Gotaqpcr kits

Total RNA was extracted using the TRI Reagent^®isolation system (Sigma–Aldrich, St Louis, MO, United States). Moloney murine leukemia virus (M-MLV) reverse transcriptase and GoTaqPCR kits (Promega) were used for semiquantitative RT-PCR using primer sets as follows: HEY1, forward 5′-ATACGCCTGCATTTACCAGC-3′ and reverse 5′-TCAATTGACCACTCGCACAC-3′. Primer sets for NOTCH1, NOTCH2, and ACTB were published elsewhere (Hubmann et al., 2013 (link)). Real-time quantitative RT-PCR (qPCR) for NOTCH2 was performed with TaqMan^®-probes (Hs01050717_m1) purchased from Applied Biosystems (Thermo Fisher Scientific, Waltham, MA, United States).

Total RNA was extracted using the TRI Reagent^® isolation system (Sigma-Aldrich, St Louis, MO, USA). M-MLV reverse transcriptase and GoTaq PCR kits (Promega, WI, USA) were used for semi quantitative RT-PCR. The MYC primer sequences used in this study read as follows: forward 5’-GAAAACAATGAAAAGGCCCC-3’ and reverse 5’-TTCCTTACGCACAAGAGTTC-3’. Primer sets for NOTCH1, NOTCH2, NOTCH3, FCER2, NR4A1, and ACTB were published elsewhere [32 (link)]. PCR bands were stained with GelRedTM (Biotium, Fremont, CA, USA) and visualized using the ChemiDocTM gel imaging system from Bio-Rad (Hercules, CA, USA).