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Trimethoprim sulphamethoxazole

Manufactured by HiMedia
Sourced in India

Trimethoprim/sulphamethoxazole is a combination antimicrobial agent that consists of two active ingredients: trimethoprim and sulphamethoxazole. It is commonly used in the treatment of various bacterial infections.

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3 protocols using trimethoprim sulphamethoxazole

1

Antimicrobial Susceptibility Testing

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Susceptibility testing of all donor isolates and the resulting transconjugants was performed by the disk diffusion method on Mueller Hinton agar as recommended by the Clinical and Laboratory Standard Institute [43 ]. Antibiotics tested were tetracycline (30 µg), gentamicin (30 µg), ciprofloxacin (5 µg) and trimethoprim/sulphamethoxazole (1.25/23.75 μg) (Hi-media, India).
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2

Antimicrobial Susceptibility Testing Protocol

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Antimicrobial susceptibility was determined by Kirby Bauer disc diffusion method on Mueller–Hinton Agar plates. Following antibiotics were used: amikacin (30 μg), gentamicin (10 μg), ciprofloxacin (30 μg), trimethoprim/sulphamethoxazole (1.25/23.75 μg), tigecycline (15 μg) (Hi Media, Mumbai). MIC’s of various antibiotics were also determined on Mueller–Hinton Agar plates by agar dilution method according to CLSI and EUCAST guidelines [16 , 17 ]. Following antibiotics were used: cefotaxime, ceftazidime, ceftriaxone, cefepime, imipenem, meropenem, ertapenem and aztreonam (Hi-Media, Mumbai, India).
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3

Antibiotic Susceptibility Testing Protocol

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Antimicrobial susceptibility was determined by Kirby–Bauer disc diffusion method13 (link) on MHA plates. The following antibiotics were used: amikacin (30 μg), gentamicin (10 μg), ciprofloxacin (30 μg), trimethoprim/sulphamethoxazole (1.25/23.75 μg), cefepime (30 μg), imipenem (10 μg), meropenem (10 μg), ceftriaxone (30 μg) and aztreonam (30 μg) (HiMedia). Minimum inhibitory concentrations (MICs) of various antibiotics were determined on MHA plates by agar dilution method according to CLSI guidelines14 using the following antibiotics: cefotaxime, ceftazidime, ceftriaxone, cefepime, imipenem, meropenem, ertapenem and aztreonam (HiMedia).
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