Gentamycin

Manufactured by Serva Electrophoresis

Sourced in Germany, France

Gentamycin is a reagent used in electrophoresis applications. It is a biochemical compound that aids in the separation and analysis of macromolecules such as proteins, nucleic acids, and other biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (4 mentions) Hct116, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Biosera (2 mentions) Rpmi 1640 medium, by Biosera (2 mentions) Fetal bovine serum (fbs), by Biowest (2 mentions) Mda mb 231, by European Collection of Authenticated Cell Cultures (1 mentions) Mcf 7, by European Collection of Authenticated Cell Cultures (1 mentions) Mem non essential amino acids solution, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Fbs 12a, by Capricorn (1 mentions) Restriction endonuclease, by Thermo Fisher Scientific (1 mentions) Dna ligase, by Thermo Fisher Scientific (1 mentions) Kanamycin, by Serva Electrophoresis (1 mentions) Rifampicin, by Serva Electrophoresis (1 mentions) 5 hydroxyectoine, by Merck Group (1 mentions) Ampicillin, by Carl Roth (1 mentions) Mda mb 231 cells, by European Collection of Authenticated Cell Cultures (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Hek293t crl 3216, by American Type Culture Collection (1 mentions)

9 protocols using gentamycin

Cell Culture Conditions for Cancer Cell Lines

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The human cervical carcinoma HeLa, human
ovarian carcinoma A2780, and human breast adenocarcinomas MDA-MB-231
and MCF-7 were purchased from the European collection of authenticated
cell cultures ECACC (Salisbury, UK), human monocytic cell line Thp-1
was purchased from the American Type Culture Collections (ATCC), human
colon carcinoma HCT-116 was kindly provided by Dr. M. Brazdova, Institute
of Biophysics (Brno, CZ), and cisplatin-resistant human ovarian carcinoma
A2780cisR (a cisplatin-resistant variant of A2780 cells) was kindly
provided by Professor B. Keppler, University of Vienna (Austria).
A2780 and A2780cisR cells were grown in RPMI-1640 medium (Biosera,
Boussens, France) supplemented with gentamycin (50 μg mL^–1, Serva, Heidelberg, Germany) and 10% heat-inactivated
FBS (Biosera). The other cells were grown in DMEM medium (high glucose
4.5 g L^–1, PAA) supplemented with gentamycin (50
μg mL^–1, Serva) and 10% heat-inactivated FBS
(Biosera). The cells were cultured in a humidified incubator at 310
K in a 5% CO₂ atmosphere and subcultured 2–3 times
a week with a desired plating density.

Kostrhunova H., McGhie B.S., Markova L., Novakova O., Kasparkova J., Aldrich-Wright J.R, & Brabec V. (2023). Platinum(IV) Derivatives of [Pt(1S,2S-diaminocyclohexane)(5,6-dimethyl-1,10-phenanthroline)] with Diclofenac Ligands in the Axial Positions: A New Class of Potent Multi-action Agents Exhibiting Selectivity to Cancer Cells. Journal of Medicinal Chemistry, 66(12), 7894-7908.

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Cell Culture and Bacterial Propagation

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The human ovarian carcinoma cells A2780 (ECACC) were cultured in RPMI-1640 medium supplemented with 10% of heat-inactivated fetal bovine serum (FBS, Biosera LTD, Ringmer, U. K.) and gentamycin (50 μg mL -1 , Serva). The CHO-K1 and MMC-2 (Chinese hamster ovary cells) were grown in DMEM medium (high glucose, 4.5 g L -1 , Biosera) supplemented with gentamycin (50 μg mL -1 , Serva) and 10% heat-inactivated fetal bovine serum (Biosera). The A2780 cells were kindly supplied by prof. Keppler (Vienna, Austria), the CHO-K1 cell line (wild type) and its mutant cell line MMC-2 were kindly supplied by Dr. M. Pirsel, Cancer Research Institute, Slovak Academy of Sciences (Bratislava, Slovakia). The cells were cultured in a humidified incubator at 37 °C in a 5% CO 2 atmosphere and subcultured 2-3 times a week with an appropriate plating density. Escherichia coli (CCM 7929) was obtained from the Czech Collection of Microorganisms (CCM), Masaryk University, Faculty of Science, Brno, Czech Republic as a freeze-dried pellet. The cells were rehydrated and propagated on Luria broth (LB) agar plates according to the supplier protocol. Microbiological experiments were performed under standard sterile conditions.

Kasparkova J., Kostrhunova H., Novohradsky V., Pracharova J., Curci A., Margiotta N., Natile G, & Brabec V. (2017). Anticancer kiteplatin pyrophosphate derivatives show unexpected target selectivity for DNA. Dalton transactions (Cambridge, England : 2003), 46(41).

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CaCo-2 and HEK293T cell cultivation

Cited 1 time

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CaCo-2 cells (kindly provided by Konstantin Sparrer, University Hospital Ulm, Germany) were cultivated in DMEM (Dulbecco’s Modified Eagle Medium DMEM; 11500516, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS-12A; Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM Gluta-MAX™ (35050061, Thermo Fisher Scientific), 25 mM HEPES (15630080, Thermo Fisher Scientific), 1× MEM Non-Essential Amino Acids Solution (11140050, Thermo Fisher Scientific), and 50 µg/mL gentamycin (1405-41-0, Serva Electrophoresis, Heidelberg, Germany) and passaged every 2–3 days depending on confluence.
HEK293T T7/N cells (previously described in [19 (link)]) were cultivated in DMEM (Dulbecco’s Modified Eagle Medium DMEM; 11500516, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated FBS, 2 mM Gluta-MAX™, 25 mM HEPES, 5 µg/mL blasticidin (asnt-bl-1, InvivoGen, San Diego, CA, USA), and 2 µg/mL puromycin (SC-1080713, Santa Cruz Biotechnology, Dallas, TX, USA).
All cells were incubated at 37 °C, 5% CO₂, and 80% relative humidity.

Cordsmeier A., Jungnickl D., Herrmann A., Korn K, & Ensser A. (2023). Analysis of SARS-CoV-2 Spike Protein Variants with Recombinant Reporter Viruses Created from a Bacmid System. International Journal of Molecular Sciences, 24(9), 8156.

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Antibiotics and Chemicals Used in Research

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The antibiotics gentamycin, rifampicin, and kanamycin were obtained from Serva (Heidelberg, Germany); ampicillin was purchased from Carl Roth GmbH (Karlsruhe, Germany). Anhydrotetracycline hydrochloride, desthiobiotin, and Strep-Tactin Superflow chromatography material were obtained from IBA GmbH (Göttingen, Germany). Marker proteins for size exclusion chromatography experiments were purchased from Sigma-Aldrich (Taufkirchen, Germany). Restriction endonucleases and DNA ligase were obtained from ThermoScientific (St. Leon-Rot, Germany) and used as suggested by the manufacturer. Ectoine was a kind gift from the bitop AG (Witten, Germany) and 5-hydroxyectoine was purchased from Merck (Darmstadt, Germany). γ-ADABA was purchased from abcr GmbH (Karlsruhe, Germany).

Hermann L., Dempwolff F., Steinchen W., Freibert S.A., Smits S.H., Seubert A, & Bremer E. (2021). The MocR/GabR Ectoine and Hydroxyectoine Catabolism Regulator EnuR: Inducer and DNA Binding. Frontiers in Microbiology, 12, 764731.

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Cell Lines and Culture Conditions

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The human rhabdomyosarcoma (RD) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), human colorectal carcinoma cells HCT116 and human breast cancer MCF-7 cells were kindly supplied by Professor B. Keppler, University of Vienna (Austria), human melanoma 518A2 were kindly supplied by prof. R. Schobert, University of Bayreuth (Germany). In addition, highly invasive breast carcinoma MDA-MB-231 cells and human MRC5pd30 cells derived from normal lung tissue were purchased from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, UK). RD cells were grown in RPMI 1640 medium (Biosera, Boussens, France), the other cells were kept in DMEM medium (high glucose, 4.5 gL⁻¹, PAA, Pasching, Austria); both media were supplemented with gentamycin (50 mgmL⁻¹, Serva, Heidelberg, Germany) and 10% heat-inactivated fetal bovine serum (PAA, Pasching, Austria). All cell lines were cultured in a humidified atmosphere (5% CO₂) at 37°C and subcultured two to three times a week.

Hreusova M., Novohradsky V., Markova L., Kostrhunova H., Potočňák I., Brabec V, & Kasparkova J. (2022). Gallium(III) Complex with Cloxyquin Ligands Induces Ferroptosis in Cancer Cells and Is a Potent Agent against Both Differentiated and Tumorigenic Cancer Stem Rhabdomyosarcoma Cells. Bioinorganic Chemistry and Applications, 2022, 3095749.

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Centriole Analysis of Transfected Cells

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Cells were maintained in a humidified atmosphere containing 5% CO2 at 37 °C and subcultured every two to three days. Culture medium consisted of DMEM/F12 supplemented with GlutaMAX, 4.5 mg/mL D-glucose (high glucose) (Thermo Fisher, Waltham, MA, USA/Gibco #31331-093), 10% fetal bovine serum (FBS, Biowest, Nuaillé, France), and 50 mg/mL gentamycin (Serva, Heidelberg, Germany). For transfection, cells were seeded on 12 mm glass coverslips in a 24-well plate at a density of 10,000 (C2C12) or 50,000 cells (ARPE-19)/well. For centriole analysis, cells were fixed 24 h after transfection.

Steinfeldt J., Becker R., Vergarajauregui S, & Engel F.B. (2021). Alternative Splicing of Pericentrin Contributes to Cell Cycle Control in Cardiomyocytes. Journal of Cardiovascular Development and Disease, 8(8), 87.

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Culturing Human and Murine Cell Lines

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Human embryonic kidney epithelial cells (HEK 293T, CRL-3216, ATCC, Manassas, VA, USA), HeLa cells (ATCC) and murine embryonic fibroblasts (own repository of primary cell cultures) were cultivated at 37 °C, 5% CO₂ and 80% humidity using Dulbecco’s modified Eagle medium (DMEM, 11960044, Thermo Fisher Scientific, Waltham, MA, USA). Cell culture medium was supplemented with 1× GlutaMAX^TM (35050038, Thermo Fisher Scientific), 10 μg/mL gentamycin (22185.03, SERVA, Heidelberg, Germany) and 10% fetal bovine serum (FBS, F7524, Sigma Aldrich, St. Louis, MO, USA).

Häge S., Sonntag E., Borst E.M., Tannig P., Seyler L., Bäuerle T., Bailer S.M., Lee C.P., Müller R., Wangen C., Milbradt J, & Marschall M. (2020). Patterns of Autologous and Nonautologous Interactions between Core Nuclear Egress Complex (NEC) Proteins of α-, β- and γ-Herpesviruses. Viruses, 12(3), 303.

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MTT Assay for Metallohelices Cytotoxicity

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HCT116 cells were obtained from prof. B. Keppler, University of Vienna (Austria) and cultured in DMEM medium (PAA, Pasching, Austria) supplemented with 50 mg/ml gentamycin (Serva, Heidelberg, Germany) and 10% heat-inactivated fetal bovine serum (PAA). The cells were cultured in a humidified atmosphere (37°C, 5% CO₂). The cells were seeded in 96-well plates at a density of 1.5 × 10³ cells/well, incubated overnight, and treated with a range of concentrations (0–50 μM) of metallohelices for 72 h. At the end of the treatment, 10 μl MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; 2.5 mg/ml)] was added, and the cells were cultured for another 4 hours. The medium was then removed, and the formazan products were dissolved in DMSO. Absorbance at 570 nm (versus 620 nm) was measured with Absorbance Reader (Spark, TECAN, Switzerland). IC₅₀ values refer to the compound concentration inducing 50% cell growth inhibition.

Malina J., Kostrhunova H., Scott P, & Brabec V. (2023). Metallohelices stabilize DNA three-way junctions and induce DNA damage in cancer cells. Nucleic Acids Research, 51(14), 7174-7183.

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Culturing and Transfecting C2C12 and ARPE-19 Cells

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Cells were maintained in a humidified atmosphere containing 5% CO2 at 37°C and subcultured every two to three days. Culture medium consisted of DMEM/F12 supplemented with GlutaMAX, 4.5 mg/ml D-glucose (high glucose) (Thermo Fisher/Gibco #31331-093), 10% fetal bovine serum (FBS, Biowest) and 50 mg/ml gentamycin (Serva). For transfection, cells were seeded on 12 mm glass coverslips in a 24-well plate at a density of 10.000 (C2C12) or 50.000 cells (ARPE-19)/well. For centriole analysis, cells were fixed 24 h after transfection.

Steinfeldt J., Becker R., Vergarajauregui S., & Engel F.B. (2021). Alternative splicing of pericentrin contributes to cell cycle control in cardiomyocytes.

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