IPSC-CMs were dissociated with 0.25% trypsin-EDTA and then fixed with CytoFix fixation buffer (BD) for 20 min. The cells were permeabilized with Perm/Wash buffer (BD) at room temperature for 10 min and then incubated with mouse anti-troponin T antibody (Thermo) for 30 min. Cells were washed with Perm/Wash buffer prior to incubation with the Alexa Fluor 488 rabbit anti-mouse IgG secondary antibody (Invitrogen) at room temperature for 30 min. These cells were analyzed on a FACS Canto II (BD).
Mouse anti troponin t antibody
Manufactured by Thermo Fisher Scientific
The Mouse anti-troponin T antibody is a laboratory reagent used to detect and quantify the presence of troponin T, a protein found in cardiac muscle cells. It can be used in various immunoassay techniques to measure troponin T levels in biological samples.
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Lab products found in correlation
2 protocols using mouse anti troponin t antibody
1
Immunophenotyping of Induced Pluripotent Stem Cell-Derived Cardiomyocytes
2
Cardiomyogenic Differentiation of miPSCs
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miPSCs that were cardiomyogenically differentiated were dissociated with 0.25% trypsin-EDTA, fixed with CytoFix fixation buffer (BD, Franklin Lakes, NJ) for 20 minutes, permeabilized with Perm/Wash buffer (BD) at room temperature for 10 min, and then incubated with mouse antitroponin T antibody (Thermo Scientific, Waltham, MA) for 30 min. The labeled cells were washed with Perm/Wash buffer prior to incubation with the Alexa Fluor 647 rabbit anti-mouse IgG secondary antibody (Invitrogen) at room temperature for 30 min, and then assayed by using a FACS Canto II (BD) followed by Flowjo software (Tree Star, Ashland, OR) analysis.
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