The human neuroblastoma cell line SH-SY5Y was obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). The cells were grown in a monolayer at 37 °C in 5% CO2 humidified atmosphere in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F12) medium (Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich). In order to induce differentiation, SH-SY5Y cells were exposed to 10 µM of RA (Sigma-Aldrich) for 5 days.
F 12 ham dmem f12 medium
Manufactured by Merck Group
Sourced in United States
F-12 Ham (DMEM/F12) medium is a cell culture medium formulated for the growth and maintenance of mammalian cells. It is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture, designed to provide the necessary nutrients and growth factors for cell proliferation and viability.
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3 protocols using f 12 ham dmem f12 medium
1
Neuroblastoma SH-SY5Y Cell Differentiation
2
Neuroblastoma Cell Differentiation with RA
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The human neuroblastoma cell line SH-SY5Y was acquired from American Type Culture Collection (ATCC) (Manassas, VA, USA). Cells were grown in a monolayer at 37 °C in a 5% CO2 humidified atmosphere using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F12) medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), 1% glutamine, and 1% penicillin-streptomycin (100 U-100 µg/mL). With the aim of inducing neuronal differentiation, SH-SY5Y cells were incubated for 5 days with 10 µM of RA (Sigma-Aldrich).
3
SH-SY5Y Cell Differentiation with Retinoic Acid
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The human neuroblastoma cell line SH-SY5Y was obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). The SH-SY5Y cells were cultured in monolayer using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F12) medium (Sigma-Aldrich, Saint Louis, MO, USA) containing 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich, Saint Louis, MO, USA), glutamine, and penicillin-streptomycin. Cells were grown at 37 °C in a moisturized atmosphere of 5% CO2 and 95% air. After culture, SH-SY5Y cells were exposed to 10 µM of retinoic acid (RA) for 5 days, to induce cellular differentiation.
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