HLA-A2 transgenic mice [B6.Cg-Tg(HLA-A/H2-D)2Enge/J] purchased from the Jackson Laboratory (stock no: 004191; Bar Harbor, ME, USA) and nude mice purchased from the Nanjing Biomedical Research Institute (Nanjing University, Nanjing, China) were kept under standard pathogen-free conditions. Female mice (6–8 weeks old) were weight-matched for use in different experimental groups. All the animals were cared for according to the Animal Care Guidelines of the Third Military Medical University. The human lung cancer cell line, A549 (TCHu150), and HepG2 (TCHu72) liver cancer cells, were purchased from the cell library of the Chinese Academy of Sciences. A549-luciferase (A549-Luc) was purchased from Shanghai Model Organisms Center (NM-F04-1). The Plasmodium berghei (P. b.) ANKA strain was a gift from a senior investigator, Xinzhuan Su, of National Institute of Allergy and Infection Diseases (NIAID), Bethesda, MD, USA.
B6 cg tg hla a h2 d 2enge j
Manufactured by Jackson ImmunoResearch
Sourced in United States
B6.Cg-Tg(HLA-A/H2-D)2Enge/J is a transgenic mouse strain that expresses human leukocyte antigen (HLA) class I molecules. The core function of this strain is to serve as a model for studying immune responses and the expression of HLA molecules.
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4 protocols using b6 cg tg hla a h2 d 2enge j
1
Preparation of HLA-A2 Transgenic and Nude Mice for Cancer Research
2
Breeding Transgenic Mouse Strains
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BL/6, BL/6-Foxp3gfp × Thy1.1 BL/6, HLA-A2+ BL/6 mice [B6.Cg-Tg(HLA-A/H2-D)2Enge/J], and OTII BL/6 mice [B6.Cg-Tg(TcraTcrb)425Cbn/J] were purchased from Jackson Laboratory and bred in-house under specific pathogen–free conditions.
3
Evaluating HLA-A2 Peptide Vaccines
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HLA-A2 transgenic mice, B6.Cg-Tg (HLA-A/H2-D)2Enge/J (Jackson Laboratory, USA), maintained in house, or C57BL/6 mice (Charles River, Germany) were vaccinated with the indicated peptides at their respective doses in a volume of 100 μl. Peptides were adjuvanted with Incomplete Freund’s Adjuvant (IFA) (1/1 (V/V)) and CpG (50 μg/mouse) by vortexing the mixture for 30 minutes. In all experiments, mice were subcutaneously vaccinated at days 0 and 21 in alternating flanks. Two weeks after booster vaccination, mice were sacrificed, spleens were excised and spleen cells were restimulated for 16 hours with WT peptide or CPL. Specific IFN-γ responses were assessed using an ELISpot assay.
4
Breeding Transgenic Mouse Strains
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BL/6, BL/6-Foxp3gfp × Thy1.1 BL/6, HLA-A2+ BL/6 mice [B6.Cg-Tg(HLA-A/H2-D)2Enge/J], and OTII BL/6 mice [B6.Cg-Tg(TcraTcrb)425Cbn/J] were purchased from Jackson Laboratory and bred in-house under specific pathogen–free conditions.
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