13c2 pfhxa

Manufactured by Wellington Laboratories

Sourced in Canada

13C2-PFHxA is a chemical standard used for analytical purposes. It serves as a reference compound for the identification and quantification of perfluorohexanoic acid (PFHxA) in various environmental and analytical samples.

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PFHxA (5 citations)

6 protocols using 13c2 pfhxa

Quantifying Perfluoroalkyl Substances in Solutions

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Calibration solutions were created by combining 2 solutions produced by the NIST reference materials 8446 Perfluorinated Carboxylic Acids and Perfluorooctane Sulfonamide in Methanol and 8447 Perfluorinated Sulfonic Acids in Methanol. Together, the solution contained 15 PFAAs as follows: PFBA, perfluoropentanoic acid (PFPeA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), PFOA, perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUnA), perfluorododecanoic acid (PFDoA), perfluorotridecanoic acid (PFTriA), perfluorotetradecanoic acid (PFTA), PFBS, perfluorohexanesulfonic acid (PFHxS), PFOS, and perfluorooctanesulfonamide (PFOSA).
Internal standards were purchased from Cambridge Isotope Laboratories, RTI International, and Wellington Laboratories to create an internal standard mixture comprised of 11 isotopically labeled PFAAs, as follows: ¹³C₄-PFBA, ¹³C₂-PFHxA, ¹³C₈-PFOA, ¹³C₉-PFNA, ¹³C₉-PFDA, ¹³C₂-PFUnA, ¹³C₂-PFDoA, ¹⁸O₂-PFBS, ¹⁸O₂-PFHxS, ¹³C₄-PFOS, and ¹⁸O₂-PFOSA.

Bangma J.T., Bowden J.A., Brunell A.M., Christie I., Finnell B., Guillette M.P., Jones M., Lowers R.H., Rainwater T.R., Reiner J.L., Wilkinson P.M, & Guillette LJ J.r. (2016). PERFLUORINATED ALKYL ACIDS IN PLASMA OF AMERICAN ALLIGATORS (ALLIGATOR MISSISSIPPIENSIS) FROM FLORIDA AND SOUTH CAROLINA. Environmental toxicology and chemistry, 36(4), 917-925.

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Quantitative Analysis of Perfluoroalkyl Substances

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Twelve PFASs (standards purchased from
Sigma-Aldrich) were analyzed, i.e., C₃–C₅ and C₇–C₉ perfluoroalkyl carboxylates
(PFCAs), C₄, C₆, and C₈ perfluoroalkyl
sulfonates (PFSAs), perfluorooctane sulfonamide (FOSA), and 6:2 and
8:2 fluorotelomer sulfonates (6:2 and 8:2 FTSA). For quantification
as well as for quality assurance and control (QA/QC), eight mass-labeled
internal standards (ISs) (i.e., ¹³C₄-PFBA, ¹³C₂-PFHxA, ¹³C₄-PFOA, ¹³C₅-PFNA, ¹³C₂-PFDA, ¹⁸O₂-PFHxS, ¹³C₄-PFOS, ¹³C₈-FOSA, purities > 99%, Wellington Laboratories,
Guelph, ON) were also included. For details on the included PFASs
and their internal standards, see Table S1 in the Supporting Information.

Campos-Pereira H., Kleja D.B., Sjöstedt C., Ahrens L., Klysubun W, & Gustafsson J.P. (2020). The Adsorption of Per- and Polyfluoroalkyl Substances (PFASs) onto Ferrihydrite Is Governed by Surface Charge. Environmental Science & Technology, 54(24), 15722-15730.

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Perfluorinated Compound Analysis Protocol

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In this study, 11 PFCs were selected for target compounds, including 7 PFCAs (4A to 8A) and 4 PFSAs (4S, 6S, 8S and F-53B) (detailed in Supplementary Information). ¹³C₄-PFBA, ¹³C₅-PFPeA, ¹³C₂-PFHxA, ¹³C₄-PFHpA, ¹³C₄-PFOA, ¹³C₅-PFNA, ¹³C₂-PFDA, ¹³C₄-PFBS, ¹³C₃-PFHxS(Na), ¹³C₄-PFOS(Na) and ¹³C₈-PFOS(Na) were purchased from Wellington Laboratories Inc. (ON, Canada) with purities of >98% as internal standards. F-53B was obtained from Shanghai Synica Co., Ltd. (Shanghai, China). Solvents for cleaning and extraction purposes were supplied by J.T. Baker (NJ, USA) and ultrapure water was used (18 MΩ·cm, Millipore, MA).

Zhang Y.Z., Wang B., Wang W., Li W.C., Huang J., Deng S.B., Wang Y.J, & Yu G. (2016). Occurrence and source apportionment of Per- and poly-fluorinated compounds (PFCs) in North Canal Basin, Beijing. Scientific Reports, 6, 36683.

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Quantitative Analysis of Perfluorinated Compounds

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Calibration solutions were created by combining two solutions produced by the NIST Reference Materials (RMs) 8446 Perfluorinated Carboxylic Acids and Perfluorooctane Sulfonamide in Methanol and RM 8447 Perfluorinated Sulfonic Acids in Methanol. Together, the solution contained 15 PFAAs as follows: perfluorobutyric acid (PFBA), perfluoropentanoic acid (PFPeA), perfluorohexanoic acid (PFHxA), perfluoroheptanoic acid (PFHpA), PFOA, PFNA, perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUnA), perfluorododecanoic acid (PFDoA), perfluorotridecanoic acid (PFTriA), perfluorotet-radecanoic acid (PFTA), perfluorobutanesulfonic acid (PFBS), perfluorohexanesulfonic acid (PFHxS), PFOS, and perfluorooc-tanesulfonamide (PFOSA).
Internal standards (ISs) were purchased from Cambridge Isotope Laboratories (Andover, MA), RTI International (Research Triangle Park, NC), and Wellington Laboratories (Guelph, Ontario) to create an IS mixture comprised of eleven isotopically labeled PFAAs, and they were as follows: ¹³C₄-PFBA, ¹³C₂-PFHxA, ¹³C₈-PFOA, ¹³C₉-PFNA, ¹³C₉-PFDA, ¹³C₂-PFUnA, ¹³C₂-PFDoA, ¹⁸O₂-PFBS, ¹⁸O₂-PFHxS, ¹³C₄-PFOS, and ¹⁸O₂-PFOSA.

Tipton J.J., Guillette LJ J.r., Lovelace S., Parrott B.B., Rainwater T.R, & Reiner J.L. (2017). Analysis of PFAAs in American alligators part 1: Concentrations in alligators harvested for consumption during South Carolina public hunts. Journal of environmental sciences (China), 61, 24-30.

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Quantifying Perfluorinated Compounds in Samples

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The target PFASs comprised: six perfluoroalkyl carboxylates (PFCAs), namely perfluorobutanoate (PFBA), perfluorohexanoate (PFHxA), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), perfluorodecanoate (PFDA), and perfluoroundecanoate (PFUnDA); two perfluoroalkyl sulfonates (PFSAs), namely perfluorohexane sulfonates (PFHxS) and perfluorooctane sulfonates (PFOS); and one perfluorooctanesulfonamide (FOSA) (purity >99%, Wellington Laboratories, Guelph, ON). A total of nine isotopically labeled internal standards (IS) were used: ¹³C₄-PFBA, ¹³C₂-PFHxA, ¹³C₄-PFOA, ¹³C₅-PFNA, ¹³C₂-PFDA, ¹³C₂-PFUnDA, ¹⁸O₂-PFHxS, ¹³C₄-PFOS, and ¹³C₈-FOSA (purity >99%, Wellington Laboratories, Guelph, ON).

Sörengård M., Lindh A.S, & Ahrens L. (2020). Thermal desorption as a high removal remediation technique for soils contaminated with per- and polyfluoroalkyl substances (PFASs). PLoS ONE, 15(6), e0234476.

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Quantification of Perfluorinated Compounds

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Perfluorooctane sulfonamide (PFOSA); a mixture of PFBA, perfluoro-n-pentanoic acid (PFPeA), perfluoro-n-hexanoic acid (PFHxA), perfluoro-n-heptanoic acid (PFHpA), PFOA, perfluoro-n-nonanoic acid (PFNA), perfluoro-n-decanoic acid (PFDA), perfluoro-n-undecanoic acid (PFUnDA), perfluoro-n-dodecanoic acid (PFDoDA), perfluoro-n-tetradecanoic acid (PFTrDA), perfluoro-n-tetradecanoic acid (PFTeDA), perfluoro-n-hexadecanoic acid (PFHxDA), perfluoro-n-octadecanoic acid (PFOcDA), potassium perfluoro-1-butanesulfonate (PFBS), PFHxS, PFOS, and sodium perfluoro-1-decanesulfonate (PFDS); and mixture of ¹³C₄-PFOS, ¹³C₂-PFDA, ¹³C₅-PFNA, ¹³C₄-PFOA, ¹³C₄-PFBA, ¹³C₂PFHxA, ¹³C₂PFUnDA, ¹³C₂PFDoDA, and ¹⁸O₂PFHxS were purchased from Wellington Laboratories Inc. (Guelph, Ontario, Canada). Purities of the individual chemicals and mass-labeled chemicals are >98%. Oasis weak anion exchange solid-phase extraction cartridges (WAX; 6cc, 150 mg) were purchased from Waters Corp. (Milford, MA, USA). Methanol (LC-MS grade) was purchased from Merck Ltd. (Darmstadt, Germany), and ammonium solution (25%) was purchased from Sigma-Aldrich GmbH (Seelze, Germany). Milli-Q water was used during the experiment.

Lu G., Shao P., Zheng Y., Yang Y, & Gai N. (2022). Perfluoroalkyl Substances (PFASs) in Rivers and Drinking Waters from Qingdao, China. International Journal of Environmental Research and Public Health, 19(9), 5722.

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