Cells were digested with trypsin, washed twice with phosphate-buffered saline (PBS) and collected into a centrifuge tube. Binding buffer was added to prepare cell suspensions with a final concentration of 1 × 10 6 /mL. Annexin V-FITC kit (BioVision, Milpitas, USA) instructions were used for labeling, which was performed according to the instructions of Annexin V-FITC kit. Annexin V was added for staining at room temperature in the dark for 15 min.
Annexin 5 fitc kit
Manufactured by Abcam
Sourced in United States, United Kingdom
The Annexin V-FITC kit is a product designed for the detection and analysis of apoptosis. Annexin V, a protein that binds to phosphatidylserine, is conjugated to the fluorescent dye FITC, allowing for the visualization of apoptotic cells. The kit provides the necessary reagents and protocols for performing these assays.
Automatically generated - may contain errors
Lab products found in correlation
Rnase a, by Merck Group (4 mentions)
Propidium iodide, by Merck Group (3 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (3 mentions)
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Facscalibur, by BD (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Enhanced chemiluminescence western blotting detection reagent, by Cytiva (2 mentions)
Cm h2dcfda, by Thermo Fisher Scientific (2 mentions)
Facscan cytometer, by BD (2 mentions)
Gant61, by Merck Group (2 mentions)
Ab49314, by Abcam (2 mentions)
Coulter epics xl, by Beckman Coulter (1 mentions)
Cellquest pro software, by BD (1 mentions)
Modfit lt, by Verity Software House (1 mentions)
Anti htert antibody, by Abcam (1 mentions)
Bibr 1532, by Cayman Chemical (1 mentions)
Enhanced chemiluminescence western blotting detection reagent, by GE Healthcare (1 mentions)
Fortessa x20, by BD (1 mentions)
Dfc425, by Leica (1 mentions)
Taxol, by Merck Group (1 mentions)
45 protocols using annexin 5 fitc kit
1
Annexin V-FITC Cell Apoptosis Assay
2
Analysis of CTN06-induced Cell Cycle Arrest and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PC3 cells were treated with 0.1% DMSO (control) and CTN06 at the indicated concentrations for 24 h. Cell-cycle arrest was determined by the incorporation of propidium iodide (Sigma-Aldrich) into permeabilized cells. Cells undergoing apoptosis were identified using an Annexin V-FITC kit (Abcam), following the manufacturer's instructions. The cells were analyzed using a Coulter Epics XL flow cytometer (Beckman Coulter, Miami, FL, USA).
3
Cell Cycle and Apoptosis Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cycle analysis was performed by flow cytometry. Cells were harvested with trypsinization together with floating non-viable cells. The cells were washed once with PBS and suspended in sodium citrate buffer (40 mM Na-Citrate, 0.3% Triton X-100, 0.05 mg/ml propidium iodide, PBS) 20 minutes prior to analysis. Cell cycle analysis was performed using FACSCalibur (Becton Dickinson, CA, USA) and CellQuest Pro software (Becton Dickinson). Cell cycle and apoptosis analyses were performed with ModFit LT (Verity Software House, Inc., Topsham, ME, USA) and Flowing Software ver. 2.5 (Mr. Perttu Terho, Turku Centre for Biotechnology, Finland, www.flowingsoftware.com ), respectively. To further analyze the apoptosis induction after MX and MX + imatinib treatment HeLa cells were grown on 6-well plates and treated with indicated drugs for 24 and 48 hours. Medium and cells were collected and the samples were stained with Annexin-V-FITC kit (ab14085; Abcam, Cambridge, UK) according to manufacturer's instructions. Data were acquired with a FACSCalibur flow cytometer, and analyzed with Flowing Software.
4
Annexin V Flow Cytometry Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Annexin V assays were performed using an Annexin V/FITC kit from Abcam (ab14085) following the manufacturer's instructions. Briefly, cells were collected and counted. Then, 1 × 105 cells were mixed with 500 μl Annexin V binding buffer, 5 μl Annexin V-FITC and 5 μl propidium iodide. The mixture was incubated for 5 mins at room temperature in the dark. The Annexin V-FITC signal was then analysed via flow cytometry (Ex=488 nm, Em=530 nm) using a FITC signal detector and a phycoerythrin emission signal detector to detect PI staining.
5
Endometrial Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The endometrial cancer cell lines, ECC-1 and Ishikawa, were used. The ECC-1 cell line was maintained in RPMI 1640 supplemented with 5% FBS, 300mM L-glutamine, 5 μg/ml bovine insulin, 10,000 U/ml penicillin and 10,000 μg/ml streptomycin. Ishikawa cells were grown in MEM supplemented 5% FBS, 300 mM L-glutamine, 10,000 U/ml penicillin and 10,000 μg /ml streptomycin. Both the cell lines were cultured at 37°C in a humidified atmosphere with 5% CO2. MTT (3-(,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and RNase A were purchased from Sigma (St. Louis, MO). The anti-MMPs and anti-α-tubulin antibodies were purchased from Cell Signaling (Beverly, MA). The anti-hTERT antibody and Annexin V FITC kit were purchased from Abcam (Cambridge, MA). The Enhanced chemiluminescence Western blotting detection reagents were purchased from GE Health care (GE Healthcare Life Sciences, Piscataway, NJ). BIBR1532 was purchased from Cayman (Ann Arbor, MI) and dissolved in DMSO. All other chemicals were purchased from Sigma (St Louis, MO).
6
Apoptosis Assay in AML-12 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 12 h of OGD, FFA treated AML-12 cells were washed with cold PBS twice and were trypsinized. The cells were resuspended in 500 µL of 1× binding buffer (Annexin V- FITC kit Cat# ab14085, Abcam, Cambridge, MA, USA), followed by addition of five µL of Annexin V-FITC and five µL of Propidium iodide (PI) and incubated for 15 min in the dark at room temperature. The samples were analysed using flow cytometry (Fortessa X-20, BD Biosciences, San Jose, CA, USA) and results were analysed using FlowJo (Version 10.6.1, Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
7
Apoptosis Assay of Chemotherapeutic Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RT-4 and ARPE-19 cells were grown on round coverslips placed in 12-well plates and allowed to adhere for 16 h. The cells were incubated for 48 h either untreated or with chemotherapeutic agents with or without proTAME. The cell culture media was carefully removed, and the cells were washed three times with cold PBS. Annexin V-FITC Kit (Abcam, cat no: ab14085) was used to stain the cells according to the manufacturer’s protocol. A camera (DFC425, Leica) attached fluorescence microscope (Leica LM 4000B) was then used to examine the cells.
8
Apoptosis Detection in A549/MDR Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometry was performed on the A549/MDR cells using the Annexin V-FITC Kit (ab14085, Abcam, Cambridge, UK). In brief, cells were seeded in six-well plates at a density of 2 × 105 cells/well. After cell transfection, harvested cells were washed twice with PBS and suspended in binding buffer. Then cells were stained with the Annexin V-FITC/PI apoptosis detection kit and the apoptosis rates were measured using a flow cytometer (BD Biosciences, Bedford, MD, USA).
9
Cytotoxic Activity of Taxol on A2780cp Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A2780cp cell line (Human epithelial ovarian carcinoma) was purchased from NCBI (National Cell Bank of Iran). Trypsin/EDTA (1X) and fetal bovine serum were provided from Gibco (USA), RPMI-1640 and Trypan blue were purchased from Bio idea (Iran), and penicillin/streptomycin and phosphate buffer saline were purchased from PAA (Austria). PI (Propodium Iodide), DAPI (4′, 6-diamidino-2-phenylindole dihydrochloride) kit and MTT [3-(4, 5-dimethylthiozol-2-il) 2, 5 di phenyl tetrazolium bromide] and acridine orange/propodium iodide were purchased from Sigma (USA). Annexin V-FITC kit and Caspase-9 assay and Caspase-3 assay kit were prepared from Abcam (UK). Taxol or paclitaxel was purchased from Sigma (USA).
10
Annexin-V/FITC Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Annexin-V/FITC kit (Abcam Inc., Cambridge Science Park, Cambridge, UK) for apoptosis detection combined with 2-fluorescent-channel flow cytometry was used to determine apoptotic and necrotic cell populations as previously described [35 (link),37 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!