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α catenin

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α-catenin is a protein that plays a key role in cell-cell adhesion and the regulation of the actin cytoskeleton. It is a component of the cadherin-catenin complex, which is involved in the formation and maintenance of adherens junctions between cells.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Lsm 510 meta, by Zeiss (2 mentions) Irdye 680lt goat anti mouse igg, by LI COR (1 mentions) Ve cadherin, by Santa Cruz Biotechnology (1 mentions) Phospho mlc, by Cell Signaling Technology (1 mentions) Blebbistatin, by Merck Group (1 mentions) Low endotoxin bsa, by Merck Group (1 mentions) Pa p9767, by Merck Group (1 mentions) Irdye 800cw goat anti rabbit immunoglobulin g igg, by LI COR (1 mentions) Phospho yap s127, by Abcam (1 mentions) Alexa fluor 647 goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (1 mentions) Y 27632, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) α actinin, by Merck Group (1 mentions) β catenin, by BD (1 mentions) Gm130, by BD (1 mentions) Vimentin, by Santa Cruz Biotechnology (1 mentions) N cadherin, by Santa Cruz Biotechnology (1 mentions) E cadherin, by Thermo Fisher Scientific (1 mentions) Vimentin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti–α‐catenin Rabbit anti-α-catenin

4 protocols using α catenin

1

Immunoblotting and Immunofluorescence Assay Protocol

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Blebbistatin (B05060), low-endotoxin BSA (A8806), PA (P9767), and Y27632 (Y0503) were from Sigma Aldrich (St. Louis, MO), while cis-PO/palmitoleic acid (10009871) was from Cayman Chemicals (Ann Arbor, MI). Antibodies used for immunoblotting were α-actinin (Millipore Sigma; A7811), β-actin (Cell Signaling; 4790), α-catenin (ThermoFisher; 13-9700), β-catenin (BD Biosciences; 610153), VE-cadherin (Santa Cruz; 9989), RhoA (Santa Cruz; c-418), IRDye 800CW goat anti-rabbit immunoglobulin G (IgG) (LI-COR; 9253211), and IRDye 680LT goat anti-mouse IgG (92668070). Antibodies and reagents used for immunofluorescence were α-catenin (ThermoFisher; 13-9700), β-catenin (BD Biosciences; 610153), DAPI (4’,6-Diamidino-2-phenylindole, dihydrochloride; ThermoFisher; D1306), GM130 (BD Biosciences; 610823), phospho-MLC (Cell Signaling; 3674), phalloidin (Alexa Fluor 555) (ThermoFisher; A34055), phospho-YAP S127 (Abcam; 76252), YAP (Cell Signaling 14074), VE-cadherin (Santa Cruz; 9989), goat anti-rabbit IgG secondary antibody (Alexa Fluor 488) (ThermoFisher; A11008), goat anti-mouse IgG secondary antibody (Alexa Fluor 647) (ThermoFisher; A21235). Dako fluorescence mounting medium (S3023) was from Aligent. Y227632 (Y0503) was from Millipore Sigma (Burlington, MA). The plasmid encoding GFP–β-actin was kindly provided by Sergio Grinstein (Hospital for Sick Children, Toronto, Ontario, Canada).
Tokarz V.L., Pereira R.V., Jaldin-Fincati J.R., Mylvaganam S, & Klip A. (2023). Junctional integrity and directional mobility of lymphatic endothelial cell monolayers are disrupted by saturated fatty acids. Molecular Biology of the Cell, 34(4), ar28.
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2

Immunofluorescence Analysis of Epithelial-Mesenchymal Transition Markers

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A549 cells were cultured on cover glasses, fixed using 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100 in TBS. The cover glasses were incubated with the primary antibodies (E-cadherin, α-catenin, vimentin and N-cadherin, Santa Cruz Biotechnology) at 1:50 dilutions. E-cadherin, α-catenin, vimentin and N-cadherin were detected with an anti-goat secondary antibody conjugated to Alexa Fluor 488 (Invitrogen Life Technologies). The fluorescent staining was visualized using a 63× NA 1.3 oil objective on a confocal microscope (LSM 510 Meta; Carl Zeiss, Inc.). The images were captured with sequential acquisition settings at a resolution of 512 × 512 pixels with a 12-bit depth. The confocal settings were fixed for the duration of the experiments to enable the comparisons of the fluorescence intensities.
Ye Z., Yin S., Su Z., Bai M., Zhang H., Hei Z, & Cai S. (2016). Downregulation of miR-101 contributes to epithelial-mesenchymal transition in cisplatin resistance of NSCLC cells by targeting ROCK2. Oncotarget, 7(25), 37524-37535.
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3

Co-culture Assay for Cell-Cell Interactions

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The hCMVECs were plated in collagen-coated 96-well plates (cat# 161093, Invitrogen, Carlsbad, CA, USA) and allowed to grow for 48 h, after which CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate, cat# C7025, Invitrogen)-stained NZM cells (1 µM) were added at a 1:10 ratio. Cells were incubated in co-culture for 30 min. Fixation and permeabilisation were performed with 4% paraformaldehyde and 0.1% Triton X in PBS, respectively. Blocking was performed using 1% bovine serum albumin (BSA). Cells were stained for α-catenin (dilution 1:100, cat# 13-9700, Invitrogen).
Anchan A., Kalogirou-Baldwin P., Johnson R., Kho D.T., Joseph W., Hucklesby J., Finlay G.J., O’Carroll S.J., Angel C.E, & Graham E.S. (2019). Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology. Biosensors, 9(2), 56.
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4

Immunofluorescence Labeling of Neural Organoids

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Neural organoids were fixed with 4% paraformaldehyde (PFA) at room temperature for 40 min, dehydrated and embedded in paraffin blocks. Sections 10 μm-thick were obtained, rehydrated, blocked with buffer containing 1% bovine serum albumin for 45 min at 23 °C and incubated with primary antibodies: FOLR1, 1:250 (Bioworld Technologies, cat. # BS386); SOX2, 1:200 (R&D Systems, cat. # AF2018); pan-cadherin, 1:300 (Abcam, cat. # ab22744); α-catenin, 1:250 (Invitrogen, cat. # 13-9700) in blocking buffer overnight at 4 °C, followed by incubation with fluorescently tagged Alexa Fluor secondary antibodies: anti-mouse 647 donkey, 1:300 (Invitrogen, cat. # A31571), anti-rabbit 488 donkey (Invitrogen, cat. # A21206) and anti-goat 596 donkey (Invitrogen, cat. # A11057) for 1 h at 23 °C. Imaging of 20–25 sections per fluorescently labeled neural organoid was done under a confocal microscope (Nikon A1) through a 1 μm-step z-stack scanning.
Balashova O.A., Panoutsopoulos A.A., Visina O., Selhub J., Knoepfler P.S, & Borodinsky L.N. (2024). Noncanonical function of folate through folate receptor 1 during neural tube formation. Nature Communications, 15, 1642.
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