Transponders

Manufactured by Avidity Science

Transponders are compact electronic devices that transmit and receive wireless signals. They serve as identification and communication modules in various applications.

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Lab products found in correlation

Female hartley guinea pigs, by Charles River Laboratories (2 mentions) Dnp ige, by Merck Group (1 mentions) Female hartley strain guinea pigs, by Charles River Laboratories (1 mentions)

Spelling variants of this product

IPTT-300 transponder (12 citations) IPTT-300 temperature transponders (9 citations) BMDS IPTT-300 transponders (5 citations) Microchip transponders (3 citations) Implantable subcutaneous temperature transponder (3 citations) Temperature transponder (3 citations) DAS-8007 transponder reader (3 citations) Subcutaneous transponder chip (2 citations)

4 protocols using transponders

Guinea Pig Study of Orthopoxvirus Infection

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Female Hartley Guinea pigs (250–325 g; 5–8 weeks of age) (Charles River Laboratories) were obtained and housed in compliance with a protocol (ASP# 2022-024-E) approved by the Rocky Mountain Laboratories Animal Care and Use committee. To monitor temperatures, transponders (Bio Medic Data Systems, Inc., Seaford, DE) were implanted subcutaneously. 100 PFU were delivered via intradermal injection and temperature recorded daily for 20 days post infection. At 30 days post infection, sera were collected for antibody titration and animals were euthanized.
To determine replication in Guinea pigs, animals were inoculated intradermally with a dose of 100 PFUs each of Sheila Smith, Iowa, and Iowa-pRapL. Spleens were harvested from groups of 3 GP/day on days 3, 6, and 9 post-infection. Individual spleens were weighed and homogenized for determination of PFUs and genome equivalents.

Nock A.M., Aistleitner K., Clark T.R., Sturdevant D., Ricklefs S., Virtaneva K., Zhang Y., Gulzar N., Redekar N., Roy A, & Hackstadt T. (2023). Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters. PLOS Pathogens, 19(7), e1011527.

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Investigating IL-10's Role in Passive Anaphylaxis

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WT and IL-10^−/− were sensitized i.v. with 10 µg DNP-IgE (clone SPE7, Sigma). 24h later, they were challenged i.v. with 100 µg of DNP-BSA, and changes in core body temperature were recorded using subcutaneously placed transponders (Biomedic Data Systems). To assess the effects of IL-10 on the development of passive anaphylaxis, some mice were injected i.p. with 3 µg rIL-10 starting 5 hours before injection with DNP-IgE, followed by 4 µg rIL-10 i.v. concurrently with the DNP-IgE injection. A final dose of 3 µg rIL-10 was administered i.p. one hour before challenge with DNP-BSA.

Polukort S.H., Rovatti J., Carlson L., Thompson C., Ser-Dolansky J., Kinney S.R., Schneider S.S, & Mathias C.B. (2016). IL-10 enhances IgE-mediated mast cell responses and is essential for the development of experimental food allergy in IL-10-deficient mice. Journal of immunology (Baltimore, Md. : 1950), 196(12), 4865-4876.

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Rickettsia rickettsii Infection in Guinea Pigs

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Female Hartley strain guinea pigs (350 g) were purchased from Charles River Laboratories (MA) and housed in an animal biosafety level 3 laboratory under a protocol approved by the Rocky Mountain Laboratories Animal Care and Use Committee. Guinea pigs were implanted with transponders (Bio Medic Data Systems, Inc., Seaford, DE) to monitor temperatures. R. rickettsii strains Sheila Smith, Iowa, Iowa::pRAMF2-SS-RARP-2, and Iowa::pRAMF2-MTase were inoculated intradermally with 100 PFU. Temperatures were monitored for 16 days after infection. Animals were euthanized on day 28, and sera were collected for antibody titration.

Lehman S.S., Noriea N.F., Aistleitner K., Clark T.R., Dooley C.A., Nair V., Kaur S.J., Rahman M.S., Gillespie J.J., Azad A.F, & Hackstadt T. (2018). The Rickettsial Ankyrin Repeat Protein 2 Is a Type IV Secreted Effector That Associates with the Endoplasmic Reticulum. mBio, 9(3), e00975-18.

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Guinea Pig MPXV Intradermal Infection

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Female Hartley guinea pigs (300 to 500 g; 5 to 8 weeks of age) (Charles River Laboratories) were obtained and housed in compliance with a protocol (2017-064.2) approved by the Rocky Mountain Laboratories Animal Care and Use committee. To monitor temperatures, transponders (Bio Medic Data Systems, Inc., Seaford, DE) were implanted subcutaneously. One hundred PFU was delivered via intradermal injection, and temperature was recorded daily for 16 days postinfection. At 28 days postinfection, sera were collected for antibody titration and animals were euthanized.

Nock A.M., Clark T.R, & Hackstadt T. (2022). Regulator of Actin-Based Motility (RoaM) Downregulates Actin Tail Formation by Rickettsia rickettsii and Is Negatively Selected in Mammalian Cell Culture. mBio, 13(2), e00353-22.

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