F4679

Manufactured by Merck Group

Sourced in United States, Germany

The F4679 is a laboratory equipment product manufactured by Merck Group. It serves as a key component in various scientific research and analysis processes. The core function of this product is to provide a reliable and precise instrument for specific laboratory applications. No further details on the intended use or additional interpretations are provided.

Automatically generated - may contain errors

Lab products found in correlation

I0634, by Merck Group (1 mentions) L4391, by Merck Group (1 mentions) Diethyldithiocarbamate, by Merck Group (1 mentions) A7250, by Merck Group (1 mentions)

4 protocols using f4679

Collagen Membrane-Loaded FK506 Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

FK506 (F4679, Sigma-Aldrich, St. Louis, USA), supplied as a powder (5 mg) in a glass bottle, was used in this study. The powder was dissolved with 1 mL of normal saline (20 mL, Choongwae Pharm, Korea) before application. The concentration of FK506 (0.5 mg/0.1 mL) was selected based on previous published studies [27 (link), 28 (link)]. Collagen membrane (OSSGUIDE®, Bioland Co., Cheongju, Korea, 15 mm × 20 mm × 0.2 mm) was cut into 10 pieces, with each piece having a size of 5 mm × 5 mm × 0.2 mm. The membranes (n = 10) were hydrated with 0.1 mL of FK506 solution (0.5 mg/0.1 mL) (Fig. 1).
Fig. 1

Preparation of FK506. A FK506 monohydrate. B Collagen membrane loaded with FK506

Kim J.H., Choi Y.J., Park H.I, & Ahn K.M. (2022). The effect of FK506 (tacrolimus) loaded with collagen membrane and fibrin glue on promotion of nerve regeneration in a rat sciatic nerve traction injury model. Maxillofacial Plastic and Reconstructive Surgery, 44(1), 14.

+ Open protocol

+ Expand

Immortalized Mouse Podocyte Cell Line

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The conditionally immortalized mouse podocyte cell line was kindly donated by Professor Shengqiang Yu from Shanghai Changzheng Hospital. Briefly, podocytes were cultured as previously shown.²¹ (link) Podocytes were starved overnight before experiments, and treated with LPS (20 μg/ml, L4391, Sigma), CsA (5 μg/ml, C1832, Sigma), FK506 (10 μg/ml, F4679, Sigma), TAK-242(1 μM, HY-11109, MCE), Ionomycin (0.5 μM, I0634, Sigma) or PDTC (20 μM, S1808, Beyotime) for indicated time. Mouse Glomerular Endothelial cells (mGEnC) was provided by Professor Chuanming Hao at Ruijin Hospital, and were cultured in RPMI 1640 medium with 10% FBS in a humidified atmosphere of 5% CO₂. Human Mesangial Cells (HMC) was bought from ATCC, and was cultured in DMEM medium with 10% FBS in a humidified atmosphere of 5% CO₂.

Shen X., Weng C., Wang Y., Wang C., Feng S., Li X., Li H., Jiang H., Wang H, & Chen J. (2020). Lipopolysaccharide-induced podocyte injury is regulated by calcineurin/NFAT and TLR4/MyD88/NF-κB signaling pathways through angiopoietin-like protein 4. Genes & Diseases, 9(2), 443-455.

+ Open protocol

+ Expand

Prion Peptide Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Synthetic prion peptides PrP (106-126) (sequence, Lys-Thr-Asn-Met-Lys-His-Met-Ala-Gly-Ala-Ala-Ala-Ala-Gly-Ala-Val-Val-Gly-Gly-Leu-Gly) and scrambled PrP (106-126) (sequence, Asn-Gly-Ala-Lys-Ala-Leu-Met-Gly-Gly-His-Gly-Ala-Thr-Lys-Val-Met-Val-Gly-Ala-Ala-Ala) were synthesized by Peptron (Seoul, Korea) [20 (link)]. The PrP peptides were dissolved in sterile dimethyl sulfoxide (DMSO) at a concentration of 10 mM (stock) and stored at -20°C.
The stock solution of FK506 (10 mM; F4679, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in DMSO. The stock solution of N-acetyl-L-cysteine (NAC, 1 M; A7250, Sigma-Aldrich), diethyldithiocarbamate (DDC, 100 mM; Sigma-Aldrich), and 3-amino-1,2,4-triazole (AT, 500 mM; Sigma-Aldrich) was dissolved in distilled water.

Moon J.H., Hong J.M, & Park S.Y. (2021). Calcineurin Activation by Prion Protein Induces Neurotoxicity via Mitochondrial Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity, 2021, 5572129.

+ Open protocol

+ Expand

mRMEC Hypoxia and FK506 Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse (m)RMEC culture. The mRMECs (cat. no. CD-1065) were purchased from Cell Biologics, Inc. (Chicago, IL, USA). The mRMECs were cultured in a 100 mm collagen-coated culture dish containing 10 ml supplemented medium (RPMI-1640; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) with 20% fetal bovine serum (16000044; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and maintained at 37˚C in a humidified atmosphere of 5% CO 2 and 95% air. The culture medium was replaced every 2 days. The cells used in the present study were between passages 3 and 4. When the cells had grown to 70% confluence, they were cultured under hypoxic conditions (93% N 2 , 5% CO 2 , 2% O 2 ) at 37˚C for 24 h. Then cells were then washed twice with phosphate-buffered saline (PBS) and then exposed to FK506 (F4679; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at different concentrations (0.1, 1 and 10 µM) for 24 and 48 h, respectively. FK506 was dissolved in dimethyl sulfoxide (<0.1%), which caused no deleterious effect on the viability of the mRMECs in preliminary experiments.

Hu Y., Yang B., Xu Y., Jiang L., Tsui C.K, & Liang X. (2017). FK506 suppresses hypoxia‑induced inflammation and protects tight junction function via the CaN‑NFATc1 signaling pathway in retinal microvascular epithelial cells. Molecular medicine reports, 16(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!