Ad nfatc1
Ad-NFATc1 is a recombinant protein that functions as the active form of the transcription factor NFATc1. NFATc1 is a key regulator of gene expression involved in various cellular processes.
4 protocols using ad nfatc1
Adenoviral Vector Overexpression of NFATs
Adenoviral Vectors for Myoblast Regulation
Modulation of NFAT Transcription Factors in Myoblasts
The gene accession number of overexpressing-NFATc1/c2 is NM_172390 and NM_173091, respectively. Construction of NFATc3/c4 short hairpin RNA (shRNA) adenoviral vector were prepared as previously described 15 . These overexpression adenoviral vectors containing Ad-NFATc1, Ad-NFATc2, Ad-shNFATc3 and Ad-shNFATc4were obtained from Vigenebio.To con rm the role of NFATc3 or NFAtc4 on myoblast cells, the addition of Ad-shCtrl, Ad-shNFATc3, or Ad-shNFATc4 (1 × 10 9 pfu) into correspondingculture dish one day before the ISO was performed. And then these cells were replaced with differentiation medium for further observation.
Adenoviral Regulation of Myoblast
And then proliferation medium was replaced with differentiation medium for further observation.
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