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2 protocols using isopropyl alcohol

1

Peptide Synthesis Protocol Optimized

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The following reagents, 9-fluorenylmethoxycarbonyl (Fmoc) protected aa, O-(Benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate (TBTU), N,N′-dicyclohexylcarbodiimide (DDC) and 1-hydroxybenzotrazole (HOBt), were purchased from AAPPTEC (Louisville, KY, USA), ChemMatrix resin (0.60 mmol/g) from PCAS BiomMatrix Inc. (Saint-Jean-sur-Richelieu, QC, Canada), N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIS), Triton X-100, α-cyano-4-hydroxycinnamic acid (HCCA), methanol and absolute ethanol from Sigma-Aldrich (St. Louis, MO, USA), piperidine, pyridine, phenol, potassium cyanide, isopropyl alcohol (IPA), acetonitrile (ACN), N-methyl-2-pyrrolidone (NMP), trifluoroacetic acid (TFA) and diethyl ether from Panreac (Barcelona, Spain), acetic anhydride (Ac2O), ninhydrin and ethanedithiol (EDT) from Merck (Boston, MA, USA) and dichloromethane (DCM) from The Dow Chemical Company (Bogotá, Colombia).
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2

Nanoparticle Formulation for Targeted Delivery

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Dexamethasone, melatonin and coenzyme Q10 were supplied by Sigma-Aldrich (St.
Louis Mo., USA) at the highest available purity. Poly(D,L-lactide-co-glycolide) (PLGA) 50:50 (Mwt 35,000 g/mol) was purchased from Evonik España (Granollers, Spain).
Polyvinyl alcohol 67,000 g/mol (PVA) was supplied by Merck KGaA (Darmstadt, Germany). Isopropyl alcohol, methanol and acetonitrile, HPLC grade (PanReac AppliChem, Barcelona, Spain) were used to prepare the mobile phases in HPLC determinations. All other chemicals were reagent grade and used as received.
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