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2 protocols using prosapogenin a

1

Comprehensive Chemical Analysis of Fenugreek

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Trigonella foenum-graecum seeds from Shanxi Province of China were used in this study. The chemical standards of UDP-glucose, UDP-rhamnose, UDP-xylose, diosgenin, yamogenin, cholesterol, β-sitosterol, ergosterol, brassicasterol, campesterol, kaempferol, quercetin, myricetin, protopanoxadiol, ursolic acid, trillin, prosapogenin A (α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside-3-O-diosgenin), zingiberoside A1 (α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside-3-O-yamogenin), and dioscin (α-L-rhamnopyranosyl-(1 → 4)-[α-L-rhamnopyranosyl-(1 → 2)]-β-D-glucopyranoside-3-O-diosgenin) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The ruscogenin and stigmasterol standards were ordered from Chengdu HerbPurify Biotechnology Co., Ltd. (Chengdu, China). High performance liquid chromatography (HPLC)-grade acetonitrile or methanol (Thermo Fisher Scientific, United States) was used for HPLC and liquid chromatograph-mass spectrometer (LC-MS) analysis.
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2

Extraction and Analysis of Ophiopogon Saponins

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All solvents and reagents used in extraction and analyses were of analytical grade. Formic acid, methanol, ethyl acetate, and acetic acid were purchased from Sigma. The reference compounds ophiopogonin A, ophiopogonin C, prosapogenin A, ophiopogonin D, and ophiopogonin D were obtained from Shanghai Yuanye Bio-Technology Co. Ltd. The purity of all reference compounds was > 98 %. The glass plates for HPTLC (silica gel 60 F254, 20 × 10 cm) were purchased from Merck Millipore.
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