Series of five micrometer‐thick sections from frozen liver biopsies and controls were cut and immunostained with primary antibodies using a peroxidase‐labeled polymer method (Envision Flex Plus kit, Dako) in a Dako autostainer. A light counterstaining was obtained with Meyer hematoxylin for 60 seconds. Numbers of CD3, CD4, CD8, forkhead box P3 (FoxP3), CD161, T cell receptor (TCR) Vα7.2, and granzyme B (GrB) positive cells were quantified in lobule areas, within the interface hepatitis and portal tract areas. Immunolabeled cells present in these areas were counted in three different fields at 400 × magnification (100,000 μm2 each) using the NE Elements Imaging Software (Nikon). Liver biopsies from organ donors after cardiac death (stage III of the Maastricht classification) served as normal controls. Immunofluorescence staining was performed on the same frozen liver biopsies using CD3, CD161, and TCR Vα7.2 antibodies, and analyzed by confocal microscopy.
Envision flex plus kit
Manufactured by Agilent Technologies
Sourced in Denmark
The Envision Flex Plus kit is a laboratory equipment product offered by Agilent Technologies. It is a versatile and flexible system designed for a range of analytical applications. The core function of the Envision Flex Plus kit is to provide a platform for assays and experiments that require precise measurement and analysis.
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Lab products found in correlation
2 protocols using envision flex plus kit
1
Immunohistochemical Analysis of Liver Immune Cells
2
Immunohistochemistry of Formalin-Fixed Tissue
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Three micrometer sections of selected formalin-fixed paraffin-embedded tissue blocks were immunostained with the primary antibodies detailed in Table 1 using peroxydase-labeled polymer method (Envision Flex Plus kit, Dako, Glostrup, Denmark) in a Dako Autostainer after elimination of endogenous peroxydase.
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