Cells lysates were collected in ice-cold RIPA Buffer (ThermoFisher) supplemented with Protease Inhibitor Cocktail (ThermoFisher). Total protein concentration was quantified with Bio-Rad DC protein assay reagent (Bio-Rad) using bovine serum albumin as the standard. Cell lysate or culture media was mixed in 5x XT sample buffer (Bio-Rad) and denatured at 95°C for 5 min to prepare the gel sample and loaded on 4%–12% Criterion XT Bis-Tris SDS-PAGE gels (Bio-Rad). The gel was transferred onto PVDF membrane (Millipore) on a Trans-Blot semi-dry transfer cell (Bio-Rad). Immunoblot was first blocked with Odyssey Blocking buffer (Li-Cor, Lincoln, ME), followed by incubation with rabbit anti-CERC1 (Novus Cat #NBP1-89238, Centennial, CO), rabbit anti-human PDGF-BB antibody (Abcam), Erk1/2 (Cell Signaling, Danvers, MA), or mouse anti-β-actin antibodies (Abcam), and detected with IRDye 800CW Goat anti-Rabbit (Li-Cor) or IRDye 680RD Donkey anti-Mouse (Li-Cor) secondary antibodies on the Odyssey scanner (LI-Cor).
Rabbit anti human pdgf bb antibody
Manufactured by Abcam
Rabbit anti-human PDGF-BB antibody is a primary antibody that specifically recognizes the PDGF-BB isoform of the platelet-derived growth factor protein in human samples. It is intended for use in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Erk1 2, by Cell Signaling Technology (2 mentions)
Mouse anti β actin antibody, by Abcam (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Odyssey blocking buffer, by LI COR (2 mentions)
Dc protein assay reagent, by Bio-Rad (2 mentions)
Xt sample buffer, by Bio-Rad (2 mentions)
Odyssey scanner, by LI COR (2 mentions)
Trans blot semi dry transfer cell, by Bio-Rad (2 mentions)
Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions)
Irdye 800cw goat anti rabbit, by LI COR (2 mentions)
Irdye 680rd donkey anti mouse, by LI COR (2 mentions)
Criterion xt bis tris sds page gels, by Bio-Rad (2 mentions)
2 protocols using rabbit anti human pdgf bb antibody
1
Protein Extraction and Western Blot Analysis
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells lysates were collected in ice-cold RIPA Buffer (ThermoFisher) supplemented with Protease Inhibitor Cocktail (ThermoFisher). Total protein concentration was quantified with Bio-Rad DC protein assay reagent (Bio-Rad) using bovine serum albumin as the standard. Cell lysate or culture media was mixed in 5x XT sample buffer (Bio-Rad) and denatured at 95°C for 5 min to prepare the gel sample and loaded on 4%–12% Criterion XT Bis-Tris SDS-PAGE gels (Bio-Rad). The gel was transferred onto PVDF membrane (Millipore) on a Trans-Blot semi-dry transfer cell (Bio-Rad). Immunoblot was first blocked with Odyssey Blocking buffer (Li-Cor, Lincoln, ME), followed by incubation with rabbit anti-CERC1 (Novus Cat #NBP1-89238, Centennial, CO), rabbit anti-human PDGF-BB antibody (Abcam), Erk1/2 (Cell Signaling, Danvers, MA), or mouse anti-β-actin antibodies (Abcam), and detected with IRDye 800CW Goat anti-Rabbit (Li-Cor) or IRDye 680RD Donkey anti-Mouse (Li-Cor) secondary antibodies on the Odyssey scanner (LI-Cor).
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