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Acuity uplc system

Manufactured by Waters Corporation
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The Acuity UPLC system is a high-performance liquid chromatography (HPLC) instrument manufactured by Waters Corporation. It is designed for the separation, identification, and quantification of chemical compounds in a variety of applications. The Acuity UPLC system utilizes ultra-high pressure liquid chromatography (UPLC) technology to achieve enhanced resolution, sensitivity, and speed compared to traditional HPLC methods.

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Lab products found in correlation

Ltq orbitrap xl mass spectrometer, by Thermo Fisher Scientific (2 mentions) Aluminum chloride, by Merck Group (1 mentions) Ferrozine, by Merck Group (1 mentions) Diaion hp 20, by Merck Group (1 mentions) Fluoresceine, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Folin ciocalteu reagent, by Loba Chemie (1 mentions) 2 2 diphenyl 1 picrylhydrazyl dpph 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid abts 2 4 6 tris 2 pyridyl s triazine tptz, by Merck Group (1 mentions) Tripletof 5600, by AB Sciex (1 mentions) Sep pak c18 cartridge, by Waters Corporation (1 mentions) Qtrap 5500, by AB Sciex (1 mentions) Qtrap 4500, by AB Sciex (1 mentions) Tripletof 6600, by AB Sciex (1 mentions) Acquity uplc peptide beh c18 column, by Waters Corporation (1 mentions) Tqd quantum triple quadrupole mass spectrometer, by Waters Corporation (1 mentions) Van guard beh c18 column, by Waters Corporation (1 mentions) Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions) Avance 3 hd 500 mhz nmr spectrometer, by Bruker (1 mentions) Xcalibur 2, by Thermo Fisher Scientific (1 mentions) Omnic software, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

UPLC system (190 citations) Acuity UPLC (2 citations) Acuity I-class UPLC system (2 citations)

7 protocols using acuity uplc system

1

Phytochemical Extraction and Characterization

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Diaion
HP-20, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis
(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), ascorbic acid, fluoresceine, ferrozine,
aluminum chloride, and all other chemicals were purchased from Sigma-Aldrich,
(Schnelldorf, Germany). Rutin and gallic acid were purchased from
Sigma Chemical Co. (St. Louis, Mo, USA). The Folin–Ciocalteu
reagent was from Loba-Chemie (Mumbai, India). Propylene glycol monocaprylate
(Capryol 90) and diethylene glycol monoethyl ether (Transcutol P)
were kindly supplied by Gattefossé (Saint Priest, France).
LC–MS analysis was carried out on a Waters Acuity UPLC system,
and all spectrophotometric analyses were conducted on microplate reader
BMG Labtech FluoStar Omega (Ortenberg, Germany). All solvents used
for extraction were of analytical grade, while solvents used for LC–MS
analysis were of HPLC-mass grade.
Nematallah K.A., Elmekkawy S., Abdollah M.R., Elmazar M.M., Abdel-Sattar E, & Meselhy M.R. (2022). Cheminformatics Application in the Phytochemical and Biological Study of Eucalyptus globulus L. Bark as a Potential Hepatoprotective Drug. ACS Omega, 7(9), 7945-7956.
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2

High-Resolution LC-MS/MS Analysis of Compounds

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High-resolution LC–MS–MS
was carried out on an Acuity UPLC system (Waters) equipped with an
HSS T3 column (100 × 1.0 mm, particle size 1.8 μm; Waters).
The mobile phase was composed of two solvents: 0.1% formic acid in
water (A), 99.9% acetonitrile, and 0.1% formic acid (B), at a flow
rate of 150 μL/min. The binary gradient of the mobile phase
was 0–1 min, 5% B; 1–16 min 5–95% B; 16–18
min, 95% B; 18–20, 5% B. EE was dissolved in 5% acetonitrile
with a concentration of 1 mg/mL and then filtered using a syringe
filter with a pore size 0.2 μm. The sample injection volume
was 3.1 μL (full loop injection). Eluted compounds were detected
from m/z 100 to 1000 with a resolution of 100,000
in the negative-ion mode using the following instrument settings:
nebulizer gas, nitrogen, 1.6 bar; dry gas, nitrogen, 6 liters min–1, 190 °C; capillary, −5500 V; in-source
CID energy, 0 V; hexapole RF, 100 Vpp; quadrupole ion energy, 5 eV;
collision gas, argon; collision energy, 10 eV; collision RF 200/400
Vpp (timing 50/50); transfer time, 70 μs; prepulse storage,
5 μs; pulser frequency, 10 kHz; spectra rate, 3 Hz. Internal
mass calibration was performed by the infusion of 20 μL of 10
mM lithium formate in isopropanol: water, 1:1 (v/v), at a gradient
time of 18 min using a diverter valve.
Nematallah K.A., Elmekkawy S., Abdollah M.R., Elmazar M.M., Abdel-Sattar E, & Meselhy M.R. (2022). Cheminformatics Application in the Phytochemical and Biological Study of Eucalyptus globulus L. Bark as a Potential Hepatoprotective Drug. ACS Omega, 7(9), 7945-7956.
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3

Fecal Lipidomic Profiling of Mice

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Fecal lipidomic profiling was performed at the Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, as described previously (62 (link)). Briefly, collected mouse feces were homogenized in methanol, and lipids were obtained by single-phase extraction. Lipids were separated on an Acquity UPLC Peptide BEH C18 column (Waters). Oxidized fatty acids, including LA metabolites, were extracted using a Sep-Pak C18 cartridge (Waters) and analyzed by LC-quadrupole MS (QTRAP4500 or QTRAP5500; Sciex). Untargeted lipidomics was performed using an ACUITY UPLC system (Waters) coupled with QTOF-MS (TripleTOF 5600+ or TripleTOF 6600; Sciex) (93 (link)).
Miki Y., Taketomi Y., Kidoguchi Y., Yamamoto K., Muramatsu K., Nishito Y., Park J., Hosomi K., Mizuguchi K., Kunisawa J., Soga T., Boilard E., B. Gowda S.G., Ikeda K., Arita M, & Murakami M. (2022). Group IIA secreted phospholipase A2 controls skin carcinogenesis and psoriasis by shaping the gut microbiota. JCI Insight, 7(2), e152611.
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4

UPLC-MS Quantification of Scu in Pharmacokinetics

Cited 1 time
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The UPLC–MS
measurement for SCU was reported by Li et al.31 (link) An Acuity UPLC system equipped with a binary pump, degasser, autosampler,
and temperature-controlled column compartment and a TQD quantum triple-quadrupole
mass spectrometer equipped with an electrospray ionization (ESI) source
(Waters Corp., Manchester, UK) were used for SCU analysis in the pharmacokinetics
and tissue distribution studies. Liquid chromatography (LC) was performed
by a Waters Van Guard BEH C18 column (2.1 mm × 50 mm, 1.7 μm)
at 45 °C with the mobile phase consisting of 0.1% formic acid
in acetonitrile (A) and 0.1% formic acid water (B). The gradient program
is as follows: 0–0.5 min, 5% A and 95% B; 0.5–3 min,
5–95% A and 95–5% B; and 3–3.5 min, 95–5%
A and 5–95% B. The peaks were obtained at a flow rate of 0.3
mL/min with a sample injection volume of 1 μL. The electrospray
positive ionization (ESI+) was used for detection and analysis.
In the positive ion mode, the SCU parameters are as follows: capillary
voltage at 3 kV, cone voltage at 30 V, and collision energy at 20
eV; the puerarin parameters are as follows: capillary voltage at 3
kV, cone voltage at 40 V, and collision energy at 30 eV. SCU and puerarin
(internal standard) were quantified using the selected ion recording
mode (SIR) of their parent ions, 463 and 417, respectively.
Yang C., Zhao Q., Yang S., Wang L., Xu X., Li L, & Al-Jamal W.T. (2022). Intravenous Administration of Scutellarin Nanoparticles Augments the Protective Effect against Cerebral Ischemia–Reperfusion Injury in Rats. Molecular Pharmaceutics, 19(5), 1410-1421.
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5

Analytical Techniques for Compound Characterization

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Melting points of compounds were obtained on a Global Medical and Lab Solutions melting point apparatus (India). Optical rotations were measured on a JASCO P-1010 polarimeter (JASCO Inc. Easton, MD, USA). IR spectra were acquired on an FTIR instrument (Nicolet iS50 FT-IR spectrometer, Thermo Fisher Scientific, Waltham, MA, USA). The IR data were analyzed with OMNIC software (Thermo Fisher Scientific). A Bruker 500 MHz Avance III HD NMR spectrometer (Bruker, Billerica, MA, USA) was used to obtain NMR spectra of the synthesized compounds in this study. The NMR data were analyzed with Topspin software (Bruker). Liquid chromatography–high-resolution mass spectrometry (LC-HRMS) data were acquired on an Acuity UPLC system (Waters, Milford, MA, USA) connected to an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific). The UPLC was equipped with a photo diode array (PDA) detector to analyze the UV–vis spectra (wavelength range from 190 to 500 nm) of select compounds shown in the Supporting Information (SI, Part 11). The LCHRMS data were analyzed using Xcalibur 2.1 software (Thermo Scientific). Chem3D 20.1.1 (PerkinElmer, Waltham, MA, USA) was used to perform the structural overlays in Figure 12.
Varela K., Al Mahmud H., Arman H.D., Martinez L.R., Wakeman C.A, & Yoshimoto F.K. (2022). Autoxidation of a C2-Olefinated Dihydroartemisinic Acid Analogue to Form an Aromatic Ring: Application to Serrulatene Biosynthesis. Journal of natural products, 85(4), 951-962.
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6

Optical and Spectroscopic Analysis

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A Laxco™ POL-300 Series Digital Automatic Polarimeter (Thermo Fisher Scientific, Waltham, MA) was used for optical rotation measurements. A 500 MHz Bruker (Billerica, MA) NMR spectrometer was used to obtain NMR spectra. LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA) connected to a Waters Acuity UPLC system (Milford, MA) was used to acquire high resolution mass spectrometry data.
Chung E., Offei S.D., Jia U.T., Estevez J., Perez Y., Arman H.D, & Yoshimoto F.K. (2021). A synthesis of a rationally designed inhibitor of cytochrome P450 8B1, a therapeutic target to treat obesity. Steroids, 178, 108952.
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7

Automated HILIC Glycopeptide Isolation

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Isolation of intact glycopeptides was conducted on a Waters Acuity UPLC system (Waters, USA) equipped with a homemade HILIC (hydrophilic-interaction chromatography) column (click maltose52 , 5 µm, 100 Å) according to our recently established automated method53 (link). Briefly, peptides digested from 5 µL serum were dissolved in 35 µL 80% ACN/1% TFA and loaded onto HILIC column. Mobile phase A and B were 0.1% TFA in H2O and 98% ACN/0.1% TFA, respectively. The gradient was kept at 80% mobile phase B for 11 min and then dropped directly to 30% mobile phase B and maintained for 5 min. Intact glycopeptides were collected during 12–13 min of the gradient. The sample was dried for LC–MS/MS analysis.
Fang Z., Qin H., Mao J., Wang Z., Zhang N., Wang Y., Liu L., Nie Y., Dong M, & Ye M. (2022). Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation. Nature Communications, 13, 1900.
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