Cytotox 96 ldh release assay kit

Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads, anti-Myc (M5546), and anti-LPS (O11:B4) were purchased from Sigma-Aldrich. Anti-GSDMD (sc-393581) and anti-GAPDH (sc-47724) were from Santa Cruz Biotechnology. Anti-Ub (3936), anti-HA (3724), anti-β-actin (3700), and the secondary antibodies used for immunoblotting and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901) was purchased from Abcam, whereas Nigericin, Pam3CSK4 (tlrl-pms) were purchased from InvivoGen. ELISA kits for analysis of human IL-1β were purchased from MultiSciences. CytoTox 96 LDH-release assay kit (G1780) was purchased from Promega.

Anti-HA (3724), anti-GSDMD (39754) and anti-Acetylated Lysine (9441) antibodies were purchased from Cell Signaling Technology. Anti-GAPDH antibody, HRP-labeled goat anti-mouse IgG and goat anti-rabbit IgG were from Hangzhou Fudebio. Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads (10004D), anti-Myc (C3956) antibodies and LPS (O111:B4, L2630) were purchased from Sigma-Aldrich. Anti-FLAG antibody (rabbit source), Goat pAb to MS IgG (Chromeo 555, ab60316), Dnk pAb to Rb IgG (Alexa Fluor 647, ab150075) fluorescent secondary antibodies were obtained from Abcam. Nigericin, Pam3CSK4 (TLRL-PMS), poly(dA:dT) (TLRL-PATN) and flagellin (TLRL-EPSTFLA) were purchased from InvivoGen. ELISA kits for analysis of human IL-1β, mouse IL-1β, mouse IL-6 and mouse TNF-α were purchased from MultiSciences. CytoTox 96 LDH-release assay kit (G1780) was from Promega. Efficient eukaryotic transfection reagent VigoFect was purchased from Vigorous Biotechnology (Beijing), Lipofectamine 2000 transfection reagent was obtained from Invitrogen while Lipo8000 transfection reagent was obtained from Beyotime Biotechnology. Trichostain A (HY-15144), Nicotinamide (HY-B0150), LMK-235(HY-18998) and okadaic acid (HY-N6785) were purchased from MCE.