1st strand cdna synthesis kit
The 1st Strand cDNA Synthesis Kit is a laboratory tool used for the reverse transcription of RNA to complementary DNA (cDNA). This kit provides the necessary reagents and enzymes to convert RNA into single-stranded cDNA, which can then be used for various downstream applications such as gene expression analysis, cloning, and sequencing.
Lab products found in correlation
9 protocols using 1st strand cdna synthesis kit
Evaluating mRNA Expression Profiles in ccRCC
Reverse Transcription qPCR Protocol
RT-qPCR Analysis of Fungal Transcripts
Validation of RNA-Seq Transcriptome Profiling
Quantitative Analysis of miRNA and mRNA Expression in DVT Patients
Quantification of HOTAIR and miR-130a-3p
RNA Extraction and qPCR Analysis
Transcriptomic Analysis of M. oryzae
For qRT–PCR assays, we used the 1st Strand cDNA Synthesis Kit (Vazyme, China) to generate complementary DNA (cDNA). TransStart Tip Green qPCR SuperMix (TransGen Biotech, China) was used for qRT–PCR assays on the CFX Connect Real-time PCR System (Bio-Rad, USA). The β-tubulin gene (MGG_00604) was used as the internal control to calculate gene expression by the 2−ΔΔCt method. Each treatment was repeated at least three times with independent samples. The primers used are listed in
Cloning and Phylogenetic Analysis of NtSOS2 from Nitraria tangutorum
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