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Stellaris fish probe set

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The Stellaris FISH Probe set is a collection of fluorescently labeled DNA probes designed for use in Fluorescence in situ Hybridization (FISH) applications. These probes are used to detect and visualize specific RNA or DNA targets within cells or tissue samples.

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Lab products found in correlation

Stellaris fish probe designer, by Biosearch Technologies (3 mentions) Quasar 570, by Biosearch Technologies (1 mentions)

4 protocols using stellaris fish probe set

1

Transcriptomic Analysis of Drosophila Wing Disc

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Custom probes were designed against Ama‐RA coding sequence by utilizing the Stellaris. FISH Probe Designer v4.2 (Biosearch Technologies, Inc., Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner. The following parameters were used Oligo length = 18; min. spacing length = 2; masking level = 5. The designer generated 38 probes.
The wing discs were hybridized with the Ama Stellaris FISH Probe set labelled with Quasar 670 fluorophores dye (Biosearch Technologies, Inc.), following the manufacturer's instructions available online as “Protocol for D. Melanogaster Wing Imaginal Discs” at https://biosearchassets.blob.core.windows.net/assets/bti_custom_stellaris_drosophila_protocol.pdf.
Zappia M.P., de Castro L., Ariss M.M., Jefferson H., Islam A.B, & Frolov M.V. (2020). A cell atlas of adult muscle precursors uncovers early events in fibre‐type divergence in Drosophila. EMBO Reports, 21(10), e49555.
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2

Fos-B Expression in FUS H517D MNs

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Custom Stellaris® FISH Probes were designed against human Fos-B (NM_006732) using the Stellaris® FISH Probe Designer (LGC Biosearch Technologies, Inc., Petaluma, CA), which is available online (www.biosearchtech.com/stellarisdesigner). Control and FUSH517D/H517D hiPSC-derived MNs were hybridized with the Fos-B Stellaris FISH Probe set labeled with Quasar 570 (LGC Biosearch Technologies, Inc.), at 10 DPP according to the manufacturer's instructions (available online at www.biosearchtech.com/support/resources/stellaris-protocols).
Akiyama T., Suzuki N., Ishikawa M., Fujimori K., Sone T., Kawada J., Funayama R., Fujishima F., Mitsuzawa S., Ikeda K., Ono H., Shijo T., Osana S., Shirota M., Nakagawa T., Kitajima Y., Nishiyama A., Izumi R., Morimoto S., Okada Y., Kamei T., Nishida M., Nogami M., Kaneda S., Ikeuchi Y., Mitsuhashi H., Nakayama K., Fujii T., Warita H., Okano H, & Aoki M. (2019). Aberrant axon branching via Fos-B dysregulation in FUS-ALS motor neurons. EBioMedicine, 45, 362-378.
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3

Detecting ADRAM in Neurons and Brain

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Custom Stellaris® FISH Probes (Table S4) were designed against ADRAM using the Stellaris® FISH Probe Designer (Biosearch Technologies). The cultured primary neurons and adult mouse brain sections were hybridized with the ADRAM Stellaris FISH Probe set labeled with TAMRA (Biosearch Technologies, Inc.), following the manufacturer’s instructions.
Wei W., Zhao Q., Wang Z., Liau W.S., Basic D., Ren H., Marshall P.R., Zajaczkowski E.L., Leighton L.J., Madugalle S.U., Musgrove M., Periyakaruppiah A., Shi J., Zhang J., Mattick J.S., Mercer T.R., Spitale R.C., Li X, & Bredy T.W. (2022). ADRAM is an experience-dependent long noncoding RNA that drives fear extinction through a direct interaction with the chaperone protein 14-3-3. Cell reports, 38(12), 110546.
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4

Detecting ADRAM in Neurons and Brain

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Custom Stellaris® FISH Probes (Table S4) were designed against ADRAM using the Stellaris® FISH Probe Designer (Biosearch Technologies). The cultured primary neurons and adult mouse brain sections were hybridized with the ADRAM Stellaris FISH Probe set labeled with TAMRA (Biosearch Technologies, Inc.), following the manufacturer’s instructions.
Wei W., Zhao Q., Wang Z., Liau W.S., Basic D., Ren H., Marshall P.R., Zajaczkowski E.L., Leighton L.J., Madugalle S.U., Musgrove M., Periyakaruppiah A., Shi J., Zhang J., Mattick J.S., Mercer T.R., Spitale R.C., Li X, & Bredy T.W. (2022). ADRAM is an experience-dependent long noncoding RNA that drives fear extinction through a direct interaction with the chaperone protein 14-3-3. Cell reports, 38(12), 110546.
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