteria Escherichia coli originated from Miaoling (Wuhan, China).
8
Analytical Method for Sulfonamide Antibiotics
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Ammonium molybdate tetrahydrate (H24Mo7N6O24·4H2O) (99%), thioacetamide (TAA) (98%), sulfathiazole (STZ), sulfadimidine (SDD), sulfadiazine (SDZ), sulfamethoxazole (SMZ), sulfacetamide (ST), sulfachloropyridazine (SCD), N4-phthalylsulfathiazole (PST), succinylsulfathiazole (SST), sodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), methanol for HPLC (99.9%) and acetonitrile for HPLC (99.9%) were purchased from Sigma-Aldrich (St Louis, USA). Acetone, sodium hydroxide (NaOH) and hydrochloric acid (HCl) were purchased from Beijing Chemical Works (Beijing, China). Polyethylene glycol (PEG4000) was purchased from Merck (Germany). Ultrapure water (18.2 MΩ cm) was prepared using a Milli-Q Gradient ultrapure water system (Millipore, Milford, MA, USA).
The eight SAs were dissolved in a 0.1 mol l−1 NaOH solution to prepare standard stock solutions of 2.0 mg ml−1 for each SA, and these standard solutions were stored at 4°C and protected from light. The working solutions to be analysed were diluted with ultrapure water. The calibration standards of SAs were prepared with five levels of concentration in the range of 0.3 to 30 µg ml−1.
The eight SAs were dissolved in a 0.1 mol l−1 NaOH solution to prepare standard stock solutions of 2.0 mg ml−1 for each SA, and these standard solutions were stored at 4°C and protected from light. The working solutions to be analysed were diluted with ultrapure water. The calibration standards of SAs were prepared with five levels of concentration in the range of 0.3 to 30 µg ml−1.
9
Quantitative Analysis of Bioactive Compounds
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Naringenin, apigenin, eriodictyol, pinocembrin, liquiritigenin, and other standards were bought from Yuanye Bio-Technology (Shanghai, China). 2-OH pinocembrin was bought from Chengdu SinoStandards Bio-Tech (Chengdu, China). Methanol and acetonitrile for HPLC were bought from Sigma-Aldrich (St. Louis, MO, USA). MeSA solution used for treatment was bought from Sangon Biotech (Shanghai, China).
10
Synthesis and Characterization of Polymer Composites
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LEVO sodium pentahydrate ≥99% was obtained from Enke Pharma-tech Co., Ltd, (Cangzhou, China). Trifluoroacetic acid (TFA) ≥99%, acetonitrile for HPLC, ethanol ≥99.8% for HPLC, bovine serum albumin (BSA) lyophilised powder ≥ 96%, poly(ethylene glycol (PEG) (M w 1,000) were obtained from Sigma Aldrich (Dorset, UK). Sodium azide (SA) was purchased from Fluorochem Ltd (Hadfield, UK). Poly(caprolactone) (PCL) 6506 (M W = 50,000 Da), henceforth referred to as H-PCL, and PCL 2054 (M W =550 Da), henceforth referred to as L-PCL were kindly donated by Perstorp (Malmö, Sweden). Poly(lactic acid) (PLA) filament was obtained from Ultimaker B.V. (Geldermalsen, Netherlands). Dichloromethane (DCM) was obtained from Merck (Darmstadt, Germany). Finally, Silastic ® S, a silicone rubber and curing agent mix were obtained from Thompson Bros. Ltd. (Newcastle Upon Tyne, UK).
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