Mouse anti becn1

Manufactured by Cell Signaling Technology

Mouse anti-BECN1 is a primary antibody that recognizes the BECN1 protein. BECN1 is a component of the autophagy initiation complex and plays a crucial role in the regulation of autophagy.

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Lab products found in correlation

2 protocols using mouse anti becn1

Autophagy Regulation via Calcium Oxalate

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Calcium oxalate (Sigma, 455997), 3-methyladenine (Sigma, M9281), rapamycin (Sigma, R0395), 4′,6-diamidino-2-phenylindole (Sigma, D8417), N-acetyl-L-cysteine (Sigma, A7250), catalase (Millipore, 219261-100KU), 2′,7′-dichlorofluorescin diacetate (Sigma, D6883), Lipofectamine 3000 (Invitrogen, L3000008), and rabbit anti-LC3B (Sigma, L7543) for western blot (WB) (1:2000), mouse anti-BECN1 (Cell Signaling Technology, 3495) for WB (1:1000). rabbit anti-LC3B (Abcam, ab51520) (1:1000) and mouse anti-BECN1 (Abcam, ab114071) were used for immunohistochemistry (IHC) (1:500). Mouse monoclonal anti-GAPDH (Proteintech, 60004-1-Ig) was used for WB (1:5000). Mouse and rabbit HRP-conjugated antibodies were obtained from Zhongshan Golden Bridge Biotechnology (ZB-2305, ZB- 2301).

Liu Y., Li D., He Z., Liu Q., Wu J., Guan X., Tao Z, & Deng Y. (2017). Inhibition of autophagy-attenuated calcium oxalate crystal-induced renal tubular epithelial cell injury in vivo and in vitro. Oncotarget, 9(4), 4571-4582.

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Molecular Tools for Autophagy Research

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HA-NEDD4^WT (Addgene, 27002), and HA-NEDD4^C867A (Addgene, 26999) were gifts from Dr. Joan Massague.⁵⁸ (link) MYC-ULK1^WT (Addgene, 27629), MYC-ULK1^K46I (Addgene, 27630), and MYC-ULK1^S4A (Addgene, 27631) were gifts from Dr. Reuben Shaw.⁵⁹ (link) FLAG-ubiquitin WT and related mutants (K6WT, K11WT K27WT, K29WT, K33WT, K48WT, K63WT and KallR) were provided by the MRC Protein Phosphorylation and Ubiquitylation Unit, UK. YFP-Atg8-family proteins encoding plasmids were a kind gift from Dr. Felix Randow (MRC Laboratory of Molecular Biology, UK). MYC-BECN1 plasmid (Biocat GmbH, MR207162-OR), MG132 (Merck Chemicals GmbH, 474791), Torin1 (Tocris Bioscience, 4247), cyclohexamide (Sigma-Aldrich, C1988) and choloroquine (Sigma-Aldrich, C6628) were purchased as indicated. Antibodies used in this study include: anti-ACTB (Sigma-Aldrich, A2228), anti-LC3 (MBL International, PM036), anti-human NEDD4 (Cell Signaling Technology, 3607), anti-mouse NEDD4 (BD Biosciences, 611481), anti-K48 ubiquitin (Merck Millipore, 05–1037), anti-K63 ubiquitin (Enzo Life Sciences, HWA4C4), anti-ubiquitin (FK2) (Enzo Life Sciences, BML-PW8810), rabbit anti-BECN1 (Cell Signaling Technology, 3495), mouse anti-BECN1 (Cell Signaling Technology, 4122), anti-ULK1 (Cell Signaling Technology, 8054), anti-LC3B (Sigma-Aldrich, L7543), anti-LAMP2 (Santa Cruz Biotechnology, SC-18822).

Pei G., Buijze H., Liu H., Moura-Alves P., Goosmann C., Brinkmann V., Kawabe H., Dorhoi A, & Kaufmann S.H. (2017). The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy. Autophagy, 13(12), 2041-2055.

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