Pl aqua gel oh 60

Manufactured by Agilent Technologies

Sourced in United States

The PL aqua gel-OH 60 is a high-performance gel filtration column for the separation and purification of biomolecules. It is designed for use in aqueous buffer systems and features a hydroxylated surface to provide excellent chemical stability and low non-specific binding.

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Lab products found in correlation

1200 series system, by Agilent Technologies (1 mentions) 1260 infinity, by Agilent Technologies (1 mentions) Pl aquagel oh 30, by Agilent Technologies (1 mentions)

2 protocols using pl aqua gel oh 60

Molecular Weight Characterization of Polysaccharide BLVP

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The
polysaccharide-extracted BLVP molecular weight was assessed by GPC
with an Agilent 1200 series system and a RID. Two columns connected
in series Agilent PL aqua gel-OH 60 and 30 (8 μm) were used
for the separation. Milli-Q water was used as the mobile phase at
1 mL/min. The RID was calibrated with Dextran standards solution (MW
(g/mol): 1,400,000, 150,000, 250,000, 10,000, and 1000). The polysaccharide
extract BLVP (3 mg ) was solubilized in 1 mL water, and then, 100
μL was injected for analysis.^{93 (link)}

Dhahri M., Sioud S., Dridi R., Hassine M., Boughattas N.A., Almulhim F., Al Talla Z., Jaremko M, & Emwas A.H. (2020). Extraction, Characterization, and Anticoagulant Activity of a Sulfated Polysaccharide from Bursatella leachii Viscera. ACS Omega, 5(24), 14786-14795.

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Molecular Weight Analysis of EPS

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We determined the molecular weight of EPS by high-performance gel permeation chromatography (HP-GPC; Agilent Technologies 1260 Infinity) equipped with columns PL-aquagel-OH 60 and PL-aquagel-OH 30 connected in series (length 300 mm and particle size 15 and 8 μm, respectively) (Agilent, United States). The HP-GPC was equipped with a differential refractometer and a UV detector, which was set at 280 nm. For the elution, 0.1 M of NaNO₃ and NaN₃ (0.02% w/v) (Honeywell Fluka^TM, United States) were used. The eluent was degassed and filtered through 0.1 μm pore size membrane filters (MF-Millipore^TM, United States). The EPS samples were dissolved in eluent at 1 mg/ml concentration and filtered through 0.2 μm filters. A 100 μl of EPS solution was injected into the HP-GPC, flow rate was adjusted to 0.5 ml/min, and the temperature of the columns was maintained at 30°C. The polyethylene glycol standards were used to calibrate the system (EasiVial PEG/PEO, pre-weighted calibration kit, 2 ml, Agilent, United States). The HP-GPC analysis was performed in duplicate.

Rehman Z.U., Vrouwenvelder J.S, & Saikaly P.E. (2021). Physicochemical Properties of Extracellular Polymeric Substances Produced by Three Bacterial Isolates From Biofouled Reverse Osmosis Membranes. Frontiers in Microbiology, 12, 668761.

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