Anti human foxp3 staining set 236a e7

Manufactured by Thermo Fisher Scientific

The Anti-Human FoxP3 Staining Set (236A/E7) is a lab equipment product designed for the detection and quantification of human FoxP3-positive regulatory T cells (Tregs) using flow cytometry. The set includes a fluorochrome-conjugated antibody that binds to the FoxP3 transcription factor, which is a key marker for Tregs.

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Lab products found in correlation

Streptavidin apc cy7, by BD (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Human fc receptor binding inhibitor, by Thermo Fisher Scientific (1 mentions) Anti cd56 bv421 hcd56, by BioLegend (1 mentions) Anti cd8a apc cy7 hit8a, by BioLegend (1 mentions) Cd25 pe, by BioLegend (1 mentions) Cd59 pe, by BioLegend (1 mentions) Cd4 pe rpa t4, by BD (1 mentions) Saponin, by Merck Group (1 mentions) Facscanto 2, by BD (1 mentions)

Close spelling variants of this product

Anti-Foxp3 (119 citations) Foxp3 Staining Set (28 citations) Foxp3 (236A/E7; (12 citations) Anti-Human Foxp3 Staining Set (12 citations) Anti-Human Foxp3 Staining Set APC (clone 236A/E7) (2 citations)

2 protocols using anti human foxp3 staining set 236a e7

Multi-Marker Flow Cytometry Analysis

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The following antibodies and reagents were used for sample analysis by flow cytometry: Human Fc receptor binding inhibitor (eBioscience), Anti-CD45 PE-Cy7 (HI30; eBioscience), anti-CD11b PE (M1/70; BD Biosciences), anti-CD56 BV421 (HCD56; Biolegend), anti-CD3 PerCp-Cy5.5 (OKT3, eBioscience), anti-CD4 Alexa Fluor 700 (OKT4; eBioscience), anti-CD8a APC-Cy7 (HIT8a; Biolegend), anti-Vα24 PE (C15; Beckman Coulter), anti-Vβ11 FITC (C21; Beckman Coulter), anti-BDCA-2 APC (201A; Biolegend), anti-TCRγδ PE-CF594 (B1; BD Biosciences), anti-CD11c BV421 (3.9; Biolegend), anti-HLA-DR PE-CF594 (G46-6; BD Biosciences), anti-Cytokeratin 10 biotin (DE-K10; Abcam), anti-Cytokeratin 14 FITC (LL002; Abcam), Streptavidin-APC-Cy7 (BD Biosciences), and the anti-Human FoxP3 Staining Set (236A/E7; eBioscience). Rat IgG2a,κ Isotype Control APC (R35-95; BD Biosciences) was used as an isotype control for intracellular FoxP3 staining. Dead cells were excluded using the LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies, New York, U.S.) according to the manufacturers’ instructions, and anti-CD45 was used to differential immune cells from non-immune cells.

Freeman A., Bridge J.A., Maruthayanar P., Overgaard N.H., Jung J.W., Simpson F., Prow T.W., Soyer H.P., Frazer I.H., Freeman M, & Wells J.W. (2014). Comparative Immune Phenotypic Analysis of Cutaneous Squamous Cell Carcinoma and Intraepidermal Carcinoma in Immune-Competent Individuals: Proportional Representation of CD8+ T-Cells but Not FoxP3+ Regulatory T-Cells Is Associated with Disease Stage. PLoS ONE, 9(10), e110928.

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Comprehensive Immunophenotyping of Endothelial Cells

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Flow cytometry was carried out on a FACS Canto II (BD Biosciences). Endothelial cells were phenotyped with the following antibodies: HLA-DR APC (L243), HLA-DQ PE (HLA-DQ1), HLA-ABC APC/Cy7 (W6/32), VE Cadherin PerCP/Cy5.5 (BV9), PD-L1 PE/Cy7 (29E.2A3), CD59 PE [P282 (H19)], CD55 FITC (JS11) and CD46 PE/Cy7 (TRA-2-10; Biolegend) and ICAM-1 FITC (84H10; Beckman Coulter). Lymphocyte polarization was analyzed using: CD4 PE (RPA-T4; BD Pharmingen), IFN-γ FITC (B27; BD Biosciences), CD3 PerCP (SK7; Becton Dickinson); CD4 PB (RPA-T4), CD8 PB (RPA-T8), CD45RA PE/Cy7 (H100), CD25 PE (M-A251), CD127 PerCP/Cy5.5 (A019D5), CD185 APC/Cy7 (J252D4; Biolegend) and IL-17 efluor660 (eBioscience). FoxP3 was detected using the anti-Human Foxp3 Staining Set (236A/E7; eBioscience). Intracellular staining of HLA-DR and HLA-DQ was carried out on fixed and permeabilised cells (2% PFA (Sigma) and 0.5% saponin (Sigma)). Intracellular staining of phosphorylated Akt was carried out in fixed and permeabilised cells (1.5% PFA followed by pure methanol (Millipore) at -20°C) using the following antibodies pAkt (Ser473) APC (SDRNR) and anti-mouse IgG Alexa Fluor^® 647 (Poly4053; Biolegend).

Cross A.R., Lion J., Poussin K., Glotz D, & Mooney N. (2021). Inflammation Determines the Capacity of Allogenic Endothelial Cells to Regulate Human Treg Expansion. Frontiers in Immunology, 12, 666531.

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