Compound light microscope

Manufactured by Zeiss

Sourced in Germany

The Compound Light Microscope is a scientific instrument designed to magnify and observe small objects or specimens. It utilizes a series of lenses to produce an enlarged image of the subject, allowing for detailed examination and analysis. The core function of this microscope is to provide a clear and high-resolution view of microscopic samples.

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Lab products found in correlation

Vibratome, by Leica (1 mentions) Paraformaldehyde (pfa), by Avantor (1 mentions) Permount, by Thermo Fisher Scientific (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Compound microscope (36 citations) Axioskop 2 compound light microscope (2 citations) ZEISS AXIO A1 Compound Light Microscope (2 citations) Axioskop compound light microscope (2 citations)

7 protocols using compound light microscope

Immunofluorescence Staining of Cultured Cells

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Immunofluorescence staining was performed on cells grown on 8-chambered slides (Fisher, catalog# 154534PK). The cells were fixed with 100% ice-cold methanol for 5 min at 4 °C. The slides were washed with 1X PBS 3 times for 5 min. The cells were then blocked in 1X PBS + 10% goat serum+0.1% BSA + 0.1 M glycine+0.1% Tween 20 (antibody block) for at least 30 min at room temperature. Primary antibodies were diluted with antibody block at a 1:100 dilution and added to the slides for 2 h. Slides were washed in 1X PBS three times for 5 min each, treated with Alexa Fluor secondary antibodies (diluted using the antibody block to a 1:500 dilution) for 1 h, and then washed three times for 5 min each. The slides were counterstained in DAPI (Fisher, catalog# ICN15757410) diluted at 1:10000 in distilled water for 10 min before imaging under a Zeiss Compound Light microscope. For each experiment, the control and treatment cells or the control and the ∆17 cells were seeded simultaneously on different chambers of the same slide. Processing, immunostaining, and imaging of slides were also performed simultaneously.

Lakhia R., Ramalingam H., Chang C.M., Cobo-Stark P., Biggers L., Flaten A., Alvarez J., Valencia T., Wallace D.P., Lee E.C, & Patel V. (2022). PKD1 and PKD2 mRNA cis-inhibition drives polycystic kidney disease progression. Nature Communications, 13, 4765.

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Avian Hematology Profiling Protocol

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Body weight and egg production were recorded on a weekly basis. Blood samples (5 mL) were obtained from the brachial vein prior to the experiment (week 47) as well as weeks 50, 53, 55, 57, 59, and 61 of age following weighing the birds. A fraction of each sample was collected in anticoagulant-free tubes to obtain serum samples and the remaining blood was collected in EDTA-coated tube to separate the plasmas. Prior to centrifugation, the whole blood samples were used to quantify the total erythrocyte and leukocyte number by hemocytometer, using the Natt–Herrick's solution (Buitenhuis et al., 2006 (link)). To determine the percentages of lymphocytes, monocytes, heterophils, eosinophils, basophils, and heterophil to lymphocyte (H:L) ratio, duplicate blood smears were air-dried, stained with Wright's-Giemsa (Saikin Kagaku Institute Co. Ltd., Sendai, Japan), and counted to a total of 100 cells/slide, using a Zeiss (Jena, Germany) compound light microscope (×1000 magnification).

Keshavarz R., Akhlaghi A., Zamiri M.J., Jafarzadeh Shirazi M.R., Saemi F., Akhlaghi A.A., Zhandi M., Afrouziyeh M, & Zuidhof M.J. (2019). The long-term oral administration of thyroxine: effects on blood hematological and biochemical features in broiler breeder hens. Poultry Science, 98(12), 7003-7008.

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Immunofluorescence Staining of Kidney Sections

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Following PFA fixation, the kidney tissues were washed three times with 1X PBS and then treated with 30% sucrose overnight. The samples were embedded in OCT and stored at −80 °C degrees until further use. The OCT blocks were cryo-sectioned at 5 um thickness and kept at −20 °C degrees. Before immunofluorescence staining, the slides were left at room temperature for 30 minutes. The slides were then washed in 1X PBS for 5 minutes. The slides were antigen retrieved with sodium citrate for anti-m6A, anti-pCreb, anti-pHH3, and anti-Yap1 antibodies. For anti-Mettl3, anti-GFP, and Biotinylated-DBA antibodies, the slides were antigen retrieved with 0.25% TritonX 100 at room temperature for 40 minutes. Subsequently, the slides were treated with sodium borohydride to quench autofluorescence for 40 minutes and with 1X PBS+10% goat serum+0.1% BSA (antibody block) for 1-2 hours at room temperature. Sections were incubated with primary antibodies (1:500 dilution) overnight. The next day, slides were washed with PBS, incubated with appropriate Alexa Fluor secondary antibodies (1:500 dilution) for 1 hour, and mounted using Vecta Shield containing Dapi. We imaged the slides using a Zeiss Compound Light microscope.

Ramalingam H., Kashyap S., Cobo-Stark P., Flaten A., Chang C.M., Hajarnis S., Hein K.Z., Lika J., Warner G.M., Espindola-Netto J.M., Kumar A., Kanchwala M., Xing C., Chini E.N, & Patel V. (2021). A methionine-Mettl3-N6-methyladenosine axis promotes polycystic kidney disease. Cell metabolism, 33(6), 1234-1247.e7.

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Histological Confirmation of Thalamic Electrode Implantation

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Implanted animals were transcardially perfused with 0.1% heparinized phosphate-buffered saline (PBS) (APP Pharmaceuticals, Lake Zurich, IL, U.S.A.) followed by 4% paraformaldehyde (JT Baker, Center Valley, PA, U.S.A.) in PBS. Brains were then removed and post-fixed for 3 days in 4% paraformaldehyde (PFA) in PBS at 4°C. Brains were washed thrice in PBS, placed in 2% agarose gel (American Bioanalytical, Natick, MA, U.S.A.) and cut at 100 µm on a Vibratome (Leica Microsystems, Wetzlar, Germany). Slices were mounted on polarized slides (ThermoScientific, Waltham, MA, U.S.A.) and stained with cresyl violet to confirm electrode location using a manufacturer-recommended protocol for reagents (FD NeuroTechnologies, Columbia, MD, U.S.A.). Slides were then briefly dried and coverslipped with Permount (Fisher Chemicals, Pittsburgh, PA, U.S.A.). Images were taken at 40× magnification on a compound light microscope (Carl Zeiss, Oberkochen, Germany), imaged with a digital camera (Motic, Hong Kong), and digitally stitched together (Microsoft Image Composite Editor, Redmond, WA, U.S.A.). Electrode locations for the central lateral thalamus were confirmed using the following landmarks: location ventral to the hippocampus in the dorsal thalamus, and lateral to the stria medullaris/habenula complex (Fig. S1B,C).

Gummadavelli A., Motelow J.E., Smith N., Zhan Q., Schiff N.D, & Blumenfeld H. (2014). Thalamic stimulation to improve level of consciousness after seizures: Evaluation of electrophysiology and behavior. Epilepsia, 56(1), 114-124.

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Tissue Preparation for Microscopic Analysis

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The experiments were performed at Wuhan Servicebio Technology (Wuhan, China) following a standard laboratory procedure [99 (link)]. The main shoot apex or leaf samples were dissected and then fixed in a formalin–acetic acid–alcohol (FAA) solution overnight. Tissues were dehydrated and cleared using a series of ethanol and/or xylene, and finally transferred to refined paraffin wax. Serial sections were cut using a microtome, mounted on slides, deparaffinized, and stained with toluidine blue. Images were captured using a compound light microscope (Zeiss, Oberkochen, Germany).

Peng S., Li P., Li T., Tian Z, & Xu R. (2023). GhCNGC13 and 32 Act as Critical Links between Growth and Immunity in Cotton. International Journal of Molecular Sciences, 25(1), 1.

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Mitochondrial Membrane Potential Analysis

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MitoTracker Red CMXRos (Thermo Fisher, catalog# M7512) was used to analyze the mitochondrial membrane potential in live cells. The lyophilized MitoTracker® product was dissolved in dimethylsulfoxide (DMSO) to a final concentration of 1 mM and stored at −20 °C in small aliquots. Cells grown to 40–70% confluency were washed with sterile PBS and then treated with regular DMEM serum-free media containing 100 nM MitoTracker for 8 min. Immediately thereafter, the media was replaced with regular growth media and imaged under a Zeiss Compound Light microscope. The images were taken at the same exposure time for the samples of the same experiment. The intensity of fluorescence is directly proportional to membrane potential.

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Haemonchus Worm Cuticular Ridge Analysis

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In the three provinces of Laos, one to four adult female Haemonchus worms were randomly chosen from each vulvar process type from individually infected goats. Thus, 270 worms (90 worms from each province) were examined for cuticular ridge patterns (number of ridges) in cross sections. Study of the cuticular ridge patterns was according to the protocol originally described by Lichtenfels et al. (1986) and Lichtenfels et al. (1994) with some minor modifications reported by Vongnady et al. (2020a Vongnady et al. ( , 2020b)) (link). The selected regions for study of the synlophe in each worm were at three main regions: the esophageal-intestinal junction (EI), 4 mm from the anterior end (4 mm), and mid-body (MB) (Figure 2a,b). The number of cuticular ridges in each position was observed under a compound light microscope (Carl Zeiss, Germany; Olympus Corporation, Japan). The references for the numbers of cuticular ridge patterns at positions EI, 4 mm, and MB used in this study were obtained from previous publications for three main species: H. contortus, H. placei, and H. similis, as shown in Table 2.

Vongnady K., Rucksaken R, & Mangkit B. (2021). Cuticular ridge patterns applied for identifying adult female worms of Haemonchus species with various vulvar morphological types in infected native goats in Laos. Tropical biomedicine, 38(3).

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