Albumin, prealbumin, and C-reactive protein (CRP) levels were measured with an ADVIA 2400 analyser (Siemens Healthcare, Spain). Folic acid, vitamin B12 and ferritin levels were measured with an ADVIA Centaur (Siemens Healthcare, Spain). sTfR levels were measured in a BN-II nephelometer (Dade-Siemens Healthcare, Spain) and vitamin A levels by reversed phase high-performance liquid chromatography [17 ]. Erythropoietin quantification was performed using the Quantikine human erythropoietin immunoassay kit (R&D Systems, USA).
Advia 2400 analyser
Manufactured by Siemens
Sourced in United Kingdom
The ADVIA 2400 analyser is a clinical chemistry and immunoassay system designed for high-volume clinical laboratories. It provides automated processing of a wide range of clinical chemistry and immunoassay tests. The ADVIA 2400 is capable of performing various analytical functions, including sample handling, reagent preparation, and result reporting.
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Lab products found in correlation
5 protocols using advia 2400 analyser
1
Biomarker Quantification in Clinical Research
2
Aortic Aneurysm Measurement in Mice
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BP was measured by a computer controlled non-invasive tail-cuff BP system (Kent Scientific Corporation, USA) on conscious mice (after one week of training) at 10 a.m., and recorded by the CODA program [26 (link)]. The levels of fasting serum liver alanine aminotransferase were measured using standard method of the ADVIA 2400 analyser (Siemens, UK). Food and water consumptions were measured daily and body weights were measured weekly as described previously [26 (link)]. Aortic aneurysm was defined as an increase of 50% or more in the maximum diameter of suprarenal aorta compared to saline-infused control mice as described previously [27 (link)]. The suprarenal aortic diameter and wall thickness were measured using ImageJ (National Institutes of Health, Bethesda, MD).
3
Plasma Electrolyte and Hormone Analysis
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At sacrifice blood samples were drawn from the abdominal aorta and, plasma samples were prepared by centrifugation at 3,000 rpm for 15 min. All samples were stored at – 20°C until analysis.
Aldosterone and renin activity were measured using radioimmunoassay [13 (link)]. Electrolytes (Na+, K+, Cl−, Mg2+, Ca2+) were measured using ion-selective electrode technique by ADVIA 2400 analyser (Siemens, Inc. Munich, Germany), and osmolality was measured using a vapor pressure osmometer 5600 (Wescor, Inc. Logan, UT, USA). Urinary electrolytes and aldosterone were corrected for creatinine.
Aldosterone and renin activity were measured using radioimmunoassay [13 (link)]. Electrolytes (Na+, K+, Cl−, Mg2+, Ca2+) were measured using ion-selective electrode technique by ADVIA 2400 analyser (Siemens, Inc. Munich, Germany), and osmolality was measured using a vapor pressure osmometer 5600 (Wescor, Inc. Logan, UT, USA). Urinary electrolytes and aldosterone were corrected for creatinine.
4
HbA1c and Urine Creatinine Measurement
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HbA1c was measured by boronate affinity chromatography using a Primus Ultra2 HPLC system (Primus Diagnostics, Kansas City, USA). Urine creatinine measurement was performed using a kinetic Jaffé reaction on an Advia 2400 analyser (Siemens Healthcare Diagnostics, Frimley, UK).
5
Measurement of Glycated Hemoglobin and Albumin
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Glycated haemoglobin (HbA1c) was determined by liquid chromatography on an Adams A1C HA-8160 analyser (Arkray, Kyoto, Japan); HbA1c values were converted to units aligned with the Diabetes Control and Complications Trial, according to the US National Glycohemoglobin Standardization Program [47 (link)].
Fructosamine levels were determined by the diazyme glycated serum protein enzymatic method (Diazyme, Kent, UK) on an Advia 2400 analyser (Siemens). This assay uses protein K to digest serum proteins into small fragments and fructosaminase to catalyse the specific oxidation of the ketoamine linkage of glycated fragments. The release of hydrogen peroxide, measured colorimetrically at 550 nm, is proportional to the concentration of glycated serum proteins (fructosamine). To calculate the percentage of glycated albumin, the values of fructosamine (glycated serum proteins) were determined using the aforementioned method and those of albumin using the bromocresol green method. The glycated albumin percentage was calculated following the equation recommended by the manufacturer [48 (link)].
Fructosamine levels were determined by the diazyme glycated serum protein enzymatic method (Diazyme, Kent, UK) on an Advia 2400 analyser (Siemens). This assay uses protein K to digest serum proteins into small fragments and fructosaminase to catalyse the specific oxidation of the ketoamine linkage of glycated fragments. The release of hydrogen peroxide, measured colorimetrically at 550 nm, is proportional to the concentration of glycated serum proteins (fructosamine). To calculate the percentage of glycated albumin, the values of fructosamine (glycated serum proteins) were determined using the aforementioned method and those of albumin using the bromocresol green method. The glycated albumin percentage was calculated following the equation recommended by the manufacturer [48 (link)].
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