Mbt compass library

Manufactured by Bruker

Sourced in United States

The MBT Compass Library is a database of reference spectra used for identification of microorganisms in the Bruker MALDI Biotyper system. It contains reference data for a wide range of microorganisms, enabling rapid and accurate identification of bacterial and fungal species from clinical samples.

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Lab products found in correlation

Maldi tof ms, by Bruker (3 mentions) Ultraflextreme maldi tof tof, by Bruker (2 mentions) Maldi biotyper compass mbt compass, by Bruker (2 mentions) Bactec fx, by BD (2 mentions) Bactec plus aerobic f, by BD (1 mentions) Maldi sepsityper kit, by Bruker (1 mentions) Bact alert 3d, by bioMérieux (1 mentions) Maldi biotyper, by Bruker (1 mentions) Maldi tof mass spectrometry, by Bruker (1 mentions)

6 protocols using mbt compass library

MALDI-TOF MS identification of Candida auris

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For the MALDI-TOF MS-based identification of C. auris the Bruker MBT Compass Library, Revision E MBT 7854 MSP Library was employed. Samples derived from agar plate were prepared according to the MALDI Biotyper standard protocol (Sauer et al., 2008 (link); Clark et al., 2013 (link); Vlek et al., 2014 (link)).
For species identification from blood cultures, blood culture bottles (BD Bactec Plus Aerobic/F; Becton Dickinson, Heidelberg, Germany) were enriched with 10 ml whole sheep blood and spiked with the respective C. auris strains. The bottles were incubated in a Bactec automated blood culture instrument (Becton Dickinson, Heidelberg, Germany) until they were positive for each isolate. Subsequently, the positive blood cultures were purified by MALDI Sepsityper kit (Bruker Daltonik GmbH, Germany) according to the manufacturer's recommendations (Clark et al., 2013 (link); Lange et al., 2014 (link); Vlek et al., 2014 (link)).

Vatanshenassan M., Boekhout T., Meis J.F., Berman J., Chowdhary A., Ben-Ami R., Sparbier K, & Kostrzewa M. (2019). Candida auris Identification and Rapid Antifungal Susceptibility Testing Against Echinocandins by MALDI-TOF MS. Frontiers in Cellular and Infection Microbiology, 9, 20.

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MALDI-TOF MS for Microbial Identification

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A qualitative analysis of the isolates was performed through MALDI-TOF MS. 5–10 mg of fresh mass from each isolate were placed in 300 μl Milli-Q water and 900 μl ethanol and then vortexed until homogenization. Samples were centrifuged at 14,500 × g for 2 min, pellets were dried at room temperature (RT), and subjected to 50 μl of 70% formic acid and 50 μl of acetonitrile. After a second centrifugation, proteins were obtained in the supernatant, and 1 μl was dried in a MALDI plate subsequently coated with 1 μl of HCCA matrix (α-cyano-4-hydroxycinnamic acid) prepared in a mixture of 50% acetonitrile and 2.5% trifluoroacetic acid. Samples were again dried at RT.
Dried samples were analyzed with MALDI-TOF/TOF “ULTRAFLEXTREME” (Bruker Daltonics, Bremen, Germany) equipment. Obtained spectra were treated with MALDI Biotyper Compass (MBT Compass; Bruker, MA, United States) software, calibrating the spectra before searching and matching, automatizing the measures and obtaining the identifications. Spectra were compared with reference profiles from the MBT Compass Library (Bruker) and finally, score values ≥ 1.70 were considered.

Román-Camacho J.J., García-García I., Santos-Dueñas I.M., Ehrenreich A., Liebl W., García-Martínez T, & Mauricio J.C. (2022). Combining omics tools for the characterization of the microbiota of diverse vinegars obtained by submerged culture: 16S rRNA amplicon sequencing and MALDI-TOF MS. Frontiers in Microbiology, 13, 1055010.

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Salmonella Detection and Characterization

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The blood culture system used was BacT/Alert 3D (bioMérieux) until December 2012, and Bactec FX (Becton Dickinson) after that. Until February 2020 bacterial pathogens in fecal samples were detected using a primary culture-based method, with a PCR-directed culture method using the Amplidiag (Mobidiag Ltd) being used to identify Salmonella in fecal samples after this point. Culture was done using Rappaport broth Salmonella CHROMAgar (CHROMAgar), and xylose-lysine-deoxycholate (XLD) agar. S. enterica isolates were confirmed using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS: Bruker Daltonics, using the Bruker MBT Compass library) and underwent serotyping by slide agglutination with antisera (SSI Diagnostica and Reagensia). Serovars were determined following the White-Kauffmann-Le Minor scheme. Inconclusive cases were sent to the Public Health Agency of Sweden for whole-genome sequencing. Antimicrobial susceptibility testing (AST) was performed using either disk diffusion or gradient tests. Interpretation of the AST results was based on the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines.

Björklund L., Mattisson Y., Bläckberg A., Sunnerhagen T, & Ljungquist O. (2024). A Population-Based Study on the Incidence, Risk Factors, and Outcome of Salmonella Bloodstream Infections in South Sweden 2012–2022. Infectious Diseases and Therapy, 13(3), 501-519.

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Isolation and Identification of E. albertii from Urine Samples

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A total of 48,516 bacterial strains were isolated from patients with fecal and urinary tract infections attending different private hospitals around the Sapporo area in Japan. Among the 35,646 urine-derived strains identified as E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS, MALDI Biotyper; Bruker, Billerica, MA, USA) and MBT Compass Library (ver. 8.0.0, Bruker), we used six strains that were ranked in the top 10 as E. albertii [10 (link)]. Note that fully unlinkable anonymized strains were used in this study.

Fujioka M., Yoshioka S., Ito M, & Ahsan C.R. (2022). Characterization of the Emerging Enteropathogen Escherichia Albertii Isolated from Urine Samples of Patients Attending Sapporo Area Hospitals, Japan. International Journal of Microbiology, 2022, 4236054.

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Blood Culture Identification Protocol

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During the study period guidelines recommended that two sets of blood culture bottles (aerobic and anaerobic) should be taken from two separate venipunctures. The BC system in use was BACTEC FX (BectonDickinson, Franklin Lakes, United States), using a 5-day incubation unless otherwise requested. The main method for species determination was matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS: Bruker Daltonics, using the Bruker MBT Compass library version most recent at the time of sample analysis), with a PCR and 16S sequencing using the Sanger method as a second line method for selected hard-to-identify isolates. Species designations were used according to the 2022 International Code of Nomenclature of Prokaryotes (ICPN) and the List of Prokaryotic names with Standing in Nomenclature (LPSN) [19, 20] (link). The grouping of streptococcal species into the S. mitis group, S. anginosus group, S. sanguinis group, S. salivarius group, S. mutans group, and S. bovis group was done in concordance with previous publications [9, [21] [22] [23] .

Berge A., Lundin J., Bläckberg A., Sunnerhagen T, & Rasmussen M. (2024). Non-betahemolytic streptococcal bacteremia, cardiac implantable electronic device, endocarditis, extraction, and outcome; a population-based retrospective cohort study. Infection.

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MALDI-TOF Yeast Identification Protocol

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A qualitative analysis of yeast isolates was performed with MALDI-TOF mass spectrometry (Bruker Daltonics, Bremen, Germany). A colony from each isolate was placed in 300 μl distilled water and 900 μl ethanol and vortexed until homogenization. Then, samples were pelleted at 13,000 rpm for 2 min, and the pellet was dried at room temperature. Lastly, 50 μl of 70% formic acid and 50 μl of acetonitrile were added to the pellet. Samples were centrifuged for 2 min at 13,000 rpm, obtaining a supernatant with proteins.
About 1 μl of supernatant was dried at room temperature in a matrix-assisted laser desorption/ionization (MALDI) plate, and each sample was coated with 1 L of HCCA matrix (α-cyano-4-hydroxycinnamic acid) prepared in a mixture of 50% acetonitrile and 2.5% trifluoroacetic acid. Samples were again dried at room temperature.
Dried samples were analyzed with MALDI-TOF/TOF “ULTRAFLEXTREME” (Bruker Daltonics, Bremen, Germany) equipment. Generated spectra were treated with MALDI Biotyper compass (MBT Compass; Bruker, Billerica, MA, United States) software, which calibrates the spectra and automatizes the measures and identifications before searching and matching the results. Obtained spectra were compared with reference profiles from the MBT Compass Library (Bruker). Scores ≥2.0 were used as a selection criterion for identifications at species level (Kačániová et al., 2020 (link)).

Carbonero-Pacheco J., Moreno-García J., Moreno J., García-Martínez T, & Mauricio J.C. (2022). Revealing the Yeast Diversity of the Flor Biofilm Microbiota in Sherry Wines Through Internal Transcribed Spacer-Metabarcoding and Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry. Frontiers in Microbiology, 12, 825756.

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