Chromogranin a

Manufactured by Nichirei Biosciences

Sourced in France, Germany

Chromogranin A is a glycoprotein used as a biomarker for neuroendocrine tumors. It is involved in the formation and secretion of catecholamine-containing secretory vesicles.

Automatically generated - may contain errors

Lab products found in correlation

P53 do 7, by Agilent Technologies (1 mentions) Kit cd117, by Agilent Technologies (1 mentions) Desmin d33, by Agilent Technologies (1 mentions)

2 protocols using chromogranin a

Histological Diagnosis of GI-SELs via FNA-CB and Resected Specimens

Check if the same lab product or an alternative is used in the 5 most similar protocols

All histocytological diagnoses for FNA-CB specimens and resected specimens were prospectively made via the consensus of two or more pathologists (YN, TS, MU, and FF). To assess the histology of the FNA-CB specimens, cell-block sections were prepared using a sodium alginate method and subjected to hematoxylin and eosin (HE) stain. Immunostaining was performed when the following situations occurred: 1) GI-SELs for which immunostaining would be diagnostic (e.g., GISTs, leiomyoma, schwannoma, etc.) were suspected based on HE staining, and 2) HE staining afforded an indeterminate histological diagnosis. The antibodies used for immunostaining were as follows: KIT (CD117; DAKO, Glostrup, Denmark), CD34 (NU-4A1; Nichirei, Tokyo, Japan), DOG1 (K9; Novocastra, Newcastle, UK), Desmin (D33; DAKO), αSMA (1A4; Enzo Life Sciences, Farmingdale, USA), Ki67 (MIB-1; Immunotech, Marseilles, France), P53 (DO-7; DAKO), MUC1 (Ma695; Novocastra), chromogranin A (chromogranin; Nichirei), and synaptophysin (27G12; Novocastra).
For resected specimens, HE staining and several immunostainings were performed using the method for FNA-CB specimens. When histological diagnoses for resected specimens could be made using HE staining, immunostaining was not performed.

Shimizu T., Koshita S., Ohira T., Harada Y., Kanno Y., Ogawa T., Yamagata T., Kusunose H., Sakai T., Tsuchiya T., Oikawa M., Noda Y., Sawai T, & Ito K. (2021). Endoscopic Ultrasonography-guided Fine-needle Aspiration Cytology Combined with a Cell-block Method for Gastrointestinal Subepithelial Lesions. Internal Medicine, 61(7), 935-942.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Tumor Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

After treatment, resected tumor tissues were formalin-fixed and paraffin-embedded, and paraffin blocks were sectioned at 4 μm for hematoxylin and eosin staining (HE staining), and for Ki-67, chromogranin A and synaptophysin immunohistochemical staining. Primary rabbit antibodies to Ki-67 (Leica Biosystems, Wetzlar, Germany), chromogranin A (Nichirei Biosciences, Tokyo, Japan) and synaptophysin (Epitomics, California USA) were used at dilutions of 1:5000, 1:1 and 1:10000, respectively. The Ki-67 labeling index was assessed by counting over 2,000 cells, and expressed as the percentage of positive cells in the most highly labeled regions.

Ohmoto A., Suzuki M., Takai E., Rokutan H., Fujiwara Y., Morizane C., Yanagihara K., Shibata T, & Yachida S. (2018). Establishment of preclinical chemotherapy models for gastroenteropancreatic neuroendocrine carcinoma. Oncotarget, 9(30), 21086-21099.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!