The head and neck squamous cell carcinoma cell lines OSC19, UMSCC1, Tu138, FADU, UMSCC-22A cells were maintained as described previously [28 (link), 29 (link)]. The OSC19-Luc cell line is a stable transfectant constitutively expressing luciferase and GFP [30 (link)]. Normal human fibroblasts (Detroit 551) were purchased from ATCC. Recombinant human BDNF and EGF were obtained from Peprotech (Rocky Hill, NJ). The following antibodies were utilized: TrkA (sc-14024, Santa Cruz Biotechnology, Inc.), TrkB (sc-8316), TrkC (sc-117), NGF (sc-548), BDNF (sc-546), vimentin (sc-51719), GAPDH, MAPK (9107, Cell Signaling, Inc.), phospho-MAPK (9101), STAT3 (9132), phospho-STAT3 (9145), AKT (9272), phospho-AKTser473 (3787), Twist (4119), E-cadherin (4065), N-cadherin (4061), β-catenin (9562), PCNA (2586), Slug, Snail and LYVE-1.
Normal human fibroblasts detroit 551
Manufactured by American Type Culture Collection
Normal human fibroblasts (Detroit 551) are a cell line derived from the skin of a healthy adult human. They are a widely used model system for the study of cellular processes and functions.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human bdnf, by Thermo Fisher Scientific (2 mentions)
Phospho stat3 9145, by Cell Signaling Technology (2 mentions)
Twist, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
E cadherin, by Cell Signaling Technology (2 mentions)
β catenin, by Cell Signaling Technology (2 mentions)
Phospho akt ser473, by Cell Signaling Technology (2 mentions)
Trka sc 14024, by Santa Cruz Biotechnology (2 mentions)
N cadherin, by Cell Signaling Technology (2 mentions)
2 protocols using normal human fibroblasts detroit 551
1
Characterization of Head and Neck Cancer Cell Lines
2
Characterization of Head and Neck Cancer Cell Lines
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The head and neck squamous cell carcinoma cell lines OSC19, UMSCC1, Tu138, FADU, UMSCC-22A cells were maintained as described previously [28 (link), 29 (link)]. The OSC19-Luc cell line is a stable transfectant constitutively expressing luciferase and GFP [30 (link)]. Normal human fibroblasts (Detroit 551) were purchased from ATCC. Recombinant human BDNF and EGF were obtained from Peprotech (Rocky Hill, NJ). The following antibodies were utilized: TrkA (sc-14024, Santa Cruz Biotechnology, Inc.), TrkB (sc-8316), TrkC (sc-117), NGF (sc-548), BDNF (sc-546), vimentin (sc-51719), GAPDH, MAPK (9107, Cell Signaling, Inc.), phospho-MAPK (9101), STAT3 (9132), phospho-STAT3 (9145), AKT (9272), phospho-AKTser473 (3787), Twist (4119), E-cadherin (4065), N-cadherin (4061), β-catenin (9562), PCNA (2586), Slug, Snail and LYVE-1.
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