Oxylet le 400 air supplier

The amount of food and water consumed every 24 h was individually recorded in metabolic cages (Oxylet LE 1305 Physiocage, Panlab, Panlab Harvard, Barcelona, Spain). For this purpose, female rats were placed in individual acrylic cages in temperature-controlled environment (23 ± 2°C) with a 12 h : 12 h photoperiod (lights on at 07:00 h). Rats were acclimatized in the cages for 24 h before experimental measurements. Oxygen consumption (VO₂) and carbon dioxide production (VCO₂) were measured every 12 min during 24 h using an O₂ and CO₂ analyzer (Oxylet LE 405-gas analyzer, Panlab) at a controlled flow rate of 900 ml/min (Oxylet LE 400-air supplier, Panlab). At each point, we used the software v3.0.01 metabolism (Panlab Harvard Apparatus, Barcelona, Spain) for the quantification of respiratory quotient (RQ) based on the VCO₂/VO₂ ratio and energy expenditure (EE) in kcal/day. During all procedures, rats had free access to control and experimental diets and water ad libitum. Locomotor activity as well as food and water intake was measured by continuous recording using the Panlab weight transducer (LE1305 sensor platform, Panlab).

Indirect calorimetry analyses were performed in sham and PCA rats using an OxyletPro System (Panlab Harvard Apparatus, Barcelona, Spain). For this purpose, the animals were placed in individual acrylic cages (Oxylet LE 1305 Physiocage, PANLAB) in a controlled temperature environment (23 ± 2°C) with a photoperiod of 12 h:12 h (lights on at 07:00 h). At week 12 after surgery, a set of 6 rats was acclimated in the Oxylet cages for 48 h before experimental measurements. Oxygen consumption (VO₂) and carbon dioxide production (VCO₂) were measured every 12 min for 72 h by an O₂ and CO₂ analyzer (Oxylet LE 405-gas analyzer, PANLAB) at a controlled flow rate of 900 ml/min (Oxylet LE 400-air supplier, PANLAB). At each point of analysis, the Metabolism v3.0.01 software (Panlab Harvard Apparatus, Barcelona, Spain) automatically calculated the respiratory quotient (RQ) as the VCO₂/VO₂ ratio and energy expenditure (EE) in kcal/day/kg^0.75as VO₂ × 1.44 × [3.815 + (1.232 × RQ)], according to the Weir formula [20 (link)]. During all procedures, rats had access to food and water ad libitum. Activity and food and drink intake were also measured by a continuous recording using Panlab's weight transducer (LE1305 sensor platform, Panlab).