Tib 49

The murine AML cell line TIB-49 was purchased from the American Type Culture Collection. For all experiments, cell lines were transduced with luciferase/Mcherry using a lentiviral vector (pCDH-EF-eFFLy-T2A-mCherry). Murine LSK cells were obtained from transgenic C57BL/6J mice expressing mIDH2 (IDH2R140Q) and subsequently transduced with Hoxa9-GFP and Meis1a-YFP oncogenes, as previously described.^{18 (link)} Further details are provided in the Online Supplementary Methods.

Wild-type C1498 mouse leukemia cells (TIB-49, American Type Culture Collection) were cultured at 37°C in 5% CO₂ with Dulbecco’s modified Eagle’s medium [DMEM; with l-glutamine, glucose (4.5 g/liter), and sodium pyruvate; Corning] supplemented with 10% bovine growth serum (BGS; Hyclone) and 1% penicillin-streptomycin (Pen-Strep; Gibco). Engineered C1498-VCAM cells were cultured with DMEM supplemented with 10% U.S. Department of Agriculture (USDA) fetal bovine serum (FBS; Omega Scientific), 1% Pen-Strep, and hygromycin B (400 μg/ml; InvivoGen). Engineered C1498-VLA cells were cultured with DMEM supplemented with 10% USDA FBS, 1% Pen-Strep, and puromycin (1 μg/ml; InvivoGen). bEnd.3 mouse brain endothelial cells (CRL-2299, American Type Culture Collection) were cultured with DMEM supplemented with 10% BGS and 1% Pen-Strep. AmphoPhoenix cells (obtained from the National Gene Vector Biorepository) were cultured with DMEM supplemented with 10% BGS and 1% Pen-Strep. DC2.4 mouse dendritic cells (SCC142, Sigma-Aldrich) were cultured with DMEM supplemented with 10% BGS and 1% Pen-Strep.