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Ab201456

Manufactured by Abcam
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Sourced in United States, United Kingdom

Ab201456 is a laboratory equipment product. It is designed for general laboratory use. No further details about its core function or intended use can be provided in an unbiased and factual manner.

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Lab products found in correlation

Ab181602, by Abcam (2 mentions) Ab191433, by Abcam (1 mentions) Ab9050, by Abcam (1 mentions) Ab26350, by Cell Signaling Technology (1 mentions) Rabbit anti phospho smad2 ser465 467, by Cell Signaling Technology (1 mentions) Rabbit anti smad2, by Cell Signaling Technology (1 mentions) Ab52865, by Abcam (1 mentions) Hrp conjugated anti rabbit igg, by GE Healthcare (1 mentions) Ab76148, by Abcam (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Qproteome cell compartment kit, by Qiagen (1 mentions) Ecl plus, by GE Healthcare (1 mentions) Ab9485, by Abcam (1 mentions) Ab133741, by Abcam (1 mentions) Ab236256, by Abcam (1 mentions) Ab216324, by Abcam (1 mentions) Ab37415, by Abcam (1 mentions) Ab7291, by Abcam (1 mentions) Esl103, by Abcam (1 mentions) Ripa lysis buffer, by Merck Group (1 mentions)

6 protocols using ab201456

1

Immunodetection of Cellular Targets

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Primary antibodies used in this study were as follows: polyclonal anti-ɑ-tubulin (Sigma, catalog no.T6199), anti-RFXAP (Abcam, ab9258; ab87251), anti-KDM4A (Abcam, ab191433), anti-H3 (Abcam, ab201456), anti-H3K36me3 (Abcam, ab9050), anti-γ-H2AX(Abcam, ab26350; Cell Signaling Technology, catalog no. #9718S).
Ding G., Xu X., Li D., Chen Y., Wang W., Ping D., Jia S, & Cao L. (2020). Fisetin inhibits proliferation of pancreatic adenocarcinoma by inducing DNA damage via RFXAP/KDM4A-dependent histone H3K36 demethylation. Cell Death & Disease, 11(10), 893.
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2

Comprehensive Antibody Panel Analysis

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Primary antibodies used in the current study included the following: rabbit anti-PTEN, 9552; rabbit ant-phospho S6 (Ser235/236), 4858; S6, 2217, rabbit anti-phospho-4E-BP1 (Thr37/46), 2855; rabbit anti-phospho-Akt (Ser473), 4060; rabbit anti-phospho-Akt (Thr308), 4056; rabbit anti-Akt, 9272; rabbit anti-FoxO1, 2880; rabbit anti-phospho-FoxO1 (Ser256), 8419; rabbit anti-Smad2, 5339; rabbit anti-phospho-Smad2 (Ser465/467), 18338 (Cell Signaling Technology, Bever, USA); rabbit anti-NOX2, NB2-41291 and -NOX4, NB110-58849 (Novus Biologicals, USA); rabbit ant-CSE (MyBioSource, USA MBS 769567); rabbit anti-PAX7, ab92317; and the housekeeping genes rabbit GAPDH, ab181602, and histone H3, ab201456 (Abcam, USA).
Bitar M.S., Nader J., Al-Ali W., Al Madhoun A., Arefanian H, & Al-Mulla F. (2018). Hydrogen Sulfide Donor NaHS Improves Metabolism and Reduces Muscle Atrophy in Type 2 Diabetes: Implication for Understanding Sarcopenic Pathophysiology. Oxidative Medicine and Cellular Longevity, 2018, 6825452.
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3

Western Blot Analysis of MSI1, MSI2, and Tau

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Western blot analyses were performed with iHEK cell samples. Approximately 10 µg of protein preparations were loaded on precast NuPAGE 4%–12% Bis‐Tris gels (Invitrogen) for SDS‐PAGE analyses. Gels were subsequently transferred onto nitrocellulose membrane and blocked overnight at 4°C with 10% nonfat dry milk. Membranes were then probed for 1 hr at room temperature with α‐MSI1 (1:1,000, ab52865 Abcam), α‐MSI2 (1:1,000, ab76148 Abcam), Pan‐Tau (Tau13—1:10,000, MMS‐520R Covance), GAPDH (1:1,000, ab9485 Abcam), LaminB1 (1:1,000, ab133741 Abcam), and Histone3 (1:1,000, ab201456 Abcam) antibodies diluted in 5% nonfat dry milk. α‐MSI1 immunoreactivity and α‐MSI2 immunoreactivity were detected with an HRP‐conjugated anti‐rabbit IgG (1:6,000, GE Healthcare). Tau13 immunoreactivity and GAPDH immunoreactivity were detected using an anti‐mouse IgG (1:6,000, GE Healthcare) diluted in 5% milk. ECL plus (GE Healthcare) was used to visualize the bands. LaminB1 and GAPDH were used to normalize and quantify the amount of nuclear and cytoplasmic proteins, respectively. The compartment extraction was conducted with Qproteome Cell Compartment Kit (Qiagen, #37502), and nuclear, cell membrane, and cytoplasmic proteins were isolated and preserved for Western analysis.
Montalbano M., McAllen S., Sengupta U., Puangmalai N., Bhatt N., Ellsworth A, & Kayed R. (2019). Tau oligomers mediate aggregation of RNA‐binding proteins Musashi1 and Musashi2 inducing Lamin alteration. Aging Cell, 18(6), e13035.
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4

Comprehensive Protein Detection Protocol

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Protein extracts were prepared with RIPA Lysis buffer (Millipore) containing 50 mM Tris–HCl, pH 7.4, 1% Nonidet P-40, 0.25% sodium deoxycholate, 500 mM NaCl, 1 mM EDTA, and 1 × protease inhibitor cocktail (Roche Applied Science). To detect LGALS3BP secreted protein, cell supernatant with trichloroacetic acid precipitation was prepared according to protocol [113 (link)]. The protein extracts were resolved by SDS-PAGE, transferred to a PVDF membrane, and incubated with the indicated antibodies. Detections were performed using ECL reagents. The antibodies used are as follows: custom-designed mouse monoclonal antibody against BORIS, mouse monoclonal antibody against CTCF (Santa Cruz, sc-271514), mouse monoclonal antibody against Tubulin (Abcam, ab7291), rabbit polyclonal antibody against SRCAP (Kerafast, ESL103), Rabbit IgG (Abcam, ab37415), rabbit monoclonal against histone H3 (Abcam, ab201456), rabbit monoclonal against LINE-1 ORF1p (Abcam, ab216324), rabbit monoclonal against IRF7 (Thermo Fisher Scientific, SC0617), rabbit polyclonal against LGALS3BP (Thermo Fisher Scientific, PA5-79597), rabbit polyclonal against IFIT1 (Abcam, ab236256), rabbit polyclonal against IFI44 (Thermo Fisher Scientific, PA5-96967), rabbit polyclonal against Aim2 (Cell Signaling, #63660), rabbit polyclonal against OAS1 (MyBioSource, MBS129033).
Pugacheva E.M., Bhatt D.N., Rivero-Hinojosa S., Tajmul M., Fedida L., Price E., Ji Y., Loukinov D., Strunnikov A.V., Ren B, & Lobanenkov V.V. (2024). BORIS/CTCFL epigenetically reprograms clustered CTCF binding sites into alternative transcriptional start sites. Genome Biology, 25, 40.
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5

Antioxidant and Anti-inflammatory Assays

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Anti-Nrf2 antibody (ab31163), anti-heme oxygenase 1 antibody (ab13243), anti-GAPDH antibody ([EPR16891] ab181602), anti-beta tubulin antibody ([EPR16774] ab179513), anti-histone H3 antibody ([EPR17785] ab201456), anti-caspase-3 antibody (ab13847) and anti-Ki67 antibody (ab15580) were purchased from abcam (UK). Other materials were obtained from the following sources: cell counting Kit-8 (DOJINDO, Japan); 5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) (abm, CAT.NO. G492, Applied Biological Materials Inc); qPCR Primer:Nrf2 (cat# RQP051369), GAPDH (cat# RQP049537), HO-1 (cat# RQP048916) were purchased from GeneCopoeia. Inc. (Shanghai,China); BCA protein assay kit (P0012S), reactive oxygen species (ROS, S0033), Hoechst33258 (C1018); cellular glutathione peroxidase assay kit (GSH, S0056), total superoxide dismutase assay kit with WST-8 (SOD, S0101), lipid peroxidation MDA assay kit (MDA, S0131), Annexin V-FITC apoptosis detection kit (Annexin V-FITC, C1063), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were purchased from Beyotime Biotech Co. (Shanghai, China); nuclear and cytoplasmic extraction kit (CW0199S, Beijing, China); and medical linear accelerator (CLINAC 600C/D-SN1214, Varian Medical Systems Inc., USA). RTP was obtained from the Pharmaceutical Laboratory of the Air Force Military Medical University.
Zhang T., Shi L., Li Y., Mu W., Zhang H., Li Y., Wang X., Zhao W., Qi Y, & Liu L. (2020). Polysaccharides extracted from Rheum tanguticum ameliorate radiation-induced enteritis via activation of Nrf2/HO-1. Journal of Radiation Research, 62(1), 46-57.
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6

Western Blot for Osteogenic Markers

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RIPA lysis buffer was used for protein extraction (Beyotime). Protein extracts were electrophoresed on 12% polyacrylamide gels and subsequently transferred onto PVDF membranes (Millipore). Next, 5% nonfat milk in TBST was used to block the membranes; subsequently, they were incubated with the following primary antibodies overnight at 4 °C: BMP4 (ab39973, Abcam), SMAD4 (ab40759, Abcam), ALP (sc-365765, Santa Cruz Biotechnology), collagen I (Col I, ab34710, Abcam), RUNX2 (sc-390351, Santa Cruz Biotechnology), TAZ (ab224239, Abcam), β-actin (cytoplasmic housekeeping, ab8227, Abcam), and Histone H3 (nuclear housekeeping, ab201456, Abcam). Then, membranes were incubated with species-appropriate, horseradish peroxidase (HRP)-conjugated secondary antibodies. An electrochemiluminescence kit was used for protein band visualization (Amersham).
Xie H., Liu M., Jin Y., Lin H., Zhang Y, & Zheng S. (2020). miR-1323 suppresses bone mesenchymal stromal cell osteogenesis and fracture healing via inhibiting BMP4/SMAD4 signaling. Journal of Orthopaedic Surgery and Research, 15, 237.
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