Mda mb 231

The MCF10A (cat. no. CL-0525) cell line was purchased from Wuhan Procell Company, derived from human normal mammary epithelial cells, and was cultured in specialized MCF10A medium (Procell Life Science & Technology Co., Ltd.). The human BC cell lines, SKBR3 (cat. no. 1101HUM-PUMC000085), MCF7 (cat. no. 1101HUM-PUMC000013) and Hs578T (cat. no. 1101HUM-PUMC000670), were cultured in DMEM (Dalian Meilun Biology Technology Co.) supplemented with 10% fetal bovine serum (Shanghai ExCell Biological Products Co., Ltd.), 100 U/ml penicillin and 100 µg/ml streptomycin (Dalian Meilun Biology Technology Co., Ltd.) in a 5% CO₂ incubator at 37°C, while the MDA-MB-231 (cat. no. 1101HUM-PUMC000014) and MDA-MB-468 cells (cat. no. 1101HUM-PUMC000249) were cultured in RPMI-1640 medium (Dalian Meilun Biology Technology Co.). All the human BC cell lines were purchased from the ChineseNational Infrastructure of Cell line Resource (NICR).

The human normal mammary epithelial cell line, MCF10A (cat no. CL-0525), was purchased from Wuhan Procell Company and cultured in MCF10A special medium (Procell Co., Ltd.). The human BC cell lines, SKBR3 (cat no. 1101HUM-PUMC000085), MCF7 (cat no: 1101HUM-PUMC000013), MDA-MB-231 (cat no. 1101HUM-PUMC000014) and Hs578T (cat no. 1101HUM-PUMC000670) and MDA-MB-468 (cat no. 1101HUM-PUMC000249), were purchased from the National Infrastructure of Cell line Resource (NICR). The SKBR3, MCF7, MDA-MB-231 and Hs578T cells were cultured in DMEM (Meilun Biotech Co., Ltd.) and the MDA-MB-468 cells were cultured in RPMI-1640 (Meilun Biotech Co., Ltd.) medium supplemented with 10% fetal bovine serum (HyClone; Cytiva), 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) in a 5% CO₂ incubator at 37°C.

The
human gastric cancer cell line MGC
803, breast cancer cell line MDA-MB-231, and ovarian carcinoma cell
line SKOV-3 were obtained from Cell Bank, Type Culture Collection
of Chinese Academy of Sciences (Shanghai, People’s Republic
of China). The human lung cancer cell A549 and hepatocellular cancer
cell HepG 2 were gifts from Professor Tao Wu (Shanghai University
of Traditional Chinese Medicine). The MDA-MB-231 and HepG 2 cells
were cultured in DMEM medium (Meilunbio, People’s Republic
of China). MGC 803 and A549 cells were cultured in RPMI 1640 medium
(Meilunbio). SKOV-3 cells were cultured in McCoy’s 5A medium
(Gibco). The medium contained 10% heat-inactivated fetal bovine serum
(Gibco), 100 units/mL penicillin, and 100 μg/mL streptomycin
(Gibco). Cells were incubated at 37 °C in a humidified atmosphere
of 5% CO₂.